The mechanisms of in vitro storage organ formation in ornamental geophytes

Floriculture and Ornamental Biotechnology

Volumn 6, Issue SPEC.ISS.1, 2012, Pages 9-23

  Podwyszyńska, Matgorzata ^a  

^a RESEARCH INSTITUTE OF HORTICULTURE   (Poland)

Author keywords

Abscisic acid; Auxin; Bulbs; Corms; Cytokinins; Geophytes; Gibberellin; Growth retardants; In vitro; Jasmonate; Polyamines; Tuberous roots; Tubers

Indexed keywords

EID: 84905104537     PISSN: 17490294     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (161)

1. Abelenda JA, Navarro C, Prat S (2011) From the model to the crop: Genes controlling tuber formation in potato. Current Opinion in Biotechnology 22, 287-292
2. Addai IK (2010) Growth and biochemistry of the common hyacinth (Hya-cinthus oriental is L.) and the lily (Lilium longiflortim L.). PhD thesis, University of Sussex, UK, 285 pp
3. Aksenova NP, Konstantinova TN, Golyanovskaya SA, Sergeeva LI, Romanov GA (2012) Hormonal regulation of tuber formation in potato plants. Russian Journal of Plant Physiology 59,451-466
4. Appeldoorn NJG, Sergeeva L, Vrcugdenhil D, Van Der Plas LHW, Visser RGF (2002) In situ analysis of enzyme involved in sucrose to hexose-phos-phate conversion during stolon-to-tuber transition of potato. Physiologia PlantarumUS, 303-310
5. Ascough GD, Erwin JE, Van Staden J (2007) In vitro propagation of four Wat-sonia species. Plant Cell, Tissue and Organ Culture 88, 135-145
6. Ascough GD, Erwin JE, Van Staden J (2008a) In vitro storage organ formation on ornamental geophytes. Horticultural Reviews 34,417-444
7. Ascough GD, Erwin JE, Van Staden J (2008b) Reduced temperature, elevated sucrose, continuous light and gibberellic acid promote corm formation in Watsonia vanderspuyiae. Plant Cell, Tissue and Organ Culture 95, 275-284
8. Ascough GD, Erwin JE, Van Staden J (2009) Micropropagation of Iridaceae-a review. Plant Cell, Tissue and Organ Culture 97, 1-19
9. Ascough GD, Swart PA, Finnie JF, Van Staden J (2011) Micropropagation of Romulea minutiflora, Sisyrinchium laxum and Tritonia gladiolaris-Iridaceae with ornamental potential. South African Journal of Botany 77, 216-221
10. Azadi P and Khosh-Khul M (2007) Micropropagation of Lilium ledebourii (Baker) Boiss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatment. Electronic Journal of Biotechnology 10, 582-591
11. Bach A, Pawlowska B, Pulczynska K (1992) Utilization of soluble carbohydrates in shoot and bulb regeneration of Hyacinthus orientalis L. in vitro. Acta Horticulturae 325,487-491
12. Cenzano A, Vigliocco A, Kraus T, Abdala G (2003) Exogenously applied jas-monic acid induces changes in apical meristem morphology of potato stolons. Annals of Botany 91,915-919
13. Chanteloube F, Tort M, Courduroux CJ, Le Nard M (1993) Production de bulbes in vitro et de novo a partir de tissues de tiges feuillees floriferes de Tulipa gesnerian L, cv Lucky Strike en cours ddongation: Etude histolo-gique. Agronomie 13, 179-188
14. Chen J, Ziv M (2005) The effect of storage condition on starch metabolism and regeneration potentials of twin-scales and inflorescence stem explants of Narcissus tazetta. In Vitro Cellular and Developmental Biology-Plant 41,816-821
15. Chung KH, Nam SI (2001) Method for producing microbulbs of garlic (Allium sativum L). US Patent 6265217
16. Dantu PK, Bhojwani SS (1995) In vitro corm formation and field evaluation of corm-derived plants of Gladiolus. Scientia Horticulturae 61,115-129
17. De Bruyn MH, Ferreira DI (1992) In vitro corm production of Gladiolus dalenii and G tristis. Plant Cell, Tissue and Organ Culture 31,123-128
18. De Hertogh A, Le Nard M (1993) The Physiology of Flower Bulb, Elsevier, Amsterdam, 812 pp
