Effect of codon-optimized E. coli signal peptides on recombinant Bacillus stearothermophilus maltogenic amylase periplasmic localization, yield and activity

Journal of Industrial Microbiology and Biotechnology

Volumn 41, Issue 9, 2014, Pages 1435-1442

  Samant, Shalaka ^a   Gupta, Gunja ^a,b   Karthikeyan, Subbulakshmi ^a,c   Haq, Saiful F ^a   Nair, Ayyappan ^a   Sambasivam, Ganesh ^a   Sukumaran, Sunilkumar ^a  

^a Anthem Biosciences Pvt Ltd   (India)

^b APOLLO HOSPITALS   (India)

^c UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

Codon optimization; E. coli; Maltogenic amylase; Periplasmic localization; Signal peptides

Indexed keywords

AMYLASE; MALTOGENIC AMYLASE; SIGNAL PEPTIDE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CODON; GLUCAN 1,4-ALPHA-MALTOHYDROLASE; GLYCOSIDASE; HYBRID PROTEIN;

ARTICLE; CODON; CODON USAGE; CYTOPLASM; ENZYME ACTIVITY; ENZYME LOCALIZATION; ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS; NONHUMAN; PROTEIN SECRETION; PROTEIN STABILITY; SEQUENCE ANALYSIS; SIGNAL TRANSDUCTION; ENZYMOLOGY; GENE EXPRESSION; GENETICS; METABOLISM; PROTEIN ENGINEERING; PROTEIN TRANSPORT;

ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS;

BACTERIAL PROTEINS; CODON; ESCHERICHIA COLI; GENE EXPRESSION; GEOBACILLUS STEAROTHERMOPHILUS; GLYCOSIDE HYDROLASES; PERIPLASM; PROTEIN ENGINEERING; PROTEIN SORTING SIGNALS; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS;

EID: 84906262131     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-014-1482-8     Document Type: Article

References (26)

1. Angelini S, Moreno R, Gouffi K, Santini C, Yamagishi A, Berenguer J, Wu L (2001) Export of Thermus thermophilus alkaline phosphatase via the twin-arginine translocation pathway in Escherichia coli. FEBS Lett 506:103-107 (Pubitemid 32925208)
2. Baneyx F, Muhacic M (2004) Recombinant protein folding and misfolding in Escherichia coli. Nat Biotechnol 22:1399-1408
3. Cha HJ, Yoon HG, Kim YW, Lee HS, Kim JW, Kweon KS, Oh BH, Park KH (1998) Molecular and enzymatic characterization of a maltogenic amylase that hydrolyzes and transglycosylates acarbose. Eur J Biochem 253:251-262
4. Choi JH, Jeong KJ, Kim SC, Lee SY (2000) Efficient secretory production of alkaline phosphatase by high cell density culture of recombinant Escherichia coli using the Bacillus sp. endoxylanase signal sequence. Appl Microbiol Biotechnol 53:640-645 (Pubitemid 30430216)
5. Choi JH, Lee SY (2004) Secretory and extracellular production of recombinant proteins using Escherichia coli. Appl Microbiol Biotechnol 64:625-635 (Pubitemid 38822625)
6. Diderichsen et al. (1986) Preparation of a maltogenic amylase enzyme United States Patent 4598048
7. Fekkes P, Driessen AJ (1999) Protein targeting to the bacterial cytoplasmic membrane. Microbiol Mol Biol Rev 63:161-173 (Pubitemid 29116091)
8. Georgiou G, Segatori L (2005) Preparative expression of secreted proteins in bacteria: status report and future prospects. Curr Opin Biotechnol 5:538-545 (Pubitemid 41393835)
9. Gold L (1990) Expression of heterologous proteins in Escherichia coli. Method Enzymol 185:11-14
10. Izard JW, Kendall DA (1994) Signal peptides: exquisitely designed transport promoters. Mol Microbiol 13:765-773 (Pubitemid 24272197)
11. Lee SY, Jeong KJ (2006) US20067070989B2
12. Makrides SC (1996) Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol Rev 60:512-538
13. Marino MH (1989) Expression systems for heterologous protein production. BioPharmacology 2:18-33
14. Mergulhão FJ, Summers DK, Monteiro GA (2005) Recombinant protein secretion in Escherichia coli. Biotechnol Adv 23:177-202
15. Olins PO, Lee SC (1993) Recent advances in heterologous gene expression in Escherichia coli. Curr Opin Biotechnol 4:520-525 (Pubitemid 23300184)
16. Pugsley AP (1993) The complete general secretory pathway in gram-negative bacteria. Microbiol Rev 57:50-108
17. Qian ZG, Xia XX, Choi JH, Lee SY (2008) Proteome-based identification of fusion partner for high-level extracellular production of recombinant proteins in Escherichia coli. Biotechnol Bioeng 101:587-601
18. Rouet R, Lowe D, Dudgeon K, Roome B, Schofield P, Langley D, Andrews J, Whitfeld P, Jermutus L, Christ D (2012) Expression of high-affinity human antibody fragments in bacteria. Nat Protoc 7:364-373
19. Sargent F, Gohlke U, de Leeuw E, Stanley NR, Palmer T, Saibil HR, Berks BC (2001) Purified components of the Escherichia coli Tat protein transport system form a double layered ring structure. Eur J Biochem 268:3361-3367 (Pubitemid 32862927)
20. Shokri A, Sanden AM, Larsson G (2003) Cell and process design for targeting of recombinant protein. Appl Microbiol Biotechnol 60:654-664
21. Singh P, Sharma L, Kulothangan RS, Adkar BV, Prajapati RS et al (2013) Effect of signal peptide on stability and folding of Escherichia coli thioredoxin. PLoS ONE 8(5):e63442
22. Stuart RA, Neupert W (2000) Making membranes in bacteria. Nature 406:575-577 (Pubitemid 30641029)
23. Valent QA, Scotti PA, High S, de Gier JW, von Hejine G, Lentzen G, Wintermeyer W, Oudega B, Luirink J (1998) The Escherichia coli SRP and SecB targeting pathways converge at the translocon. EMBO J 17(9):2504-2512 (Pubitemid 28221186)
24. Zalucki YM, Beachem IR, Jennings MP (2009) Biased codon usage in signal peptides: a role in protein export. Trends Microbiol 17:146-150
25. Zalucki YM, Jones CE, Ng PS, Schulz BL, Jennings MP (2010) Signal sequence non-optimal codons are required for the correct folding of mature maltose binding protein. Biochem Biophys Acta 1798:1244-1249
26. Zalucki YM, Shafer WM, Jennings MP (2011) Directed evolution of efficient secretion in the SRP-dependent export of TolB. Biochem Biophys Acta 1808:2544-2550