In Vitro Storage Organ Formation of Ornamental Geophytes

Horticultural Reviews

Volumn 34, Issue , 2008, Pages 417-445

  Ascough, Glendon D ^a   van Staden, Johannes ^a   Erwin, John E ^b  

^a UNIVERSITY OF KWAZULU NATAL   (South Africa)

^b UNIVERSITY OF MINNESOTA   (United States)

Author keywords

Adventitious bulblet formation; Geophytic organ in vitro production; In vitro grown plantlets; Ornamental geophyte; Storage organ formation in vitro

Indexed keywords

EID: 54849437355     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9780470380147.ch7     Document Type: Chapter

References (134)

1. Aguettaz, P., A. Paffen, I. Delvallée, P. van der Linde, and G.-J. de Klerk. 1990. The development of dormancy in bulblets of Lilium speciosum generated in vitro. Plant Cell Tiss. Org. Cult. 22:167-172.
2. Angulo, M.E., R. Colque, F. Viladomat, J. Bastida, and C. Codina. 2003. In vitro production of bulblets of Cyrtanthus loddigesianus and Cyrtanthus speciosus. J. Hort. Sci. Biotech. 78:441-446.
3. Arthur, G.D., W.A. Stirk, and J. van Staden. 2004. Screening of aqueous extracts from gelling agents (Agar and Gelrite) for root-stimulating activity. S. Afr. J. Bot. 70:595-601.
4. Arthur, G.D., W.A. Stirk, and J. van Staden. 2006. Effects of autoclaving and charcoal on root-promoting substances present in water extracts made from gelling agents. Bior. Tech. 97:1942-1950.
5. Ascough, G.D., and C.W. Fennell. 2004. Regulation of plant growth and development in liquid culture. S. Afr. J. Bot. 70:181-190.
6. Ascough, G.D., N.P. Mtshali, D.I. Thompson, N.O. Anderson, J.E. Erwin, and J. van Staden. 2006. Watsonia, pp. 599-605. In J. A. Teixera da Silva (ed.), Floriculture, Ornamental and Plant Biotechnology, vol. 4. Global Science Books, UK.
7. Bach, A., and A. Swiderski. 2000. The effect of light quality on organogenesis of Hyacinthus orientalis L. in vitro. Acta Biol. Cracov. Series Bot. 42:115-120.
8. Bach, A., A. Cecot, and M. Gaweda. 1992a. Growth and mineral uptake of Hyacinthus orientalis L. under different temperature treatment. Acta Hort. 325:481-485.
9. Bach, A., B. Pawlowska, and K. Pulczyńska. 1992b. Utilization of soluble carbohydrates in shoot and bulb regeneration of Hyacinthus orientalis L. in vitro. Acta Hort. 325:487-491.
10. Bairu, M.W., C.W. Fennell, and J. van Staden. 2006. The effect of plant growth regulators on somaclonal variation in Cavendish banana (Musa AAA cv. 'Zelig'. Scientia Hort. 108:347-351.
11. Chanteloube, F., J.-C. Courduruox, M. Tort, and M. Le Nard. 1995. Micropropagation of Tulipa gesneriana L.: Regeneration of bulblets on growing floral stem segments cultured in vitro. Acta Bot. Gallica 142:301-307.
12. Chiari, A., and M.P. Bridgen. 2000. Rhizome splitting: A new micropropagation technique to increase in vitro propagule yield in Alstroemeria. Plant Cell Tiss. Org. Cult. 62:39-46.
13. Chow, Y.N., C. Selby, and B.M.R. Harvey. 1992. Stimulation by sucrose of Narcissus bulbil formation in vitro. J. Hort. Sci. 67:289-293.
14. Colque, R., F. Viladomat, J. Bastida, and C. Codina. 2002. Micropropagation of the rare Eucrosia stricklandii (Amaryllidaceae) by twin scaling and liquid shake culture. J. Hort. Sci. Biotech. 77:739-743.
15. Creelman, R.A., and J.E. Mullet. 1997. Biosynthesis and action of jasmonates in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 48:355-381.
