Evaluation of cycloserine-cefoxitin fructose agar (CCFA), CCFA with horse blood and taurocholate, and cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme for recovery of Clostridium difficile isolates from fecal samples

Journal of Clinical Microbiology

Volumn 51, Issue 9, 2013, Pages 3094-3096

  Tyrrell, Kerin L ^a   Citron, Diane M ^a   Leoncio, Eliza S ^a   Merriam, C Vreni ^a   Goldstein, Ellie J C ^a,b  

^a R M ALDEN RESEARCH LABORATORY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AGAR; ALCOHOL; BACTERIAL TOXIN; CEFOXITIN; CYCLOSERINE; CYCLOSERINE CEFOXITIN FRUCTOSE AGAR; CYCLOSERINE CEFOXITIN FRUCTOSE AGAR WITH HORSE BLOOD AND TAUROCHOLATE; CYCLOSERINE CEFOXITIN MANNITOL BROTH WITH TAUROCHOLATE AND LYSOZYME; FRUCTOSE; INDICATOR; LYSOZYME; MANNITOL; TAUROCHOLIC ACID; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GROWTH; BACTERIUM COLONY; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CLINICAL ARTICLE; CLOSTRIDIUM DIFFICILE; CONTROLLED STUDY; ENRICHMENT CULTURE; ENZYME IMMUNOASSAY; FECES ANALYSIS; HUMAN; INTERMETHOD COMPARISON; NONHUMAN; PRIORITY JOURNAL; TOXIN ANALYSIS;

AGAR; ANIMALS; ANTI-INFECTIVE AGENTS; CEFOXITIN; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; CULTURE MEDIA; CYCLOSERINE; ERYTHROCYTES; FECES; FRUCTOSE; HORSES; HUMANS; MANNITOL; MURAMIDASE; TAUROCHOLIC ACID;

EID: 84882746262     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00879-13     Document Type: Article

References (11)

1. George WL, Sutter VL, Citron D, Finegold SM. 1979. Selective and differential medium for isolation of Clostridium difficile. J. Clin. Microbiol. 9:214-219.
2. Levett PN. 1985. Effect of antibiotic concentration in a selective medium on the isolation of Clostridium difficile from faecal specimens. J. Clin. Pathol. 38:233-234.
3. Wilson KH, Kennedy MJ, Fekety FR. 1982. Use of sodium taurocholate to enhance spore recovery on a medium selective for Clostridium difficile. J. Clin. Microbiol. 15:443-446.
4. Buggy BP, Hawkins CC, Fekety R. 1985. Effect of adding sodium taurocholate to selective media on the recovery of Clostridium difficile from environmental surfaces. J. Clin. Microbiol. 21:636-637.
5. Wilcox MH, Fawley WN, Parnell P. 2000. Value of lysozyme agar incorporation and alkaline thioglycollate exposure for the environmental recovery of Clostridium difficile. J. Hosp. Infect. 44:65-69.
6. Delmee M, Van BJ, Simon A, Janssens M, Avesani V. 2005. Laboratory diagnosis of Clostridium difficile-associated diarrhoea: a plea for culture. J. Med. Microbiol. 54(Part 2):187-191.
7. Tenover FC, Akerlund T, Gerding DN, Goering RV, Bostrom T, Jonsson AM, Wong E, Wortman AT, Persing DH. 2011. Comparison of strain typing results for Clostridium difficile isolates from North America. J. Clin. Microbiol. 49:1831-1837.
8. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth-KTL anaerobic bacteriology manual. Star Publishing, Belmont, CA.
9. Versalovic J, Carrol KC, Funke G, Jorgensen JH, Landry ML, Warnock DW. 2011. Manual of clinical microbiology. ASM Press, Washington, DC.
10. Tyrrell KL, Warren YA, Citron DM, Goldstein EJ. 2011. Re-assessment of phenotypic identifications of Bacteroides putredinis to Alistipes species using molecular methods. Anaerobe 17:130-134.
11. Marler LM, Siders JA, Wolters LC, Pettigrew Y, Skitt BL, Allen SD. 1992. Comparison of five cultural procedures for isolation of Clostridium difficile from stools. J. Clin. Microbiol. 30:514-516.