Direct site-selective covalent protein immobilization catalyzed by a phosphopantetheinyl transferase

Journal of the American Chemical Society

Volumn 130, Issue 37, 2008, Pages 12456-12464

  Lu, Shin Wong ^a   Thirlway, Jenny ^a   Micklefield, Jason ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; BIOCHIPS; BIOSENSORS; CHEMICAL BONDS; CHEMICAL MODIFICATION; CLUSTERING ALGORITHMS; COLLOIDS; ENZYME ACTIVITY; ENZYMES; FOOD ADDITIVES; FUSION REACTIONS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MICROARRAYS; NUCLEAR PHYSICS; PROTEINS; RESINS; SYSTEM THEORY;

ACYL CARRIER PROTEIN; CELL LYSATE; CHEMICAL-; COVALENT IMMOBILIZATION; COVALENT PROTEIN IMMOBILIZATION; COVALENT-BOND FORMATION; FUNCTIONALIZED; FUSION PROTEINS; GLUTATHIONE S-TRANSFERASE; HOMOGENEOUS MATERIALS; HYDROGEL; LUCIFERASE (LUC); ON METHODS; PHOSPHOPANTETHEINYL TRANSFERASE; PROTEIN MICROARRAYS; RECOMBINANT PROTEINS; RESIN BEADS; SINGLE-STEP; SITE-SELECTIVE; SITE-SPECIFIC; SOLID SUPPORTS; TARGET PROTEINS; THIOREDOXIN FUSION;

CELL IMMOBILIZATION;

ACYL CARRIER PROTEIN; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; LUCIFERASE; RECOMBINANT PROTEIN; RESIN; THIOREDOXIN; TRANSFERASE;

ARTICLE; CATALYSIS; CELL LYSATE; COVALENT BOND; ENZYME ACTIVITY; HYDROGEL; MASS SPECTROMETRY; MICROARRAY ANALYSIS; PROTEIN IMMOBILIZATION; QUANTITATIVE ANALYSIS;

ACYL CARRIER PROTEIN; BACTERIAL PROTEINS; CATALYSIS; COENZYME A; GLUTATHIONE TRANSFERASE; LUCIFERASES, FIREFLY; MODELS, MOLECULAR; PROTEIN ARRAY ANALYSIS; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; TRANSFERASES (OTHER SUBSTITUTED PHOSPHATE GROUPS);

EID: 51949086070     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja8030278     Document Type: Article

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