A systematic evaluation of methods to optimize culture-based recovery of Clostridium difficile from stool specimens

Anaerobe

Volumn 19, Issue 1, 2013, Pages 39-43

  Hink, Tiffany ^a   Burnham, Carey Ann D ^a   Dubberke, Erik R ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Alcohol shock; Asymptomatic carrier; Broth enrichment; C. difficile; C. difficile culture

Indexed keywords

CEFOXITIN; CYCLOSERINE; TAUROCHOLIC ACID;

AGAR DIFFUSION; ARTICLE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BROTH DILUTION; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM DIFFICILE INFECTION; COLONY FORMING UNIT; CONTROLLED STUDY; FECES ANALYSIS; HUMAN; PREVALENCE; PRIORITY JOURNAL; PROCESS OPTIMIZATION;

BACTERIOLOGICAL TECHNIQUES; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; CULTURE MEDIA; FECES; HUMANS; SENSITIVITY AND SPECIFICITY;

CLOSTRIDIUM DIFFICILE; OVIS ARIES;

EID: 84872822741     PISSN: 10759964     EISSN: 10958274     Source Type: Journal    
DOI: 10.1016/j.anaerobe.2012.12.001     Document Type: Article

References (15)

1. Cohen S.H., Gerding D.N., Johnson S., Kelly C.P., Loo V.G., McDonald L.C., et al. Clinical practice guidelines for Clostridium difficile infection in adults: 2010 update by the society for healthcare epidemiology of America (SHEA) and the infectious diseases society of America (IDSA). Infect Control Hosp Epidemiol 2010, 31(5):431-455.
2. Dubberke E.R. The A, B, BI, and Cs of Clostridium difficile. Clin Infect Dis 2009, 49(8):1148-1152.
3. Dubberke E.R. Prevention of healthcare-associated Clostridium difficile infection: what works?. Infect Control Hosp Epidemiol 2010, 1(31 Suppl):S38-S41.
4. Arroyo L.G., Rousseau J., Willey B.M., Low D.E., Staempfli H., McGeer A., et al. Use of a selective enrichment broth to recover Clostridium difficile from stool swabs stored under different conditions. J Clin Microbiol 2005, 43(10):5341-5343.
5. Bliss D.Z., Johnson S., Clabots C.R., Savik K., Gerding D.N. Comparison of cycloserine-cefoxitin-fructose agar (CCFA) and taurocholate-CCFA for recovery of Clostridium difficile during surveillance of hospitalized patients. Diagn Microbiol Infect Dis 1997, 29(1):1-4.
6. Clabots C.R., Gerding S.J., Olson M.M., Peterson L.R., Gerding D.N. Detection of asymptomatic Clostridium difficile carriage by an alcohol shock procedure. J Clin Microbiol 1989, 27(10):2386-2387.
7. Marler L.M., Siders J.A., Wolters L.C., Pettigrew Y., Skitt B.L., Allen S.D. Comparison of five cultural procedures for isolation of Clostridium difficile from stools. J Clin Microbiol 1992, 30(2):514-516.
8. Rodriguez-Palacios A., Staempfli H.R., Duffield T., Weese J.S. Clostridium difficile in retail ground meat, Canada. Emerg Infect Dis 2007, 13(3):485-487.
9. Songer J.G., Trinh H.T., Killgore G.E., Thompson A.D., McDonald L.C., Limbago B.M. Clostridium difficile in retail meat products, USA, 2007. Emerg Infect Dis 2009, 15(5):819-821.
10. Weese J.S., Avery B.P., Rousseau J., Reid-Smith R.J. Detection and enumeration of Clostridium difficile spores in retail beef and pork. Appl Environ Microbiol 2009, 75(15):5009-5011.
11. Wilcox M.H., Fawley W.N., Parnell P. Value of lysozyme agar incorporation and alkaline thioglycollate exposure for the environmental recovery of Clostridium difficile. J Hosp Infect 2000, 44(1):65-69.
12. Clabots C.R., Johnson S., Olson M.M., Peterson L.R., Gerding D.N. Acquisition of Clostridium difficile by hospitalized patients: evidence for colonized new admissions as a source of infection. J Infect Dis 1992, 166(3):561-567.
13. Lanzas C., Dubberke E.R., Lu Z., Reske K.A., Grohn Y.T. Epidemiological model for Clostridium difficile transmission in healthcare settings. Infect Control Hosp Epidemiol 2011, 32(6):553-561.
14. Riggs M.M., Sethi A.K., Zabarsky T.F., Eckstein E.C., Jump R.L., Donskey C.J. Asymptomatic carriers are a potential source for transmission of epidemic and nonepidemic Clostridium difficile strains among long-term care facility residents. Clin Infect Dis 2007, 45(8):992-998.
15. Walker A.S., Eyre D.W., Wyllie D.H., Dingle K.E., Harding R.M., O'Connor L., et al. Characterisation of Clostridium difficile hospital ward-based transmission using extensive epidemiological data and molecular typing. PLoS Med 2012, 9(2):e1001172.