16. Dantu, P.K., and S.S. Bhojwani. 1987. In vitro propagation and corm formation in Gladiolus. Gartenbauwissenschaft 52:90-93.
17. Debeljak, N., M. Regvar, K.W. Dixon, and K. Sivasithamparam. 2002. Induction of tuberisation in vitro with jasmonic acid and sucrose in an Australian terrestrial orchid, Pterostylis sanguinea. Plant Growth Regul. 36:253-260.
18. Debergh, P.C.A. 1994. The in vitro techniques: Their contribution to breeding and multiplication of ornamentals. Acta. Hort. 353:122-133.
19. De Bruyn, M.H., and D.I. Ferreira. 1992. In vitro corm production of Gladiolus dalenii and G. tristis. Plant Cell Tiss. Org. Cult. 31:123-128.
20. De Bruyn, M.H., D.I. Ferreira, M.M. Slabbert, and J. Pretorius. 1992. In vitro propagation of Amaryllis belladonna. Plant Cell Tiss. Org. Cult. 31:179-184.
21. De Hertogh, A.A., and M. Le Nard. 1993. Physiological and biochemical aspects of flower bulbs, pp. 53-69. In A. A. De Hertogh and M. Le Nard (eds.), The physiology of flower bulbs. Elsevier, Amsterdam.
22. de Klerk, G.-J., K.-S. Kim, M. van Schadewijk, and M. Gerrits. 1992. Growth of bulblets of Lilium speciosum in vitro and in soil. Acta Hort. 325:513-516.
23. Delvalée, I., A. Paffen, and G.-J. de Klerk. 1990. The development of dormancy in bulblets of Lilium speciosum generated in vitro. II. The effect of temperature. Physiol. Plant. 80:431-436.
24. El Grari, R., and R.A. Backhaus. 1987. In vitro propagation of red squill, Urginea maritima Baker. Plant Cell Tiss. Org. Cult. 10:65-71.
25. Fennell, C.W. 2002. Crinum moorei: Propagation and secondary metabolite production in vitro. PhD thesis, Univ. Natal Press, Pietermaritzburg, South Africa.
26. Fennell, C.W., and J. van Staden. 2004. Biotechnology of South African bulbs. S. Afr. J. Bot. 70:37-46.
27. Fennell, C.W., N.R. Crouch, and J. van Staden. 2001. Micropropagation of the river lily, Crinum variabile (Amaryllidaceae). S. Afr. J. Bot. 67:74-77.
28. Finnie, J.F., and J. van Staden. 1989. In vitro propagation of Sandersonia and Gloriosa. Plant Cell Tiss. Org. Cult. 19:151-158.
29. Goeden, K.R., and C.B. Tong. 2003. The effects of blue light on potato tuberisation. Abstr, 543. Poster presented for the annual meeting of the Am. Soc. Plant Biol.
30. Han, B.H., H.J. Yu, B.W. Yae, and K.Y Paek. 2004. In vitro micropropagation of Lilium longiflorum 'Georgia' by shoot formation as influenced by addition of liquid medium. Scientia Hort. 103:39-49.
31. Hannweg, K., M.P. Watt, and P. Berjak. 1996.Asimple method for the micropropagation of Bowiea volubilis from inflorescence explants. Bot. Bul. Acad. Sin. 37:213-218.
32. Hauser, B., and W. Horn. 1991. In vitro corm formation of Sparaxis hybrids. Acta Hort. 300:169-172.
33. Havey, M.J., C.R. Galmarini, A.F. Gökçe, and C. Henson. 2004. QTL affecting soluble carbohydrate concentrations in stored onion bulbs and their association with flavor and health-enhancing attributes. Genome 47:463-468.
34. Hazarika, B.N. 2006. Morpho-physiological disorders in in vitro culture of plants. Scientia Hort. 108:105-120.
35. Hou, Q., J.G. Carman, and W.A. Varga. 1997. Micropropagation of Sego Lily. Plant Cell Tiss. Org. Cult. 49:149-151.
36. Huang, C.L., K.C. Chang, and H. Okubo. 2005. In vitro morphogenesis from pedicels of Hippeastrum×hybridum. J. Fac. Agr. Kyushu Univ. 50:27-33.