19. De Klerk GJ (2012) Micropropagation of bulbous crops: Technology and present state. In: Van Tuyl J, Arens P (Eds) Bulbous Ornamentals (Bulbs, Cor-mels and Tubers). Floriculture and Ornamental Biotechnology 6 (Special Issue 1), 1-8
20. Dobranszkt J, Magyar-Tabori K, Hudak I (2008) In vitro tuberization in hormone-free system on solidified medium and dormancy of potato micro-tubers. In: Benkeblia N, Tennant P (Eds) Potato I. Fruit, Vegetable and Cereals Science and Biotechnology 2 (Special Issue 1), 82-94
21. Donnely DJ, Coleman WK, Coleman SE (2003) Potato microtuber production and performance: A review. American Journal of Potato Research 80, 103-115
22. Du Y, Li W, Zhang M, He H, Jia G (2012) The establishment of a slow-growth conservation system in vitro for two wild lily species. African Journal of Biotechnology 11,1981-1990
23. Forsyth C, Van Staden J (1984) Tuberization of Dioscorea bulbifera stem nodes in culture. Journal of Plant Physiology 115,78-83
24. Fujino K, Koda Y, Kikuta Y (1995) Reorientation of cortical microtubules in the sub-apical region during tuberization in single-node stem segments of potato in culture. Plant and Cell Physiology 36,891-895
25. Gabryszewska E (2010) The effect of glucose and growth regulators on the organogenesis of Paeonia lactiflora Pall in vitro. Journal of Fruit and Ornamental Plant Research 18, 1-12
26. Gerrits MM, Kim KS, De Klerk GJ (1992) Hormonal control of dormancy in bulblets Lilium speciosum cultured in vitro. Acta Horticulturae 325, 521-527
27. Halevy AH, Biran I (1975) Hormonal regulation of tuberization in Dahlia. Acta Horticulturae 47,319-330
28. He X, Shi L, Yuan Z, Xu Z, Zhang Z, YI M (2008) Effect of lipoxygenase on the corm formation and enlargement in Gladiolus hybridus. Scientia Horticulturae 118,60-69
29. Hong J, Lee AK (2012) Micropropagation of Cyrtanthus Orange Gem x C. eucallus hybrid. Scientia Horticulturae 142,174-179
30. Hussey G (1982) In vitro propagation of Narcissus. Annals of Botany 49, 707-719
31. Hyndman SE, Hasegawa PM, Brcssan RA (1982) The role of sucrose and nitrogen in adventitious root formation on cultured rose shoots. Plant Cell, Tissue and Organ Culture 1, 229-238
32. Ilan A, Ziv M, Halevy H (1995) Propagation and corm development of Bro-diaea in liquid cultures. Scientia Horticulturae 63, 101-112
33. Ilczuk A, Winkelmann T, Richartz S, Witomska M, Serek M (2005) In vitro propagation of Hippeastrum x chmielii Chm.-influence of fiurprimidol and the culture in solid or liquid medium and in temporary immersion systems. Plant Cell, Tissue and Organ Culture 83, 339-346
34. Ishimori T, Nilmi Y, Han D-S (2007) Benzyladenine and low temperature promote phase transition from juvenile to vegetative adult in bulblets of Lilium x formolongi White Aga cultured in vitro. Plant Cell, Tissue and Organ Culture 88,313-318
35. Jackson SD (1999) Multiple signaling pathways control tuber induction in potato. Plant Physiology 119, 1-8
36. Jasik J, De Klerk GJ (2006) Effect of methyl jasmonate on morphology and dormancy development in lily bulblets generated in vitro. Journal of Plant Growth Regulation 25,45-51
37. Jin M (2009) Effects of methyl jasmonate (MeJA) on bulblet and scale leaf growth of Lilium Mona in vitro. Journal of Anhui Agricultural Sciences 1 (Abstract)
38. Kamerbeek GA, Beijersbergen JCM, Schenk PK (1970) Dormancy in bulbs and corms. Proceedings of 18th International Congress, Tel Aviv 5, 233-240
39. Karaguzel O, Kaya A, Biner B, Aydinsaldr K (2012) In vitro propagation of native Omilhogalum species in West Mediterranean region of Turkey. African Journal of Agricultural Research 7, 2669-2673
40. Kaur-Sawhney R, Tiburcio AF, Altabella T, Galston AW (2003) Poly-amines in plants: An overview. Journal of Cell Molecular Biology 2, 81-85
