A new method for generating high purity motoneurons from mouse embryonic stem cells

Biotechnology and Bioengineering

Volumn 111, Issue 10, 2014, Pages 2041-2055

  Mccreedy, Dylan A ^a   Brown, Chelsea R ^a   Butts, Jessica C ^a   Xu, Hao ^a   Huettner, James E ^b   Sakiyama Elbert, Shelly E ^a  

^a WASHINGTON UNIVERSITY IN ST LOUIS   (United States)

^b WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Electrophysiology; Neural differentiation; Puromycin selection; Transcription factor

Indexed keywords

ANTIBIOTICS; CELL CULTURE; ELECTROPHYSIOLOGY; LABORATORIES; MAMMALS; NEUROLOGY; STEM CELLS; TRANSCRIPTION FACTORS;

ACETYLCHOLINESTERASE ACTIVITY; AMYOTROPHIC LATERAL SCLEROSIS; FUNCTIONAL CHARACTERIZATION; MOUSE EMBRYONIC STEM CELLS; NEURAL DIFFERENTIATIONS; PLURIPOTENT STEM CELLS; POPULATION HETEROGENEITY; PUROMYCIN SELECTION;

NEURONS;

ACETYLCHOLINESTERASE; CRE RECOMBINASE; NEOMYCIN; PUROMYCIN; ACYLTRANSFERASE; ANTINEOPLASTIC ANTIMETABOLITE; HB9 PROTEIN, MOUSE; HOMEODOMAIN PROTEIN; PROTEIN SYNTHESIS INHIBITOR; PUROMYCIN N-ACETYLTRANSFERASE; TRANSCRIPTION FACTOR;

AMYOTROPHIC LATERAL SCLEROSIS; ANIMAL CELL; ARTICLE; CELL COUNTING; CELL HETEROGENEITY; CELL MATURATION; CELL POPULATION; CELL SURVIVAL; CELL VIABILITY ASSAY; CONTROLLED STUDY; ELECTROPHYSIOLOGY; ELECTROPORATION; EMBRYONIC STEM CELL; ENHANCER REGION; GENE CASSETTE; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IN VITRO STUDY; MOTONEURON; NERVE CELL DIFFERENTIATION; NONHUMAN; PLURIPOTENT STEM CELL; POLYMERASE CHAIN REACTION; ANIMAL; CELL CULTURE TECHNIQUE; CELL ENGINEERING; CYTOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOUSE; NERVOUS SYSTEM DEVELOPMENT; PROCEDURES; TRANSGENE;

ACETYLTRANSFERASES; ANIMALS; ANTIMETABOLITES, ANTINEOPLASTIC; CELL CULTURE TECHNIQUES; CELL ENGINEERING; EMBRYONIC STEM CELLS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HOMEODOMAIN PROTEINS; MICE; MOTOR NEURONS; NEUROGENESIS; PROTEIN SYNTHESIS INHIBITORS; PUROMYCIN; TRANSCRIPTION FACTORS; TRANSGENES;

EID: 84906783790     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25260     Document Type: Article

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