Enhancement of free fatty acid production in Saccharomyces cerevisiae by control of fatty acyl-CoA metabolism

Applied Microbiology and Biotechnology

Volumn 98, Issue 15, 2014, Pages 6739-6750

  Chen, Liwei ^a   Zhang, Jianhua ^a   Lee, Jaslyn ^a   Chen, Wei Ning ^a  

^a Nanyang Technological University   (Singapore)

Author keywords

Acyl CoA synthetase; Acyl CoA thioesterase; Free fatty acids; Saccharomyces cerevisiae

Indexed keywords

BIOFUELS; CYTOLOGY; ENCODING (SYMBOLS); GENES; METABOLISM; PHYSIOLOGY; UNSATURATED FATTY ACIDS;

ACYL-COA SYNTHETASE; FATTY ACID SYNTHESIS; FREE FATTY ACID; GROWTH INHIBITION; PETROLEUM-DERIVED FUELS; STATIONARY PHASE; THIOESTERASES; TRUNCATED VERSIONS;

YEAST;

ACYL COENZYME A; FATTY ACID; LONG CHAIN FATTY ACID COENZYME A LIGASE; PALMITOYL COENZYME A HYDROLASE; UNSATURATED FATTY ACID; FAA1 PROTEIN, S CEREVISIAE; FAA4 PROTEIN, S CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEIN; THIOL ESTER HYDROLASE;

ARTICLE; BACTERIAL GROWTH; BACTERIAL METABOLISM; BACTERIAL STRAIN; CONTROLLED STUDY; CYTOPLASM; ENZYME METABOLISM; GENE DELETION; HAPLOIDY; MOUSE; MUS MUSCULUS; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; PROTEIN FOLDING; SACCHAROMYCES CEREVISIAE; ANIMAL; BIOSYNTHESIS; ENZYMOLOGY; GENETICS; METABOLIC ENGINEERING; METABOLISM; UPREGULATION;

ACYL COENZYME A; ANIMALS; COENZYME A LIGASES; FATTY ACIDS, NONESTERIFIED; GENE DELETION; METABOLIC ENGINEERING; MICE; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; THIOLESTER HYDROLASES; UP-REGULATION;

EID: 84905060763     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-014-5758-8     Document Type: Article

References (46)