37. Hussey, G. 1975. Totipotency in tissue explants and callus of some members of the Liliaceae, Iridaceae, and Amaryllidaceae. J. Exp. Bot. 26:253-262.
38. Hussey, G. 1976a. Plantlet regeneration from callus and parent tissue in Ornithogalum thyrsoides. J. Expt. Bot. 27:375-382.
39. Hussey, G. 1976b. Propagation of Dutch iris by tissue culture. Scientia Hort. 4:163-165.
40. Hussey, G. 1977. In vitro propagation of Gladiolus by precocious axillary shoot formation. Scientia Hort. 6:387-296.
41. Ilahi, I., M. Jabeen, and N. Firdous. 1987. Morphogenesis with saffron tissue culture. J. Plant Physiol. 128:227-232.
42. Ilan, A., M. Ziv, and A.H. Halevy. 1995. Propagation and corm development of Brodiaea in liquid cultures. Scientia Hort. 63:101-112.
43. Ilczuk, A., T. Winkelmann, S. Richartz, M. Witomska, and M. Serek. 2005. In vitro propagation of Hippeastrum×chmielii Chm.- influence of flurprimidol and the culture in solid or liquid medium and in temporary immersion systems. Plant Cell Tiss. Org. Cult. 83:339-346.
44. Jacobs, G., M. Richard, L.A. Allderman, and K.I. Theron. 1992. Direct and indirect organogenesis in tissue cultures of Nerine bowdenii Watts. Acta Hort. 325:475-479.
45. Jao, R.C., C.C. Lai, W. Fang, and S.F. Chang. 2005. Effects of red light of Zantedeschia plantlets in vitro and tuber formation using light-emitting diodes. HortScience 40: 436-438.
46. Jha, S., G.C. Mitra, and S. Sen. 1984. In vitro regeneration from bulb explants of Indian squill, Urginea indica Kunth. Plant Cell Tiss. Org. Cult. 3:91-100.
47. Jha, S., N.P. Sahu, and S.B. Mahato. 1991. Callus induction, organogenesis and somatic embryogenesis in three chromosomal races of Urginia indica and production of bufadienolides. Plant Cell Tiss. Org. Cult. 25:85-90.
48. Jordan, M., A. Amenábar, and C. Roveraro. 2002. Rapid in vitro propagation and microtuber production in Ullucus tuberosus (Basellaceae). Gartenbauwissenschaft 67: 50-54.
49. Kamenetsky, R., H. Zemah, A.P. Ranwala, F. Vergeldt, N.K. Ranwala, W.B. Miller, H. Van As, and P. Bendel. 2003. Water status and carbohydrate pools in tulip bulbs during dormancy release. New Phytol. 158:109-118.
50. Kamerbeek, G.A., J.C.M. Beijerbergen, and P.K. Schenk. 1970. Dormancy in bulbs and corms. Proc. 18th Int. Hort. Congr. 5:233-239.
51. Karam, N.S., and M. Al-Majathoub. 2000. Direct shoot regeneration and microtuberization in wild Cyclamen persicum Mill. using seedling tissue. Scientia Hort. 86:235-246.
52. Kim, K.-W., and A.A. De Hertogh. 1997. Tissue culture of ornamental flowering bulbs (geophytes). Hort. Rev. 18:87-169.
53. Kim, K.-S., and G.-W. Jang. 2004. Micropropagation of Drosera peltata, a tuberous sundew, by shoot tip culture. Plant Cell Tiss. Org. Cult. 77:211-214.
54. Kim, K.-S., E. Davelaar, and G.-J. de Klerk. 1994. Abscisic acid controls dormancy development and bulb formation in lily plantlets regenerated in vitro. Physiol. Plant. 90:59-64.
55. Kim, Y.-J., P.M. Hasegawa, and R.A. Bressan. 1981. In vitro propagation of hyacinth. HortScience 16:645-647.
56. Koda, Y. 1997. Possible involvement of jasmonates in various morphogenic events. Physiol. Plant. 100:639-646.
57. Kongbangkerd, A., A. Köpf, P. Allacher, C. Wawrosch, and B. Kopp. 2005. Micropropagation of squill (Charybdis numidica) through nodule culture. Plant Cell Rep. 23:673-677.