41. Kawa-Mlszczak L, Wȩgrzynowicz E, Saniewski M (1992) The effect of the removal of roots and application of plant growth regulation on tulip shoot growth. Acta Horticulturae 325, 71-76
42. Keller ERJ (1993) Sucrose, cytokinin, and ethylene influence formation of in vitro bulblets in onion and leek. Genetic Resources and Crop Evolution 40, 113-120
43. Kim EK, Hahn EJ, Murthy HN, Paek KY (2003) High frequency of shoot multiplication and bulblet formation of garlic in liquid cultures. Plant Cell, Tissue and Organ Culture 73,231-236
44. Kim KS, Davelaar E, De Klerk GJ (1994) Abscisic acid controls dormancy development and bulb formation in lily plantlets regenerated in vitro. Physiologia Plantarum 90,59-64
45. Koch KE (1996) Carbohydrate regulated gene expression in plants. Annual Review of Plant Physiology and Plant Molecular Biology 47,509-540
46. Koda Y (1997) Possible involvement of jasmonates in various morphogenic events. Physiologia Plantarum 100,639-646
47. Koda Y, Okazawa Y (1983) Influences of environmental, hormonal and nutritional factors on potato tuberization in vitro. Japanese Journal of Crop Science SI, 582-591
48. Koetle MJ, Finnie JF, Van Staden J (2010) In vitro regeneration in Dierama erectum Hillary. Plant Cell, Tissue and Organ Culture 103,23-31
49. Kozak D (2002) The effect of growth retardants on induction and developments of Gloriosa rothschildiana OBrien tubers in vitro. Acta Horticulturae 570, 345-349
50. Kozak D (2003) Effect of medium components on in vitro tuberization of Gloriosa rothschildiana OBrien. Acta Horticulturae 624,515-520
51. Krauss A (1985) Interaction of nitrogen nutrition, phytohormones and tuberization. In: Li PH (Ed) Potato Physiology, Academic Press, London, pp 209-231
52. Kuijpers AM, Langens-Gerrits M (1997) Propagation of tulip in vitro. Acta Horticulturae 430,321-324
53. Kumar A, Palni LMS (2011) Factors affecting in vitro formation of cormlets in Gladiolus hybridus Hort. and their field performance. Acta Physiologiae Plantarum 33, 509-515
54. Kumar A, Taylor MA, Mad Arif SA, Davies HV (1996) Potato plants expressing antisense and sense S-adenosylmethionine decarboxylase (SAMDC) transgenes show altered levels of polyamines and ethylene: Antisense plants display abnormal phenotypes. Plant Journal 9,147-158
55. Kumar S, Kashyap M, Sharma DR (2005) In vitro regeneration and bulblet growth from lily bulbscale explants as affected by retardants, sucrose and irradiance. Biologia Plantarum 49,629-632
56. Langens-Gerrits MM, Miller WBM, Croes AF, De Klerk GJ (2003) Effect of low temperature on dormancy and growth after planting in lily bulblets regenerated in vitro. Plant Growth Regulation 40, 267-275
57. Le Guen-Le Saos F, Hourmant A, Esnault F, Chauvin JE (2002) In vitro bulb development in shallot (Allium cepa L. Aggregatum Group): Effects of anti-gibberellins, sucrose and light. Annals of Botany 89,419-425
58. Le Nard M, De Hertogh AA (1993) Tulipa. In: De Hertogh AA, Le Nard M (Eds) The Physiology of Flower Bulb, Elsevier Science Publishers, The Netherlands, pp 617-682
59. Le Nard M, Ducommun C, Weber G, Dorion N, Bigot C (1987) Observations sur la multiplication in vitro de la tulipe (Tulipa gesneriana L.) a partir de hampes florales prelevees chez des bulbes en cours de conservation. Agronomie 7, 321-329
60. Li K, Okubo H, Matsumoto T (2002) Control of bulb dormancy in hyacinth-a molecular biological approach. Acta Horticulturae 570,241-246
61. Li-Nagasuga K, Okubo H (2005) Characterization of genes related to hyacinth bulb formation induced by low temperature or ABA. Acta Horticulturae 673,575-582
62. Loebenstein G, Lawson RH, Brunt AA (1995) Virus and Virus-Like Diseases of Bulb and Flower Crop, John Wiley and Sons, Chichester, UK, 350 pp
