In vitro Sortagging of an Antibody Fab Fragment: Overcoming Unproductive Reactions of Sortase with Water and Lysine Side Chains

ChemBioChem

Volumn 12, Issue 11, 2011, Pages 1774-1780

  Möhlmann, Sina ^a   Mahlert, Christoph ^a   Greven, Simone ^a   Scholz, Peter ^b   Harrenga, Axel ^a  

^a BAYER PHARMA AG   (Germany)

^b RHINE WAAL UNIVERSITY OF APPLIED SCIENCES   (Germany)

Author keywords

Antibodies; Antitumor agents; Bioconjugation; Protein modifications; Sortase

Indexed keywords

FIBRONECTIN; FLUORESCENT DYE; GLYCINE DERIVATIVE; LYSINE; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY L19 FAB FRAGMENT; SORTASE; UNCLASSIFIED DRUG; WATER;

ARTICLE; CANCER THERAPY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CROSS LINKING; DRUG CONJUGATION; HEAVY CHAIN; HYDROLYSIS; LIGHT CHAIN; PH; PRIORITY JOURNAL; PROTEIN MODIFICATION; SIGNAL TRANSDUCTION;

AMINOACYLTRANSFERASES; BACTERIAL PROTEINS; CYSTEINE ENDOPEPTIDASES; HUMANS; HYDROLYSIS; IMMUNOGLOBULIN FAB FRAGMENTS; LYSINE; MODELS, MOLECULAR; PEPTIDES; SUBSTRATE SPECIFICITY; WATER;

STAPHYLOCOCCUS AUREUS;

EID: 79960483875     PISSN: 14394227     EISSN: 14397633     Source Type: Journal    
DOI: 10.1002/cbic.201100002     Document Type: Article

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