58. Krikorian, A.D., and R.P. Kann. 1986. Regeneration in Liliaceae, Iridaceae, and Amaryllidaceae, pp. 187-205. In I. K. Vasil (ed.), Cell culture and somatic cell genetics of plants. Vol. 3, Academic Press, Orlando, FL.
59. Kromer, K.D. 1985. Regeneration of some monocotyledonous plants from subterranean organs in vitro. Acta Agrobot. 38:65-87.
60. Kromer, K.D. 1989. The effect of light conditions on regeneration and level of endogenous growth regulators in Muscari racemosum (L.) Mill. Bulb scale sections cultured in vitro. Acta Hort. 251:173-181.
61. Kromer, K.D., and K. Kukulczanka. 1992. Control of morphogenesis in thin cell layer explants of Muscari botryoides Mill. Acta Hort. 325:505-512.
62. Kukulczanka, K., K.D. Kromer, and B. Czastka. 1989. Propagation of Fritillaria meleagris L. through tissue culture. Acta Hort. 251:147-153.
63. Legnani, G., and W.B. Miller. 2001. Short photoperiods induce fructan accumulation and tuberous root development in Dahlia seedlings. New Phytol. 149:449-454.
64. Le Nard, M., and A.A. De Hertogh. 1993. Bulb growth and development and flowering. In A.A. De Hertogh and M. Le Nard (eds.), The physiology of flower bulbs. Elsevier, Amsterdam.
65. Leshem, B., H. Lilien-Kipnis, and B. Steinitz. 1982. The effect of light and of explant orientation on the regeneration and subsequent growth of bulblets on Lilium longiflorum Thunb. bulb-scale sections cultured in vitro. Scientia Hort. 17:129-136.
66. Lian, M.L., D. Chakrabarty, and K.Y. Paek. 2003. Bulblet formation from bulbscale segments of Lilium using bioreactor system. Biol. Plant. 46:199-203.
67. Lilien-Kipnis, H., N. Azizbekova, and M. Ziv. 1994. Scaled-up proliferation and regeneration of Nerine in liquid cultures Part II. Ontogeny of somatic embryos and bulblet regeneration. Plant Cell Tiss. Org. Cult. 39:117-123.
68. Lilien-Kipnis, H., M. Ziv, S. Kahany, and N. Azizbekov. 1992. Proliferation and regeneration of Nerine on liquid culture. Acta Hort. 325:467-473.
69. Lim, S., J.H. Seon, K.Y. Paek, S.H. Son, and B.H. Han. 1998. Development of pilot scale process for mass production of Lilium bulblets in vitro. Acta Hort. 461: 237-241.
70. Madubanya, L.A. 2004. In vitro conservation of endangered Dierama species. M.Sc. thesis, Univ. KwaZulu-Natal Press, Pietermaritzburg, South Africa.
71. Maesato, K., K. Sharada, H. Fukui, T. Hara, and K.S. Sarma. 1994. In vitro bulblet regeneration from bulbscale explants of Lilium japonicumThunb. Effect of plant growth regulators and culture environment. J. Hort. Sci. 69:289-297.
72. Malabadi, R.B., and J. van Staden. 2004. Regeneration of Ornithogalum in vitro. S. Afr. J. Bot. 70:618-621.
73. Marinangeli, P., R. Devalis, S. Delmastro, and N. Curvetto. 1995. Stimulation of bulblet differentiation by traumatic acid in Lilium bulb-scale segments cultured in vitro. Biocell 19:189-195.
74. Marinangeli, P., S. Delmastro, and N. Curvetto. 1998. Influence of DB-cAMP, adenosine, forskolin, and traumatic acid on in vitro bulbing of Lilium longiflorum. HortScience 33:151-152.
75. Martínez-García, J. F., J. L. García-Martínez, J. Bou, and S. Prat. 2002. The interaction of gibberellins and photoperiod in the control of potato tuberization. J. Plant Growth Regul. 20:377-386.