63. Mader JC (1999) Effect of jasmonic acid, silver nitrate and L-AOPP on the distribution of free and conjugated polyamines in roots and shoots of Sola-num tuberosum in vitro. Journal of Plant Physiology 154, 79-88
64. Madubanya LA, Makunga NP, Fennell CW (2006) Dierama luteoalbidum: Liquid culture provides an efficient system for the ex situ conservation of an endangered and hotticulturally valuable plant. South African Journal of Botany 72, 584-588
65. Maehara, K, Li-Nagasuga K, Okubo H (2005) Search for genes regulating bulb formation in Lilium speciosum by differential display. Acta Horticulturae 673,583-589
66. Malkawi A, Jensen BL, Langille AL (2007) Plant hormones isolated from "Katahdin" potato plant tissues and the influence of photoperiod and temperature on their levels in relation to tuber induction. Journal of Plant Growth Regulation 26, 308-317
67. Medina RD, Falocl MM, Gonzales AM, Mroglnski LA (2007) In vitro cultured primary roots derived from stem segments of cassava (Manihot escu-lenla) can behave like storage organs. Annals of Botany 99,409-423
68. Miller WB (1992) A review of carbohydrate metabolism in geophytes. Acta Horticulturae 325,239-246
69. Mohammadi-Dehcheshmeh M, Khalighi A, Naderi R, Sardarii M, Ebrahi-mie E (2008) Petal a reliable explant for direct bulblet regeneration of endangered wild populations of Fritillaria imperialis L. Acta Physiologiae Plantarum 30,395-399
70. Mok DWS, Mok MC (2001) Cytokinins metabolism and action. Annual Review of Plant Physiology 52,89-118
71. Moyo M, Bairu MW, Amoo SO, Van Staden J (2011) Plant biotechnology in South Africa: Micropropagation research endeavours, prospects and challenges. South African Journal of Botany 77,996-1011
72. Murashlge T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum 15,473-97
73. Nagaraju V, Bhowmik G, Parthasarathy VA (2002) Effect of paclobutrazol and sucrose on in vitro cormel formation in gladiolus. Acta Botanica Croa-tica 61,27-33
74. Naik PK, Nayak S (2005) Different modes of plant regeneration and factors affecting in vitro bulblet production in Omithogalum virens. Science Asia 31, 409-414
75. Nam K-H, Mlnaml C, Kong F, Matsuura H, Takahashi K, Yoshihara T (2007) Relation between environmental factors and the LOX activities upon potato tuber formation and flowering-bud formation in morning glory. Plant Growth Regulation 46,253-260
76. Naslrcilar AG, Mirici S, Karaguzel O, Eren O, Baktir 1 (2011) In vitro propagation of endemic and endangered Muscari mirum from different explant types. Turkish Journal of Botany 35,37-43
77. Nhut DT (1998) Micropropagation of lily (Lilium longiflorum) via in vitro stem node and pseudo-bulblet culture. Plant Cell Reports 17,913-916
78. Nhut DT, Teixeira da Silva JA, Huyen PX, Paek KY (2004) The importance of explant source on regeneration and micropropagation of Gladiolus by liquid shake culture. Stientia Horticulturae 102,407-414
79. Niimi Y, Onozawa T (1979) In vitro bulblet formation from leaf segments of lilies especially Lilium rubellum Baker. Scientia Horticulturae 11, 379-389
80. Nishiuchi Y (1980) Studies on vegetative propagation of tulip. 4. Regeneration of bulblets in bulb scale segments cultured in vitro. Journal of the Japanese Society for Horticultural Science 49, 235-239
81. Nojiri H, Toyomasu T, Yamane H, Shibaoka H, Murofushl N (1993) Qualitative and quantitative analysis of endogenous gibberellins in onion (Allium cepa) plants and their effects on bulb development. Bioscience, Biotechnology, and Biochemistry 57,2031-2035
82. Nojiri H, Yamane H, Seto H, Yamaguchi I, Murofushl N, Yoshihara T, Shibaoka H (1992) Qualitative and quantitative analysis of endogenous jas-monic acid in bulbing and non-bulbing onion plants. Plant and Cell Physiol-ogy 33,1225-1231
