The use of sortase-mediated ligation for the immobilisation of bacterial adhesins onto fluorescence-labelled microspheres: A novel approach to analyse bacterial adhesion to host cells

Biotechnology Letters

Volumn 32, Issue 11, 2010, Pages 1713-1718

  Wu, Si ^a   Proft, Thomas ^a  

^a UNIVERSITY OF AUCKLAND   (New Zealand)

Author keywords

Bacterial adhesin; Fluorescent microspheres; Host cell binding; Sortase mediated ligation

Indexed keywords

ADHESIN; ADHESIVE PROTEINS; BACTERIAL ADHESION; CELL BINDING; CELL SURFACES; CELL WALLS; COLONISATION; COUPLING METHODS; FLUORESCENT MICROSPHERES; GRAM-POSITIVE BACTERIUM; HOST CELLS; IMMOBILISATION; MICROBEADS; PATHOGENIC BACTERIUM; RECOMBINANT PROTEIN; SITE-SPECIFIC; SORTASE; SUBSTRATE SPECIFICITY;

BACTERIA; BIOINFORMATICS; CELL MEMBRANES; ENZYMES; FLUORESCENCE; MICROSPHERES;

BACTERIOLOGY;

ADHESIN; AMINOACYLTRANSFERASE; MICROSPHERE;

ARTICLE; BACTERIUM ADHERENCE; FLUORESCENCE; GRAM POSITIVE BACTERIUM; METABOLISM; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; PHYSIOLOGY; PROTEIN BINDING;

ADHESINS, BACTERIAL; AMINOACYLTRANSFERASES; BACTERIAL ADHESION; BACTERIOLOGICAL TECHNIQUES; FLUORESCENCE; GRAM-POSITIVE BACTERIA; MICROSPHERES; PROTEIN BINDING;

BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 77957963073     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0349-y     Document Type: Article

References (17)

1. Clow F, Fraser JD, Proft T (2008) Immobilization of proteins to biacore sensor chips using Staphylococcus aureus sortase A. Biotechnol Lett 30: 1603-1607.
2. Creti R, Fabretti F, Koch S, Huebner J, Garsin DA, Baldassarri L, Montanaro L, Arciola CR (2009) Surface protein EF3314 contributes to virulence properties of Enterococcus faecalis. Int J Artif Organs 32: 611-620.
3. Hendrickx AP, van Luit-Asbroek M, Schapendonk CM, van Wamel WJ, Braat JC, Wijnands LM, Bonten MJ, Willems RJ (2009) SgrA, a nidogen-binding LPXTG surface adhesin implicated in biofilm formation, and EcbA, a collagen binding MSCRAMM, are two novel adhesins of hospital-acquired Enterococcus faecium. Infect Immun 77: 5097-5106.
4. Krishnan V, Gaspar AH, Ye N, Mandlik A, Ton-That H, Narayana SV (2007) An IgG-like domain in the minor pilin GBS52 of Streptococcus agalactiae mediates lung epithelial cell adhesion. Structure 15: 893-903.
5. Linke C, Young PG, Kang HJ, Bunker RD, Middleditch MJ, Caradoc-Davis TT, Proft T, Baker EN (2010) Crystal structure of the minor pilin FctB reveals determinants of group A Streptococcal pilus anchoring. J Biol Chem. http://www. ncbi. nlm. nih. gov/pubmed/20427291.(in press).
6. Litou ZI, Bagos PG, Tsirigos KD, Liakopoulos TD, Hamodrakas SJ (2008) Prediction of cell wall sorting signals in gram-positive bacteria with a hidden markov model: application to complete genomes. J Bioinform Comput Biol 6: 387-401.
7. Manetti AG, Zingaretti C, Falugi F, Capo S, Bombaci M, Bagnoli F, Gambellini G, Bensi G, Mora M, Edwards AM, Musser JM, Graviss EA, Telford JL, Grandi G, Margarit I (2007) Streptococcus pyogenes pili promote pharyngeal cell adhesion and biofilm formation. Mol Microbiol 64: 968-983.
8. Marraffini LA, Dedent AC, Schneewind O (2006) Sortases and the art of anchoring proteins to the envelopes of gram-positive bacteria. Microbiol Mol Biol Rev 70: 192-221.
9. Navarre WW, Schneewind O (1999) Surface proteins of gram-positive bacteria and mechanisms of their targeting to the cell wall envelope. Microbiol Mol Biol Rev 63: 174-229.
10. Papasergi S, Garibaldi M, Tuscano G, Signorino G, Ricci S, Peppoloni S, Pernice I, Lo Passo C, Teti G, Felici F, Beninati C (2010) Plasminogen- and fibronectin-binding protein B is involved in the adherence of Streptococcus pneumoniae to human epithelial cells. J Biol Chem 285: 7517-7524.
11. Patti JM, Allen BL, McGavin MJ, Hook M (1994) MSCRAMM-mediated adherence of microorganisms to host tissues. Annu Rev Microbiol 48: 585-617.
12. Proft T (2010) Sortase-mediated protein ligation: an emerging biotechnology tool for protein modification and immobilisation. Biotechnol Lett 32: 1-10.
13. Proft T, Arcus VL, Handley V, Baker EN, Fraser JD (2001) Immunological and biochemical characterization of streptococcal pyrogenic exotoxins I and J (SPE-I and SPE-J) from Streptococcus pyogenes. J Immunol 166: 6711-6719.
14. Romero-Steiner S, Caba J, Rajam G, Langley T, Floyd A, Johnson SE, Sampson JS, Carlone GM, Ades E (2006) Adherence of recombinant pneumococcal surface adhesin A (rPsaA)-coated particles to human nasopharyngeal epithelial cells for the evaluation of anti-PsaA functional antibodies. Vaccine 24: 3224-3231.
15. Schneewind O, Mihaylova-Petkov D, Model P (1993) Cell wall sorting signals in surface proteins of gram-positive bacteria. EMBO J 12: 4803-4811.
16. Sillanpaa J, Nallapareddy SR, Qin X, Singh KV, Muzny DM, Kovar CL, Nazareth LV, Gibbs RA, Ferraro MJ, Steckelberg JM, Weinstock GM, Murray BE (2009) A collagen-binding adhesin, Acb, and ten other putative MSCRAMM and pilus family proteins of Streptococcus gallolyticus subsp. gallolyticus (Streptococcus bovis Group, biotype I). J Bacteriol 191: 6643-6653.
17. Terao Y, Kawabata S, Kunitomo E, Murakami J, Nakagawa I, Hamada S (2001) Fba, a novel fibronectin-binding protein from Streptococcus pyogenes, promotes bacterial entry into epithelial cells, and the fba gene is positively transcribed under the Mga regulator. Mol Microbiol 42: 75-86.