76. McCartan, S.A. 1999. In vitro propagation of Scilla natalensis Planch. PhD Thesis, Univ. Natal Press, Pietermaritzburg, South Africa.
77. McCartan, S.A., R.P. Beckett, M.V. Ivanova, and J. van Staden. 2004. In vitro hardening- the role of acclimation stress in Kniphofia leucocephala. Plant Growth Regul. 43:49-55.
78. Milyaeva, E.L., N.S. Azizbekova, E.N. Komarova, and D.D. Akhundova. 1995. In vitro formation of regenerant corms of saffron crocus (Crocus sativus L.). Russ. J. Plant Physiol. 42:112-119.
79. Mirici, S., S. Parmaksiz, S. Ozcan, C. Sancak, S. Uranbey, E.O. Sarihan, A. Gumuscu, B. Gurbuz, and N. Arslan. 2005. Efficient in vitro bulblet regeneration from immature embryos of endangered Sternbergia fischeriana. Plant Cell Tiss. Org. Cult. 80:239-246.
80. Mochtak, E. 1989. Bulblet formation from the explants of Nerine bowdennii W. Wats. Acta Hort. 251:199-203.
81. Montezuma-de-Carvalho, J., and M.L.L. Guimaraes. 1974. Production of buds on plantlets from the stamen's filament of Lilium regale cultivated in vitro. Biol. Plant. 16:472-473.
82. Morán, G.P., R. Colque, F. Viladomat, J. Bastida, and C. Codina. 2003. Mass propagation of Cyrtanthus clavatus and Cyrtanthus spiralis using liquid medium culture. Scientia Hort. 98:49-60.
83. Mukhopadhyay, K., and S.C. Roy. 1994. In vitro induction of 'runner' -a quick method of micropropagation in orchid. Scientia Hort. 56:331-337.
84. Mukhopadhyay, M.J., S. Mukhopadhyay, and S. Sen. 2002. In vitro propagation of Iphigenia indica, an alternative source of colchicine. Plant Cell Tiss. Org. Cult. 69: 101-104.
85. Ngugi, G.W., A.K. Jäger, and J. Van Staden. 1998. In vitro propagation of Drimia robusta Bak. S. Afr. J. Bot. 64:266-268.
86. Nhut, D. T. 1998. Micropropagation of lily (Lilium longiflorum) via in vitro stem node and pseudo-bulblet culture. Plant Cell Rep. 17:913-916.
87. Nhut, D.T., J.A. Teixeira da Silva, P.X. Huyen, and K.Y. Peak. 2004. The importance of explant source on regeneration and micropropagation of Gladiolus by liquid shake culture. Scientia Hort. 102:407-414.
88. Niimi, Y., and T. Onozawa. 1979. In vitro bulblet formation from leaf segments of lilies, especially Lilium rubellum Baker. Scientia Hort. 11:379-389.
89. Oran, S.A., and I.A. Fattash. 2005. In vitro propagation of an endangered medicinal bulbous plant Sternbergia clusiana Ker-Gawler (Amaryllidaceae). J. Hort. Sci. Biotech. 80:399-402.
90. Paek, K.Y., and H.N. Murthy. 2002. High frequency of bulblet regeneration from bulb scale sections of Fritillaria thunbergii. Plant Cell Tiss. Org. Cult. 68:247-252.
91. Paek, K.Y., and T.A. Thorpe. 1983. Hyacinth, pp. 479-509. In P.V. Ammirato, D.R. Evans, W.R. Sharp, and Y.P.S. Bajaj (eds.), Handbook of plant cell culture, Vol. 5. McGraw-Hill, New York.
92. Pan, M.J., and J. van Staden. 1998. The use of charcoal in in vitro culture-a review. Plant Growth Regul. 26:155-163.
93. Patil, V.M. 1998. Micropropagation studies in Ceropegoa spp. In Vitro Cell. Dev. Biol. Plant 34:240-243.
94. Peck, D.E., and B.G. Cumming. 1986. Beneficial effects of activated charcoal on bulblet production in tissue cultures of Muscari armeniacum. Plant Cell Tiss. Org. Cult. 6:9-14.