83. Ohyama T, Komiyama S, Ohtake N, Sueyoshi K, Teixeira da Silva JA, Ruamrungsri S (2006) Physiology and genetics of carbon and nitrogen metabolism in tulip. In: Teixeira da Silva JA (Ed) Floriculture, Ornamental and Plant Biotechnology, Advances and Topical Issues (Vol 111), Global Science Books, Isleworth, UK, pp 12-25
84. Okubo H (2000) Growth cycle and dormancy in plants. In: Viemont J-D, Crab-ble J (Eds) Dormancy in Plants: From Whole Plant Behaviour to Cellular Control, CABI Publishing Wallingford, Oxon, pp 1-22
85. Omokolo ND, Boudjeko T, Tsafack Takadong JJ (2003) In vitro tuberization of Xanthosoma sagittifolium L. Schott: Effect of phytohormones, sucrose, nitrogen and photoperiod. Scientia Horticulturae 98,333-345
86. Ovono PO, Kevers C, Dommes J (2007) Axillary proliferation and tuberization of Dioscorea cayenensis-D. rotundata complex. Plant Cell. Tissue and Organ Culture 91, 107-114
87. Ovono PO, Kevers C, Dommes J (2010) Tuber formation and development of Dioscorea cayenensis-Dioscorea rotundata complex in vitro effect of poly-amines. In Vitro Cellular and Developmental Biology-Plant 46,81-88
88. Park J-Y, Kim H-S, Youm J-W, Kim M-S, Kim K-S, Joung H, Jeon J-H (2006) Cloning of superoxide dismutase (SOD) gene of lily "Marcopolo and expression in transgenic potato. Agricultural Chemistry and Biotechnology 49, 1-7
89. Pedros AR, Ross HA, McRae D, Tiburclo AF, Davles HV, Taylor MA (1999) Manipulation of S-adenosylmethionine decarboxylase activity in potato tubers. An increase in activity leads to an increase in tuber number and a change in tuber size distribution. Planta 209,153-160
90. Pierik RLM, Steegmans HHM (1975) Effect of auxins, cytokinins, gibberellins, abscisic acid and ethephon on regeneration and growth of bulblets on excised bulb scale segments of hyacinth. Physiologia Plantarum 34, 14-17
91. Pina-Escuria JL, Vasquez-Garcia LM, Arzate-Feznandez AM (2010) In vitro regeneration and genetic fidelity of Tigridia (L.f.) DC. Electronic Journal of Biotechnology 13,1-7
92. Podwyszynska M (2001) Effect of carbohydrates on shoot multiplication and bulb formation of tulip in vitro. Roczniki Akademii Rolniczej w Poznaniu, Ogrodnictwo 33,119-126
93. Podwyszynska M (2005) Somaclonal variation in micropropagated tulips based on phenotype observation. Journal of Fruit and Ornamental Plant Research 13, 109-122
94. Podwyszynska M (2006a) Effect of ethylene, auxin and methyl jasmonate on bulb formation in vitro in tulip shoot cultures. Advances of Agricultural Sciences Problem Issues 510,461-469 (in Polish with English abstract)
95. Podwyszynska M (2006b) Improvement of bulb formation in micropropagated tulips by treatment with NAA and paclobutrazol or ancymidol. Acta Horticulturae 725, 679-684
96. Podwyszynska M, Michalczuk L, Miszczak A (2004) Content of endogenous abscisic acid in tulip shoots cultured in vitro depending on micro-propagation stage and genotype. Abstracts of IX International Symposium on Flower Bulbs, Niigata, Japan, 19-22.04.2004, 122 pp
97. Podwyszynska M, Novak O, Strnad M, Saniewski M (2005) Endogenous cytokinins in micropropagated tulips. In: Book of Abstract of 6th International Symposium in the Series "Recent Advance un Plant Biotechnology", September 12-16,2005, Ceske Budejovice, Czech Republic, 126 pp
98. Podwyszynska M, Ross H (2003) Formation of tulips bulbs in vitro. Acta Horticulturae 616,413 A17
99. Podwyszynska M, Sochacld D (2010) Micropropagation of tulip: produc-tion of virus-free stock plants. In: Jain SM, Ochatt SJ (Eds) Protocols for in Vitro Propagation of Ornamental Plants. Methods in Molecular Biology (Springer Protocols) 589, Humana Press/Springer, New York, US, pp 243-256
