Comparison of culture based methods for the isolation of Clostridium difficile from stool samples in a research setting

Anaerobe

Volumn 28, Issue , 2014, Pages 226-229

  Lister, Michelle ^a   Stevenson, Emma ^a   Heeg, Daniela ^a   Minton, Nigel P ^a   Kuehne, Sarah A ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Clostridium difficile; Clostridium difficile infection; Culture media; Culture methods; Stool culture

Indexed keywords

CEFOXITIN; CYCLOSERINE; FRUCTOSE; LYSOZYME; MANNITOL; MISCELLANEOUS DRUGS AND AGENTS; NEUTRAL RED; TAUROCHOLIC ACID; TRYPTONE SOY AGAR; UNCLASSIFIED DRUG; CULTURE MEDIUM;

AGAR MEDIUM; ARTICLE; BACTERIUM CULTURE; BACTERIUM ISOLATION; BLOOD; CLOSTRIDIUM DIFFICILE; COST EFFECTIVENESS ANALYSIS; EGG YOLK; ENRICHMENT CULTURE; FECES ANALYSIS; FECES MICROFLORA; HEAT SHOCK; INTERMETHOD COMPARISON; NONHUMAN; PH; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; ANIMAL; CHEMISTRY; COMPARATIVE STUDY; COST BENEFIT ANALYSIS; CULTURE MEDIUM; ECONOMICS; EVALUATION STUDY; FECES; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; MESOCRICETUS; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; PEPTOCLOSTRIDIUM DIFFICILE; PROCEDURES; SENSITIVITY AND SPECIFICITY; TIME;

ANIMALS; BACTERIOLOGICAL TECHNIQUES; CLOSTRIDIUM DIFFICILE; COST-BENEFIT ANALYSIS; CULTURE MEDIA; FECES; MESOCRICETUS; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

EID: 84904888859     PISSN: 10759964     EISSN: 10958274     Source Type: Journal    
DOI: 10.1016/j.anaerobe.2014.07.003     Document Type: Article

References (15)

1. Jones A.M., Kuijper E.J., Wilcox M.H. Clostridium difficile: a European perspective. JInfect 2013, 66:115-128.
2. Carson K.C., Boseiwaqa L.V., Thean S.K., Foster N.F., Riley T.V. Isolation of Clostridium difficile from faecal specimens-a comparison of ChromID C. difficile agar and cycloserine cefoxitin fructose agar. JMed Microbiol 2013, 62:1423-1727.
3. Tyrrell K.L., Citron D.M., Leoncio E.S., Merriam C.V., Goldstein E.J. Evaluation of cycloserine-cefoxitin fructose agar (CCFA), CCFA with horse blood and taurocholate, and cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme for recovery of Clostridium difficile isolates from faecal samples. JClin Microbiol 2013, 51:3094-3096.
4. Perry J.D., Asir K., Halimi D., Orenga S., Dale J., Payne M., et al. Evaluation of a chromogenic culture medium for isolation of Clostridium difficile within 24 hours. JClin Microbiol 2010, 48:3852-3858.
5. Eckert C., Burghoffer B., Lalande V., Barbut F. Evaluation of the chromogenic agar ChromID C.difficile. JClin Microbiol 2013, 51:1002-1004.
6. Hill K., Collins J., Wilson L., Perry J., Gould F. Comparison of two selective media for the recovery of Clostridium difficile from environmental surfaces. JHosp Infect 2012, 83:164-166.
7. Clabots C.R., Bettin K.M., Peterson L.R., Gerding D. Evaluation of cycloserine-cefoxitin-fructose agar and cycloserine-cefoxitin-fructose broth for recovery of Clostridium difficile from environmental sites. JClin Microbiol 1991, 29:2633-2635.
8. Anaerobe Systems Cycloserine cefoxitin mannitol broth with taurocholate and lysozyme (CCMB-TAL) 2013, Available from:. http://www.anaerobesystems.com/Home/pras-tubed-media/cycloserine-cefoxitin-mannitol-broth-with-taurocholate-a%20.
9. Hink T., Burnham C.-A.D., Dubberke E.R. Asystematic evaluation of methods to optimize culture-based recovery of Clostridium difficile from stool specimens. Anaerobe 2013, 19:39-43.
10. Brazier J.S. Role of the laboratory in investigations of Clostridium difficile diarrhoea. Clin Infect Dis 1993, 16:S228-S233.
11. Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N., Stabler R., et al. The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome. Nat Genet 2006, 38:779-786.
12. Stabler R.A., He M., Dawson L., Martin M., Valiente E., Corton C., et al. Comparative genome and phenotypic analysis of Clostridium difficile 027 strains provides insight into the evolution of a hypervirulent bacterium. Genome Biol 2009, 10:R102.
13. Hussain H.A., Roberts A.P., Mullany P. Generation of an erythromycin-sensitive derivative of Clostridium difficile strain 630 (630δerm) and demonstration that the conjugative transposon Tn916δE enters the genome of this strain at multiple sites. JMed Microbiol 2005, 54:137-141.
14. Wilcox M., Fawley W., Parnell P. Value of lysozyme agar incorporation and alkaline thioglycollate exposure for the environmental recovery of Clostridium difficile. JHosp Infect 2000, 44:65-69.
15. Arroyo L.G., Rousseau J., Willey B.M., Low D.E., Staempfli H., McGeer A., et al. Use of a selective enrichment broth to recover Clostridium difficile from stool swabs stored under different conditions. JClin Microbiol 2005, 43:5341-5343.