Gene-targeted embryonic stem cells: Real-time PCR assay for estimation of the number of neomycin selection cassettes

Biological Procedures Online

Volumn 13, Issue 1, 2011, Pages

  Mancini, Cecilia ^a   Messana, Erika ^a   Turco, Emilia ^a,c   Brussino, Alessandro ^a   Brusco, Alfredo ^a,b  

^a UNIVERSITY OF TURIN   (Italy)

^b AOU San Giovanni Battista   (Italy)

^c Molecular Biotechnology Center   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; NEOMYCIN;

ARTICLE; COPY NUMBER VARIATION; EMBRYONIC STEM CELL; GENE CASSETTE; GENE INSERTION; GENE TARGETING; MOLECULAR CLONING; MOUSE; NONHUMAN; POSITION EFFECT; REAL TIME POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING;

MURINAE;

EID: 84858018476     PISSN: None     EISSN: 14809222     Source Type: Journal    
DOI: 10.1186/1480-9222-13-10     Document Type: Article

References (10)

1. High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors. Thomas KR, Deng C, Capecchi MR, Mol Cell Biol 1992 12 2919 2923 1620105
2. Short DNA sequences inserted for gene targeting can accidentally interfere with off-target gene expression. Meier ID, Bernreuther C, Tilling T, Neidhardt J, Wong YW, Schulze C, Streichert T, Schachner M, FASEB J 2010 24 1714 1724 10.1096/fj.09-140749 20110269
3. Joyner A, Gene targeting a practical approach New York: Oxford University Press 2000
4. Know your neighbors: three phenotypes in null mutants of the myogenic bHLH gene MRF4. Olson EN, Arnold HH, Rigby PW, Wold BJ, Cell 1996 85 1 4 10.1016/S0092-8674(00)81073-9 8620528 (Pubitemid 26116800)
5. Insertion of a targeting construct in a Hoxd-10 allele can influence the control of Hoxd-9 expression. Rijli FM, Dolle P, Fraulob V, LeMeur M, Chambon P, Dev Dyn 1994 201 366 377 10.1002/aja.1002010408 7894075
6. Long-range disruption of gene expression by a selectable marker cassette. Pham CT, MacIvor DM, Hug BA, Heusel JW, Ley TJ, Proc Natl Acad Sci USA 1996 93 13090 13095 10.1073/pnas.93.23.13090 8917549 (Pubitemid 26382774)
7. Sambrook J, Russell D, Molecular Cloning: A Laboratory Manual Cold Spring Harbor Laboratory Press 2001
8. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Livak KJ, Schmittgen TD, Methods 2001 25 402 408 10.1006/meth.2001.1262 11846609 (Pubitemid 34164012)
9. High-throughput engineering of the mouse genome coupled with high-resolution expression analysis. Valenzuela DM, Murphy AJ, Frendewey D, Gale NW, Economides AN, Auerbach W, Poueymirou WT, Adams NC, Rojas J, Yasenchak J, et al. Nat Biotechnol 2003 21 652 659 10.1038/nbt822 12730667 (Pubitemid 36638092)
10. Conditional knockdown of Fgfr2 in mice using Cre-LoxP induced RNA interference. Coumoul X, Shukla V, Li C, Wang RH, Deng CX, Nucleic Acids Res 2005 33 102 10.1093/nar/gni100 15987787 (Pubitemid 41430597)