100. Prado MC, Rodríguez MA, Monter AV, Tejacal IA, Torres OGV, Martf-nez VL (2012) In vitro propagation of Sprekelia formosissima Herbert., a wild plant with ornamental potential. Revista Fitotecnia Mexicana 33, 197-203
101. Raja W, Zaffer G, Wanl SA (2007) In vitro microcorm formation in saffron (Crocus sativus L.). Acta Horticulturae 739,291-296
102. Ranwala AP, Miller WB (2008) Analysis of nonstructural carbohydrates in storage organs of 30 ornamental geophytes by high-performance anion-exchange chromatography with pulsed amperometric detection. New Phy-tologist 180,421-433
103. Ravnikar M, Zel J, Plaper i, Spacapan A (1993) Jasmonic acid stimulates shoot and bulbformation of garlic in vitro. Journal of Plant Growth Regulation 12,73-77
104. Rebers M, Vermeer E, Knegt E, Shelton CJ, Van Der Plas LHW (1995) Gibberellin levels and cold-induced floral stalk elongation in tulip. Physiologia Plantarum 94,687-691
105. Rees AR (1992) Ornamental Bulbs. Corms and Tubers, CAB International, Oxon, UK, 570 pp
106. Rice U, Finnie JF, Van Staden J (2011) In vitro bulblet formation of Bruns-vigia undulata from twin-scales. South African Journal of Botany 77, 305-312
107. Rice RD, Alderson PG, Wright NA (1983) Induction of bulbing of tulip shoots in vitro. Scientia Horticulturae 20, 377-390
108. Rietveld PL, Wilkinson C, Franssen HM, Balk PA, Van der Plas LHW, Weisbeek PJ, de Boer AD (2000) Low temperature sensing in tulip (Tulipa gesneriana L.) is mediated through an increased response to auxin. Journal of Experimental Botany 51, 587-594
109. Riou-Khamlichi C, Hunley R, Jacqmard A, Murray JA (1999) Cytokinin activation of Arabidopsis cell division through a D-type cyclin. Science 283, 1541-1544
110. Rodrigues-Falcón M, Bou J, Prat S (2006) Seasonal control of tuberization in potato: Conserved elements with the flowering response. Annual Review of Plant Biology 57, 151-180
111. Rohwer CL, Erwin JE (2008) Horticultural applications of jasmonates: A review. Journal of Horticultural Science and Biotechnology 83,283-304
112. Roitsch T, Ehness R (2000) Regulation of source/sink relations by cyto-kinins. Plant Growth Regulation 32,359-367
113. Rong L, Lei J, Wang C (2011) Collection and evaluation of the genus Lilium resources in Northeast China. Genetic Resources and Crop Evolution 58, 115-123
114. Ross HA, Davles HV, Burch LR, Viola R, McRae D (1994) Developmental changes in carbohydrate content and sucrose degrading enzyme in tuberizing stolon of potato (Solanum tuberosum L.). Physiologia Plantarum 90, 784-756
115. Sakakibara H (2006) Cytokinins: Activity, biosynthesis, and translocation. Annual Review of Plant Biology 57,431-449
116. Saniewski M, Horbowicz M (2004) Changes in endogenous flavonoids level during cold storage of tulip bulbs. Acta Horticulturae 669,245-252
117. Saniewski M, Kawa-Miszczak L, Wegrzynowicz-Leslak E, Okubo H (2000) Role of ABA, gibberellins and auxins in dormancy and dormancy release of tulip bulbs. In: Viemont J-D, Crabble J (Eds) Dormancy in Plants: From Whole Plant Behaviour to Cellular Control, CAB Intematio-nal, Oxon, UK, pp 227-243
118. Saniewsld M, Okubo H (2005) Only full cooling of mother tulip bulb is necessary for daughter bulb formation from daughter buds. Acta Horticulturae 673, 501-508
119. Saniewsld M, Puchalskl J (1987) The effect of methyl jasmonate and abscisic acid on differentiation of benzyladenine-induced bulblets in Muscari bulbs. Biologia Plantarum 29,63-65
120. Santos I, Salema R (2000) Promotion by jasmonic acid of bulb formation in shoot cultures of Narcissus triandrus L. Plant Growth Regulation 30, 133-138
121. Santos J, Santos I, Salema R (1998) In vitro production of bulbs of Narcissus bulbocodium flowering in the first season of growth. Scientia Horiculturae 76,205-217