1. Black PN, DiRusso CC (2007) Yeast acyl-CoA synthetases at the crossroads of fatty acid metabolism and regulation. Biochim Biophys Acta Mol Cell Biol Lipids 1771(3):286-298. doi:10.1016/j.bbalip.2006.05.003 (Pubitemid 46437375)
2. Bortz WM, Lynen F (1963) The inhibition of acetyl CoA carboxylase by long chain acyl CoA derivatives. Biochem Z 337:505-509
3. Browse J, McCourt PJ, Somerville CR (1986) Fatty acid composition of leaf lipids determined after combined digestion and fatty acidmethyl ester formation from fresh tissue. Anal Biochem 152(1):141-145. doi:10.1016/0003-2697(86)90132- 6 (Pubitemid 16140446)
4. Chen L, Zhang J, Chen WN (2014) Engineering the Saccharomyces cerevisiae β-oxidation pathway to increase medium chain fatty acid production as potential biofuel. PLoS ONE 9(1):e84853. doi:10.1371/journal.pone.0084853
5. Cherry JM, Hong EL, Amundsen C, Balakrishnan R, Binkley G, Chan ET, Christie KR, Costanzo MC, Dwight SS, Engel SR, Fisk DG, Hirschman JE, Hitz BC, Karra K, Krieger CJ, Miyasato SR, Nash RS, Park J, Skrzypek MS, Simison M, Weng S, Wong ED (2012) Saccharomyces Genome Database: the genomics resource of budding yeast. Nucleic Acids Res 40(D1):D700-D705. doi:10.1093/nar/gkr1029
6. Choi YJ, Lee SY (2013) Microbial production of short-chain alkanes. Nature 502(7472):571-574. doi:10.1038/nature12536 http://www.nature.com/nature/ journal/v502/n7472/abs/nature12536.html#supplementary-information
7. Choi J-Y, Stukey J, Hwang S-Y, Martin CE (1996) Regulatory elements that control transcription activation and unsaturated fatty acid-mediated repression of the Saccharomyces cerevisiae OLE1 gene. J Biol Chem 271(7):3581-3589. doi:10.1074/jbc.271.7.3581 (Pubitemid 26065670)
8. Faergeman NJ, Knudsen J (1997) Role of long-chain fatty acyl-CoA esters in the regulation of metabolism and in cell signalling. Biochem J 323:1-12 (Pubitemid 27153867)
9. Færgeman NJ, Black PN, Zhao XD, Knudsen J, DiRusso CC (2001) The acyl-CoA synthetases encoded within FAA1 and FAA4 in Saccharomyces cerevisiae function as components of the fatty acid transport system linking import, activation, and intracellular utilization. J Biol Chem 276(40):37051-37059. doi:10.1074/jbc.M100884200
10. Feddersen S, Neergaard TBF, Knudsen J, Faergeman NJ (2007) Transcriptional regulation of phospholipid biosynthesis is linked to fatty acid metabolism by an acyl-CoA-binding-protein-dependent mechanism in Saccharomyces cerevisiae. Biochem J 407:219-230. doi:10.1042/bj20070315 (Pubitemid 47556987)
11. Gueldener U, Heinisch J, Koehler GJ, Voss D, Hegemann JH (2002) A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast. Nucleic Acids Res 30(6):e23
12. Hamilton J, Guo W, Kamp F (2002) Mechanism of cellular uptake of long-chain fatty acids: do we need cellular proteins? In: Glatz JC (ed) Cellular lipid binding proteins. Dev Mol Cell Biochem vol 38. Springer US, pp 17-23 (Pubitemid 35385791)
13. Horak T, Culik J, Cejka P, Jurkova M, Kellner V, Dvorak J, Haskova D (2009) Analysis of free fatty acids in beer: comparison of solid-phase extraction, solid-phase microextraction, and stir bar sorptive extraction. J Agric Food Chem 57(23):11081-11085. doi:10.1021/jf9028305
14. Howard TP, Middelhaufe S, Moore K, Edner C, Kolak DM, Taylor GN, Parker DA, Lee R, Smirnoff N, Aves SJ, Love J (2013) Synthesis of customized petroleum-replica fuel molecules by targeted modification of free fatty acid pools in Escherichia coli. Proc Natl Acad Sci. doi:10.1073/pnas.1215966110
15. Jiang P, Cronan JE (1994) Inhibition of fatty acid synthesis in Escherichia coli in the absence of phospholipid synthesis and release of inhibition by thioesterase action. J Bacteriol 176(10):2814-2821 (Pubitemid 24152924)
16. Jones JM, Nau K, Geraghty MT, Erdmann R, Gould SJ (1999) Identification of peroxisomal acyl-CoA thioesterases in yeast and humans. J Biol Chem 274(14):9216-9223. doi:10.1074/jbc.274.14.9216
17. Kamiryo T, Parthasarathy S, Numa S (1976) Evidence that acyl coenzyme A synthetase activity is required for repression of yeast acetyl coenzyme A carboxylase by exogenous fatty acids. Proc Natl Acad Sci U S A 73(2):386-390
18. Kamisaka Y, Kimura K, Uemura H, Yamaoka M (2013) Overexpression of the active diacylglycerol acyltransferase variant transforms Saccharomyces cerevisiae into an oleaginous yeast. Appl Microbiol Biotechnol 97(16):7345-7355. doi:10.1007/s00253-013-4915-9
19. Kamp F, Hamilton JA (2006) How fatty acids of different chain length enter and leave cells by free diffusion. Prostaglandins Leukot Essent Fat Acids 75(3):149-159. doi:10.1016/j.plefa.2006.05.003 (Pubitemid 44292518)
20. Knoll LJ, Johnson DR, Gordon JI (1994) Biochemical studies of three Saccharomyces cerevisiae acyl-CoA synthetases, Faa1p, Faa2p, and Faa3p. J Biol Chem 269(23):16348-16356 (Pubitemid 24209333)
21. Knoll LJ, Johnson DR, Gordon JI (1995) Complementation of Saccharomyces cerevisiae strains containing fatty acid activation gene (FAA) deletions with a mammalian acyl-CoA synthetase. J Biol Chem 270(18):10861-10867. doi:10.1074/jbc.270.18.10861
22. Krivoruchko A, Siewers V, Nielsen J (2011) Opportunities for yeast metabolic engineering: lessons from synthetic biology. Biotechnol J 6(3):262-276. doi:10.1002/biot.201000308
