Expression, purification, crystallization and preliminary X-ray diffraction analysis of a mammalian type 10 adenylyl cyclase

Acta Crystallographica Section F:Structural Biology Communications

Volumn 70, Issue 4, 2014, Pages 467-469

  Kleinboelting, Silke ^a   Van Den Heuvel, Joop ^b   Kambach, Christian ^a   Weyand, Michael ^a   Leipelt, Martina ^c   Steegborn, Clemens ^a  

^a UNIVERSITY OF BAYREUTH   (Germany)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^c RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

catalytic core; mammalian; soluble adenylyl cyclase; type 10

Indexed keywords

ADENYLATE CYCLASE; RECOMBINANT PROTEIN;

BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; CRYSTALLIZATION; ENZYME ACTIVE SITE; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PROCEDURES; PROTEIN CONFORMATION; X RAY CRYSTALLOGRAPHY;

ADENYLATE CYCLASE; BINDING SITES; CATALYTIC DOMAIN; CLONING, MOLECULAR; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; HUMANS; MODELS, MOLECULAR; PROTEIN CONFORMATION; RECOMBINANT PROTEINS;

EID: 84905463613     PISSN: None     EISSN: 2053230X     Source Type: Journal    
DOI: 10.1107/S2053230X14004014     Document Type: Article

References (23)

1. Acin-Perez, R., Russwurm, M., Günnewig, K., Gertz, M., Zoidl, G., Ramos, L., Buck, J., Levin, L. R., Rassow, J., Manfredi, G. & Steegborn, C. (2011). J. Biol. Chem. 286, 30423-30432.
2. Chaloupka, J. A., Bullock, S. A., Iourgenko, V., Levin, L. R. & Buck, J. (2006). Mol. Reprod. Dev. 73, 361-368.
3. Chen, Y., Cann, M. J., Litvin, T. N., Iourgenko, V., Sinclair, M. L., Levin, L. R. & Buck, J. (2000). Science, 289, 625-628.
4. Gertz, M., Fischer, F., Wolters, D. & Steegborn, C. (2008). Proc. Natl Acad. Sci. USA, 105, 5705-5709.
5. Hall, R. A., De Sordi, L., Maccallum, D. M., Topal, H., Eaton, R., Bloor, J. W., Robinson, G. K., Levin, L. R., Buck, J., Wang, Y., Gow, N. A., Steegborn, C. & Mühlschlegel, F. A. (2010). PLoS Pathog. 6, e1001193.
6. Hanoune, J. & Defer, N. (2001). Annu. Rev. Pharmacol. Toxicol. 41, 145-174.
7. Hess, K. C., Jones, B. H., Marquez, B., Chen, Y., Ord, T. S., Kamenetsky, M., Miyamoto, C., Zippin, J. H., Kopf, G. S., Suarez, S. S., Levin, L. R., Williams, C. J., Buck, J. & Moss, S. B. (2005). Dev. Cell, 9, 249-259.
8. Kabsch, W. (2010). Acta Cryst. D66, 125-132.
9. Kamenetsky, M., Middelhaufe, S., Bank, E. M., Levin, L. R., Buck, J. & Steegborn, C. (2006). J. Mol. Biol. 362, 623-639.
10. Linder, J. U. (2006). Cell. Mol. Life Sci. 63, 1736-1751.
11. Litvin, T. N. (2004). PhD thesis, Cornell Medical College, New York City, USA.
12. Matthews, B. W. (1968). J. Mol. Biol. 33, 491-497.
13. Middelhaufe, S., Leipelt, M., Levin, L. R., Buck, J. & Steegborn, C. (2012). Biosci. Rep. 32, 491-499.
14. Mueller, U., Darowski, N., Fuchs, M. R., Förster, R., Hellmig, M., Paithankar, K. S., Pühringer, S., Steffien, M., Zocher, G. & Weiss, M. S. (2012). J. Synchrotron Rad. 19, 442-449.
15. Rauch, A., Leipelt, M., Russwurm, M. & Steegborn, C. (2008). Proc. Natl Acad. Sci. USA, 105, 15720-15725.
16. Schlicker, C., Rauch, A., Hess, K. C., Kachholz, B., Levin, L. R., Buck, J. & Steegborn, C. (2008). J. Med. Chem. 51, 4456-4464.
17. Sinha, S. C. & Sprang, S. R. (2006). Rev. Physiol. Biochem. Pharmacol. 157, 105-140.
18. Steegborn, C., Litvin, T. N., Hess, K. C., Capper, A. B., Taussig, R., Buck, J., Levin, L. R. & Wu, H. (2005). J. Biol. Chem. 280, 31754-31759.
19. Steegborn, C., Litvin, T. N., Levin, L. R., Buck, J. & Wu, H. (2005). Nature Struct. Mol. Biol. 12, 32-37.
20. Sunahara, R. K. & Taussig, R. (2002). Mol. Interv. 2, 168-184.
21. Tesmer, J. J., Sunahara, R. K., Gilman, A. G. & Sprang, S. R. (1997). Science, 278, 1907-1916.
22. Wasilko, D. J., Lee, S. E., Stutzman-Engwall, K. J., Reitz, B. A., Emmons, T. L., Mathis, K. J., Bienkowski, M. J., Tomasselli, A. G. & Fischer, H. D. (2009). Protein Expr. Purif. 65, 122-132.
23. Zippin, J. H., Farrell, J., Huron, D., Kamenetsky, M., Hess, K. C., Fischman, D. A., Levin, L. R. & Buck, J. (2004). J. Cell Biol. 164, 527-534.