122. Sarkar D (2008) The signal transduction pathways controlling in planta tuberization in potato: An emerging synthesis. Plant Cell Reports 21,1-8
123. Sarkar D, Pandey SK, Sharma S (2006) Cytokinins antagonize the jasmo-nates action on the regulation of potato (Solanum tuberosum) tuber formation in vitro. Plant Cell, Tissue and Organ Culture 87,285-295
124. Sengupta J, Sen S (1988) Karyological analysis of cultured cells and regenerated plants of Cipura paludosa Aubl. (Iridaceae)-a structural hybrid. Bulletin of the Torrey Botanical Club 115, 280-289
125. Skorid M, Živković S, Savic J, Siler B, Sabovljevic A, Todorovic S, GrubiSic D (2012) Efficient one-step tissue culture protocol for propagation of endemic plant, Lilium martagon var. cattaniae Vis. African Journal of Biotechnology 11,1862-1867
126. Staikidou I, Selby C, Hanks G (2006) Stimulation of in vitro bulblet growth in Galanthus species with sucrose and activated charcoal. Acta Horticulturae 725,421-426
127. Staikidou I, Watson S, Harvey B.MR, Selby C (2005) Narcissus bulblet formation in vitro: Effect of carbohydrate type and osmolality of the culture medium. Plant Cell, Tissue and Organ Culture 80,313-320
128. Steinitz B, Cohen A, Goldberg Z, Kochba M (1991) Precocious gladiolus corm formation in liquid shake cultures. Plant Cell, Tissue and Oigan Culture 26,63-70
129. Sultana J, Sultana N, Siddique MNA, Islam AKMA, Hossaln MM, Hossain T (2010) In vitro bulb production in hippeastrum (Hippeastrum hybridum). Journal Central European of Agriculture 11,469-474
130. Swart PA, Kulkarni MG, Bairu MW, Finnie JF, Van Staden J (2012) Micro-propagation of Romulea sabulosa Schltr. ex Beg.-A potential ornamental plant. Scientia Horticulturae 135, 151-156
131. Taeb AQ Alderson PG (1990a) Effect of low temperature and sucrose on bulb development and on the carbohydrate status of bulbing shoots of tulip in vitro. Journal of Horticultural Science 65, 193-197
132. Taeb AG, Alderson PG (1990b) Shoot production and bulbing of tulip in vitro related to ethylene. Journal of Horticultural Science 65, 199-204
133. Takayama S, Misawa M (1980) Differentiation in Lilium bulbscales grown in vitro. Effects of activated charcoal, physiological age of bulbs and sucrose concentration on differentiation and scale leaf formation in vitro. Physiologia Plantarum 48, 121-125
134. Taylor JLS, Van Staden J (2001) In vitro propagation of Eucomis UHerit species-plant with medicinal and horticultural potential. Plant Growth Regulation 34, 317-329
135. Thakur R, Sood A, Nagar PK, Pandey S, Sobti RC, Ahuja PS (2005) Regulation of growth of Lilium plantlets in liquid medium by application of paclo-butrazol or ancymidol, for its amenabilty in a bioreactor system: Growth parameters. Plant Cell Reports 25,382-391
136. Thomas TD (2007) High-frequency, direct bulblet induction from rhizome ex-plants Curculigo orchioides Gaertn., an endangered medicinal herb. In Vitro Cellular and Developmental Biology-Plant 43,442-448
137. Thompson MR, Douglas TJ, Obata-Sasamoto H, Thorpe TA (1986) Man-nitol metabolism in cultured plant cells. Physiologia Plantarum 67, 365-369
138. Uranbey S (2010) In vitro bulblet regeneration from immature embryos of Muscari azureum. African Journal of Biotechnology 9,5121-5125
139. Van Aartrijk J (1984) Adventitious bud formation from bulb-scale explants of Lilium speciosum Thunb. in vitro. PhD dissertation, University of Wagenin-gen, 79 pp
140. Van Aartrijk J, Blom-Barnhoorn GJ (1981) Growth regulator requirements for adventitious regeneration from Lilium bulb-scale tissue in vitro, in relation to duration of bulb storage and cultivar. Scientia Horticulturae 14, 193-197
141. Varshney A, Dhavan V, Srivastava PS (2000) A protocol for in vitro mass propagation of Asiatic hybrids of lily through liquid stationary culture. In Vitro Cellular and Developmental Biology-Plant 36,383-391