95. Pierik, R.L.M. 1991. Micropropagation of ornamental plants. Acta Hort. 289:45-53.
96. Pierik, R.L.M., and M.A. Ruibing. 1973. Regeneration of bulblets on bulb scale segments of hyacinth in vitro. Neth. J. Agr. Sci. 21:129-138.
97. Pierik, R.L.M., and H.H.M. Steegmans. 1975. Effect of auxins, cytokinins, gibberellins, abscisic acid and ethephon on regeneration and growth of bulblets on excised bulb scale segments of hyacinth. Physiol. Plant. 34:14-17.
98. Piqueras, A.B., H. Han, J. Escribano, C. Rubio, E. Hellin, and J.A. Fernandez. 1999. Development of cormogenic nodules and microcorms by tissue culture, a new tool for the multiplication and genetic improvement of saffron. Agronomie 19:603-610.
99. Preece, J.E., and E.G. Sutter. 1991. Acclimatization of micropropagated plants to the greenhouse and field, pp. 71-93. In P.C. Debergh and R.H. Zimmerman (eds.), Micropropagation: Technology and application. Kluwer Academic Pub. Dordrecht, the Netherlands.
100. Rees, A.R. 1972. The growth of bulbs. Academic Press, London.
101. Rees, A.R. 1992. Ornamental bulbs, corms and tubers. CAB Int., Wallingford, UK.
102. Rice, R.D., P.G. Alderson, and N.A. Wright. 1983. Induction of bulbing of tulip shoots in vitro. Scientia Hort. 20:377-390.
103. Robb, S.A. 1957. The culture of excised tissue from bulb scales of Lilium speciosum Thun. J. Expt. Bot. 8:348-352.
104. Roy, J., and N. Banerjee. 2002. Rhizome and shoot development during in vitro propagation of Geodorum densiflorum (Lam.) Schltr. Scientia Hort. 94:181-192.
105. Ruffoni, B., L. Semeria, M. Laura, M. Savona, and A. Bisio. 2005. In vitromorphogenesis and micropropagation of Anemone coronaria L. hybrids. Prop. Ornamen. Plants 2:74-77.
106. Rybczynski, J.J., and H. Gomolinska. 1989. 6-benzyladenine control of the initial bulblets formation of wild liliy Lilium martagon L. Acta Hort. 251:183-189.
107. Saniewski, M., J. Nowak, and R. Rudnicki. 1974. Studies on the physiology of hyacinth bulbs (Hyacinthus orientalis L.). IV. Hormonal regulation of induction of roots and bulblets in Hyacinthus orientalis L. grown in culture. Plant Sci. Lett. 2:373-376.
108. Santos, I., and R. Salema. 2000. Promotion by jasmonic acid of bulb formation in shoot cultures of Narcissus triandrus L. Plant Growth Regul. 30:133-138.
109. Santos, A., F. Fidalgo, I. Santos, and R. Salema. 2002. In vitro bulb formation of Narcissus asturiensis, a threatened species of the Amaryllidaceae. J. Hort. Sci. Biotech. 77:149-152.
110. Santos, J., I. Santos, and R. Salema. 1998. In vitro production of bulbs of Narcissus bulbocodium flowering in the first season of growth. Scientia Hort. 76:205-217.
111. Scholten, H.J., and R.L.M. Pierek. 1998. Agar as a gelling agent: differential biological effects in vitro. Scientia Hort. 77:109-116.
112. Sen, J., and S. Sen. 1995. Two-step bud culture technique for a high frequency regeneration of Gladiolus corms. Scientia Hort. 64:133-138.
113. Seon, J.H., Y.S. Kim, S.H. Son, and K.Y. Paek. 2000. The fed-batch culture system using bioreactors for the bulblets production of oriental lilies. Acta Hort. 520:53-59.
114. Sevimay, C.S., K.M. Khawar, I. Parmaksiz, S. Cocu, C. Sancak, E.O. Sarihan, and S. Ozcan. 2005. Prolific in vitro bulblet formation from bulb scales of meadow lily (Lilium candidum L.). Period. Biol. 107:107-111.