23. Leber C, Da Silva NA (2013) Engineering of Saccharomyces cerevisiae for the synthesis of short chain fatty acids. Biotechnol Bioeng. doi:10.1002/bit.25021
24. Leibundgut M, Maier T, Jenni S, Ban N (2008) The multienzyme architecture of eukaryotic fatty acid synthases. Curr Opin Struct Biol 18(6):714-725. doi:10.1016/j.sbi.2008.09.008
25. Li H, Melton EM, Quackenbush S, DiRusso CC, Black PN (2007) Mechanistic studies of the long chain acyl-CoA synthetase Faa1p from Saccharomyces cerevisiae. Biochim Biophys Acta Mol Cell Biol Lipids 1771(9):1246-1253. doi:10.1016/j.bbalip.2007.05.009 (Pubitemid 47404968)
26. Liu J-F, Nie K-L, Fan L-H, Wang F, Tan T-W, Deng L (2013) Increased production of FAEEs for biodiesel with lipase enhanced Saccharomyces cerevisiae. Process Biochem 48(8):1212 -1215. doi:10.1016/j.procbio.2013.06.003
27. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25(4):402-408. doi:10.1006/meth.2001.1262 (Pubitemid 34164012)
28. Lu XF, Vora H, Khosla C (2008) Overproduction of free fatty acids in E. coli: implications for biodiesel production. Metab Eng 10(6):333-339. doi:10.1016/j.ymben.2008.08.006
29. Maeda I, Delessert S, Hasegawa S, Seto Y, Zuber S, Poirier Y (2006) The peroxisomal acyl-CoA thioesterase Pte1p from Saccharomyces cerevisiae is required for efficient degradation of short straight chain and branched chain fatty acids. J Biol Chem 281(17):11729-11735. doi:10.1074/jbc.M511762200 (Pubitemid 43855431)
30. Magnuson K, Jackowski S, Rock CO, Cronan JE (1993) Regulation of fatty acid biosynthesis in Escherichia coli. Microbiol Rev 57(3):522-542 (Pubitemid 23262508)
31. Martin CE, Oh C-S, Jiang Y (2007) Regulation of long chain unsaturated fatty acid synthesis in yeast. Biochim Biophys Acta Mol Cell Biol Lipids 1771(3):271-285 (Pubitemid 46428610)
32. Oshiro T, Aiba H, Mizuno T (2003) A defect in a fatty acyl-CoA synthetase gene, lcf1+, results in a decrease in viability after entry into the stationary phase in fission yeast.Mol Gen Genomics 269(4):437-442. doi:10.1007/s00438-003- 0841-3 (Pubitemid 36976151)
33. Ramos MJ, Fernández CM, Casas A, Rodríguez L, Pérez Á (2009) Influence of fatty acid composition of raw materials on biodiesel properties. Bioresour Technol 100(1):261 -268. doi:10.1016/j.biortech. 2008.06.039
34. Ramsay RR, Gandour RD, van der Leij FR (2001) Molecular enzymology of carnitine transfer and transport. Biochim Biophys Acta Protein Struct Mol Enzymol 1546(1):21-43 (Pubitemid 32217800)
35. Russell Johnson D, Knoll LJ, Levin DE, Gordon JI (1994) Saccharomyces cerevisiae contains four fatty acid activation (FAA) genes: an assessment of their role in regulating protein N-myristoylation and cellular lipid metabolism. J Cell Biol 127(3):751-762 (Pubitemid 24332970)
36. Scharnewski M, Pongdontri P, Mora G, Hoppert M, Fulda M (2008) Mutants of Saccharomyces cerevisiae deficient in acyl-CoA synthetases secrete fatty acids due to interrupted fatty acid recycling. FEBS J 275(11):2765-2778. doi:10.1111/j.1742-4658.2008.06417.x (Pubitemid 351678664)
37. Schirmer A, Rude MA, Li XZ, Popova E, del Cardayre SB (2010) Microbial biosynthesis of alkanes. Science 329(5991):559-562. doi:10.1126/science.1187936
38. Sorger D, Daum G (2002) Synthesis of triacylglycerols by the acylcoenzyme A: diacyl-glycerol acyltransferase Dga1p in lipid particles of the yeast Saccharomyces cerevisiae. J Bacteriol 184(2):519-524. doi:10.1128/jb.184.2.519- 524.2002 (Pubitemid 34027393)
39. Steen EJ, Kang YS, Bokinsky G, Hu ZH, Schirmer A, McClure A, del Cardayre SB, Keasling JD (2010) Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature 463(7280):559-562. doi:10.1038/nature08721
40. Sumper M, Träuble H (1973) Membranes as acceptors for palmitoyl CoA in fatty acid biosynthesis. FEBS Lett 30(1):29-34. doi:10.1016/0014-5793(73) 80612-X
41. Tang X, Feng H, Chen WN (2013) Metabolic engineering for enhanced fatty acids synthesis in Saccharomyces cerevisiae. Metab Eng 16:95-102. doi:10.1016/j.ymben.2013.01.003
42. Torella JP, Ford TJ, Kim SN, Chen AM, Way JC, Silver PA (2013) Tailored fatty acid synthesis via dynamic control of fatty acid elongation. Proc Natl Acad Sci 110(28):11290-11295. doi:10.1073/pnas.1307129110
43. Voelker TA, Davies HM (1994) Alteration of the specificity and regulation of fatty acid synthesis of Escherichia coli by expression of a plant medium-chain acyl-acyl carrier protein thioesterase. J Bacteriol 176(23):7320-7327 (Pubitemid 24364059)
44. Wang MX, Bai J, Chen WN, Ching CB (2010) Metabolomic profiling of cellular responses to carvedilol enantiomers in vascular smooth muscle cells. PLoS ONE 5(11):e15441. doi:10.1371/journal.pone.0015441
45. Westin MAK, Alexson SEH, Hunt MC (2004) Molecular cloning and characterization of two mouse peroxisome proliferator-activated receptor α (PPARα)-regulated peroxisomal acyl-CoA thioesterases. J Biol Chem 279(21):21841-21848. doi:10.1074/jbc.M313863200 (Pubitemid 38684138)
46. Zheng Y-N, Li L-L, Liu Q, Yang J-M, Wang X-W, Liu W, Xu X, Liu H, Zhao G, Xian M (2012) Optimization of fatty alcohol biosynthesis pathway for selectively enhanced production of C12/14 and C16/18 fatty alcohols in engineered Escherichia coli. Microb Cell Factories 11(1):65