Continuous monitoring of enzymatic reactions on surfaces by real-time flow cytometry: Sortase a catalyzed protein immobilization as a case study

Bioconjugate Chemistry

Volumn 25, Issue 8, 2014, Pages 1492-1500

  Heck, Tobias ^a   Pham, Phu Huy ^a   Hammes, Frederik ^b   Thöny Meyer, Linda ^a   Richter, Michael ^a  

^a SWISS FEDERAL LABORATORIES FOR MATERIALS SCIENCE AND TECHNOLOGY   (Switzerland)

^b SWISS FEDERAL INSTITUTE OF AQUATIC SCIENCE AND TECHNOLOGY   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BACTERIA; CATALYSIS; ENZYME IMMOBILIZATION; FLOW CYTOMETRY; SURFACE PLASMON RESONANCE; SURFACE REACTIONS;

CONTINUOUS MONITORING; ENZYMATIC REACTION; MICROBEADS; MICROPARTICLE SURFACES; N-TERMINALS; PROTEIN IMMOBILIZATION; REALTIME FLOWS; SORTASE; SORTASE A; TARGET PROTEINS;

FLUORESCENCE;

AMINO ACID; ENZYME VARIANT; SORTASE; AMINOACYLTRANSFERASE; BACTERIAL PROTEIN; CYSTEINE PROTEINASE; GREEN FLUORESCENT PROTEIN; IMMOBILIZED PROTEIN; POLYSTYRENE DERIVATIVE; SORTASE A;

ARTICLE; COMPARATIVE STUDY; FLOW CYTOMETRY; IMMOBILIZATION; PROTEIN IMMOBILIZATION; QUARTZ CRYSTAL MICROBALANCE; REACTION TIME; SURFACE PLASMON RESONANCE; BIOCATALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; MUTATION; PROTEIN CONFORMATION; STAPHYLOCOCCUS AUREUS; SURFACE PROPERTY; TIME;

AMINOACYLTRANSFERASES; BACTERIAL PROTEINS; BIOCATALYSIS; CYSTEINE ENDOPEPTIDASES; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; IMMOBILIZED PROTEINS; MODELS, MOLECULAR; MUTATION; POLYSTYRENES; PROTEIN CONFORMATION; STAPHYLOCOCCUS AUREUS; SURFACE PROPERTIES; TIME FACTORS;

EID: 84906346778     PISSN: 10431802     EISSN: 15204812     Source Type: Journal    
DOI: 10.1021/bc500230r     Document Type: Article

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