115. Sharp, W.R., R.S. Raskin, and H.E. Sommer. 1971. Haploidy in Lilium. Phytomorphology 21:334-347.
116. Sheridan, W.F. 1968. Tissue culture of the monocot Lilium. Planta 82:189-192.
117. Shimasaki, K., and Y. Fukumoto. 2000. In vitro bulbing in Lilium×formolongi Hort seedlings. Acta Hort. 520:61-65.
118. Sivakumar, G., K.V. Krishnamurthy, and T.D. Rajendran. 2003. In vitro corm production in Gloriosa superba L., an Ayurvedic medicinal plant. J. Hort. Sci. Biotech. 78:450-453.
119. Slabbert, M.M., and J.G. Niederwieser. 1999. In vitro bulblet production of Lachenalia. Plant Cell Rep. 18:620-624.
120. Slabbert, M.M., M.H. de Bruyn, D.I. Ferreira, and J. Pretorius. 1993. Regeneration of bulblets from twin scales of Crinum macowanii in vitro. Plant Cell Tiss. Org. Cult. 33:133-141.
121. Staikidou, I., C. Selby, and G.R. Hanks. 2006. Development of a medium for in vitro culture of Galanthus species based on the mineral composition of the bulbs. J. Hort. Sci. Biotechnol. 81:537-545.
122. Staikidou, L., S. Watson, B.M.R. Harvey, and C. Selby. 2005. Narcissus bulblet formation in vitro: effects of carbohydrate type and osmolarity of the culture medium. Plant Cell Tiss. Org. Cult. 80:313-320.
123. Steinitz, B., and H. Yahel. 1982. In vitro propagation of Narcissus tazetta. HortScience 17:333-334.
124. Sutter, E.G. 1986. Micropropagation of Ixia viridifolia and a Gladiolus×Homoglossum hybrid. Scientia Hort. 29:181-189.
125. Takayama, S., and M. Misawa. 1982/1983. A scheme for mass propagation of Lilium in vitro. Scientia Hort. 18:353-362.
126. Ulrich, M.R., F.T. Davies Jr., Y.C. Koh, S.A. Duray, and J.N. Egilla. 1999. Micropropagation of Crinum 'Ellen Bosanquet' by tri-scales. Scientia Hort. 82:95-102.
127. van der Linde, P.C.G. 1992. Tissue culture of flower-bulb crops: Theory and practice. Acta Hort. 325:419-428.
128. Yanagawa, T., and Y. Sakanishi. 1980. Studies on the regeneration of excised bulb tissues of various tunicated-bulbous ornamentals. I: Regenerative capacity of the segments from different parts of bulb scales. J. Japan. Soc. Hort. Sci. 46:495-502.
129. Yi, Y.-B, K.-S. Lee, and C.-H. Chung. 2002. Protein variation and efficient in vitro culture of scale segments from Hyacinthus orientalis L. cv. Carnegie. Scientia Hort. 92:367-374.
130. Zhou, S.P., Y.K. He, and S.J. Li. 1999. Induction and characterisation of in vitro corms of diploid-taro. Plant Cell Tiss. Org. Cult. 57:173-178.
131. Ziv, M. 1989. Enhanced shoot and cormlet proliferation in liquid cultured Gladiolus buds by growth retardants. Plant Cell Tiss. Org. Cult. 17:101-110.
132. Ziv, M., and H. Lilien-Kipnis. 1983. Gladiolus, pp. 461-478. In P.V. Ammirato, D.R. Evans, W.R. Sharp, and Y.P.S. Bajaj (eds.), Handbook of plant cell culture, Vol. 5. McGraw-Hill, New York.
133. Ziv, M., and H. Lilien-Kipnis. 2000. Bud regeneration from inflorescence explants for rapid propagation of geophytes in vitro. Plant Cell Rep. 19:845-850.
134. Ziv, M., G. Ronen, and M. Raviv. 1998. Proliferation of meristematic clusters in disposable presterilised plastic bioreactors for the large-scale micropropagation of plants. In vitro Cell. Dev. Biol.- Plant. 34:152-158.