142. Viola R, Roberts AG, Haupt S, Gazzani S, Hancock RD, Marmlrili N, Machray GC, Oparka KJ (2001) Tuberizatio in potato involves a switch from apoplastic to symplastic phloem unloading. Plant Cell 13,385-398
143. Vishnevetsky J, Zamskl I, Ziv A (2003) Enhancement bud and bulblet regeneration from bulbs of Nerine sarniensis cultured in vitro. Plant Cell Reports 21, 645-650
144. Vreugdenhil D, Van Dijk W (1989) Effects of ethylene on the tuberization of the potato (Solanum tuberosum) cuttings. Plant Growth Regulation 8,31-39
145. Vreughdenhil D, Xu X, Jung CS, Van Lammeren AAM, Ewing EE (1999) Initial anatomical changes associated with tuber formation on single node potato (Solanum tuberosum L.) cuttings: A re-evaluation. Annals of Botany 84,675-680
146. Werner T, SchmOUing T (2009) Cytokinin action in plant development. Current Opinion in Plant Biology 12, 527-538
147. Weryszko-Chmielewska E, Kozak D (2002) Anatomical changes in Gloriosa rothschildiana OBrien stems induced by JA-Me and ABA. Acta Horticulturae 570,430-570
148. Woodward AW, Barrel B (2004) Auxin: Regulation, action, and interaction. Annals of Botany 95,707-735
149. Xu R-Y (2007) Effect of low temperature on changes in endogenous hormone level and development in Lilium and Tulipa. PhD thesis, Niigata University, Japan,111 pp
150. Xu R-Y, Niimi Y, Han D-S (2006) Changes in endogenous abscisic asid and soluble sugars levels during dormancy-release in bulbs of Lilium rubellum. Scientia Horticulturae 111, 68-72
151. Xu X, Van Kammeren AAM, Vermeer E, Vreugdenhil D (1998a) The role of gibberellin, absicic acid, and sucrose in the regulation of potato tuber formation in vitro. Plant Physiology 117, 575-584
152. Xu X, Vreugdenhil D, Van Lammeren AAM (1998b) Cell division and cell enlargement during potato tuber formation. Journal of Experimental Botany 49, 573-582
153. Yamazaki H, Nishijima T, Koshioka M, Miura H (2002) Gibberellins do not act against abscisic acid in the rregulation of bulb dormancy of Allium wakegi Araki. Plant Growth Regulation 36, 223-229
154. Zaffryar S, Zimerman B, Abu-Abied M, Belausov E, Lurya G, Vainstein A, Kamenetsky R, Sadot E (2007) Development-specific association of amylo-plasts with microtubules in scale cell of Narcissus tazetta. Protoplasma 230, 153-163
155. Zakaria M, Hossain MM, Khaleque Mian MA, Hossain T, Sultana N (2007) Effect of nitrogen and potassium in in vitro tuberization of potato. Plant Tissue Culture and Biotechnology 17,79-85
156. Žel J, Debeljak N, Ucman R, Ravnikar M (1997) the effect of jasmonic acid, sucrose and darkness on garlic (Allium sativum L. cv. Ptujski Jesenski) bulb formation in vitro. In Vitro Cellular and Developmental Biology-Plant 33, 231-235
157. Zheng RR, Wu Y, Xia YP (2012) Chlorocholine chloride and paclobutrazol treatments promote carbohydrate accumulation in bulbs of Lilium Oriental hybrids Sorbonne. Journal ofZhejiang University Science B 13,136-144
158. Ziv M (1989) Enhanced shoot and cormlet proliferation in liquid cultured gladiolus buds by growth retardants. Plant Cell. Tissue and Organ Culture 17, 101-110
159. Ziv M (1990) The effect of growth retardants on shoot proliferation and morphogenesis in liquid cultured Gladiolus plants. Acta Horticulturae 280, 207-213
160. Ziv M (2005) Simple bioreactor for mass propagation of plants. Plant Cell, Tissue and Organ Culture 81, 277-285
161. Ziv M, Kahany S, Lilien-Kipnis H (1994) Scaled-up proliferation and regeneration of Nerine in liquid cultures. Part I. The induction and maintenance of proliferating meristematic clusters by paclobutrazol in bioreactors. Plant Cell, Tissue and Organ Culture 39,109-115