Quantification of biotherapeutic targets: New opportunities with immunoaffinity LC-MS/MS

Bioanalysis

Volumn 6, Issue 13, 2014, Pages 1731-1733

  Neubert, Hendrik ^a   Palandra, Joe ^a   Fernández Ocaña, Mireia ^a  

^a PFIZER INC   (United States)

Author keywords

bioanalysis; biotherapeutics; immunoaffinity; LC MS MS; protein targets

Indexed keywords

BINDING PROTEIN; MONOCLONAL ANTIBODY; PEPTIDE ANTIBODY; ANTIBODY; DRUG; LIGAND; PROTEIN;

ANTIBODY AFFINITY; ANTIBODY DETECTION; DRUG DETERMINATION; DRUG TARGETING; LIGAND BINDING; LIQUID CHROMATOGRAPHY; PROCESS OPTIMIZATION; PROTEIN DETERMINATION; PROTEIN TARGETING; QUANTITATIVE ANALYSIS; REVIEW; SENSITIVITY ANALYSIS; TANDEM MASS SPECTROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; IMMUNOAFFINITY CHROMATOGRAPHY; IMMUNOLOGY;

ANTIBODIES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HUMANS; IMMUNOCHROMATOGRAPHY; LIGANDS; PHARMACEUTICAL PREPARATIONS; PROTEINS; TANDEM MASS SPECTROMETRY;

EID: 84906686781     PISSN: 17576180     EISSN: 17576199     Source Type: Journal    
DOI: 10.4155/bio.14.138     Document Type: Review

References (12)

1. Li H, Ortiz R, Tran L et al. General LC-MS/MS method approach to quantify therapeutic monoclonal antibodies using a common whole antibody internal standard with application to preclinical studies. Anal. Chem. 84(3), 1267-1273 (2012).
2. Fernandez Ocana M, James IT, Kabir M et al. Clinical pharmacokinetic assessment of an anti-MAdCAM monoclonal antibody therapeutic by LC-MS/MS. Anal. Chem. 84(14), 5959-5967 (2012).
3. Onami I, Ayabe M, Murao N, Ishigai M. A versatile method for protein-based antigen bioanalysis in non-clinical pharmacokinetics studies of a human monoclonal antibody drug by an immunoaffinity liquid chromatography-tandem mass spectrometry. J. Chromatogr. A 1334(0), 64-71 (2014).
4. Neubert H, Grace C, Rumpel K, James I. Assessing immunogenicity in the presence of excess protein therapeutic using immunoprecipitation and quantitative mass spectrometry. Anal. Chem. 80(18), 6907-6914 (2008).
5. Neubert H, James I. Online capillary weak cation exchange enrichment hyphenated to nanospray mass spectrometry for quantitation of a basic pegvisomant derived peptide. J. Chromatogr. A 1216(33), 6151-6154 (2009).
6. Fernndez Ocaa M, Neubert H. An immunoaffinity liquid chromatography- tandem mass spectrometry assay for the quantitation of matrix metalloproteinase 9 in mouse serum. Anal. Biochem. 399(2), 202-210 (2010).
7. Mcavoy T, Lassman ME, Spellman DS et al. Quantification of tau in cerebrospinal fluid by immunoaffinity enrichment and tandem mass spectrometry. Clin. Chem. 60(4), 683-689 (2014).
8. Neubert H, Muirhead D, Kabir M, Grace C, Cleton A, Arends R. Sequential protein and peptide immunoaffinity capture for mass spectrometry-based quantification of total human beta-nerve growth factor. Anal. Chem. 85(3), 1719-1726 (2013).
9. Palandra J, Finelli A, Zhu M, Masferrer J, Neubert H. Highly specific and sensitive measurements of human and monkey interleukin 21 using sequential protein and tryptic peptide immunoaffinity LC-MS/MS. Anal. Chem. 85(11), 5522-5529 (2013).
10. Agoram BM, Van Der Graaf PH. Biomarkers and biomeasures: key enablers for pharmacokinetic- pharmacodynamic modeling in drug discovery and development. Bioanalysis 4(10), 1143-1145 (2012).
11. Lee J, Kelley M, King L et al. Bioanalytical approaches to quantify total and free therapeutic antibodies and their targets: technical challenges and PK/PD applications over the course of drug development. AAPS J. 13(1), 99-110 (2011).
12. Ackermann BL. Understanding the role of immunoaffinitybased mass spectrometry methods for clinical applications. Clin. Chem. 58(12), 1620-1622 (2012).