Overexpression and biochemical characterization of a thermostable phytase from Bacillus subtilis US417 in Pichia pastoris

Molecular Biotechnology

Volumn 56, Issue 9, 2014, Pages 839-848

  Hmida Sayari, Aïda ^a   Elgharbi, Fatma ^a   Farhat, Ameny ^a   Rekik, Hatem ^a   Blondeau, Karine ^b   Bejar, Samir ^a  

^a CENTRE DE BIOTECHNOLOGIE DE SFAX   (Tunisia)

^b UNIVERSITÉ PARIS SACLAY   (France)

Author keywords

Fermentation; Heterologous expression; Phytase; Pichia pastoris

Indexed keywords

FERMENTATION; GENE ENCODING; PURIFICATION; YEAST;

BACILLUS SUBTILIS US417; BIOCHEMICAL CHARACTERIZATION; HETEROLOGOUS EXPRESSION; METHANOL-INDUCIBLE; PHYTASES; PICHIA PASTORIS; RECOMBINANT STRAINS; SEQUENCE ENCODING;

ENZYMES;

ALCOHOL OXIDASE; GLYCOSYLATED PROTEIN; PHYTASE; BACTERIAL PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL STRAIN; BACTERIUM COLONY; CELL DENSITY; CONTROLLED STUDY; DEGLYCOSYLATION; ENZYME ACTIVITY; ENZYME ANALYSIS; FED BATCH CULTURE; GENE OVEREXPRESSION; GENE SEQUENCE; MOLECULAR WEIGHT; NONHUMAN; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE; THERMOSTABILITY; BIOSYNTHESIS; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; GLYCOSYLATION; METABOLISM; MOLECULAR CLONING; PICHIA; PROMOTER REGION;

BACILLUS SUBTILIS; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

6-PHYTASE; BACILLUS SUBTILIS; BACTERIAL PROTEINS; CLONING, MOLECULAR; ENZYME STABILITY; GLYCOSYLATION; PICHIA; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS;

EID: 84905974091     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-014-9764-y     Document Type: Article

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