Direct molecular detection of pathogens in blood as specific rule-In diagnostic biomarker in patients with presumed sepsis: Our experience on a heterogeneous cohort of patients with signs of infective systemic inflammatory response syndrome

Shock

Volumn 42, Issue 2, 2014, Pages 86-92

  Avolio, Manuela ^a   Diamante, Paola ^a   Modolo, Maria Luisa ^a   De Rosa, Rita ^a   Stano, Paola ^a   Camporese, Alessandro ^a  

^a Hospital S Maria degli Angeli   (Italy)

Author keywords

molecular diagnosis of sepsis; sepsis; SeptiFast

Indexed keywords

ANTIBIOTIC AGENT; BACTERIAL DNA; FUNGAL DNA;

ADULT; ANTIBIOTIC THERAPY; ARTICLE; BLOOD CULTURE; BLOODSTREAM INFECTION; CLINICAL TRIAL; CRITICALLY ILL PATIENT; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; DISEASE MARKER; FUNGUS; GRAM POSITIVE BACTERIUM; HUMAN; MAJOR CLINICAL STUDY; REAL TIME POLYMERASE CHAIN REACTION; SEPSIS; SYSTEMIC INFLAMMATORY RESPONSE SYNDROME; AGED; BACTEREMIA; BLOOD; BLOOD SAMPLING; COMPLICATION; EVALUATION STUDY; FUNGEMIA; KAPLAN MEIER METHOD; MICROBIOLOGY; MIDDLE AGED; PREDICTIVE VALUE; PROCEDURES; PROSPECTIVE STUDY; SENSITIVITY AND SPECIFICITY; YOUNG ADULT;

ADULT; AGED; BACTEREMIA; BLOOD SPECIMEN COLLECTION; DNA, BACTERIAL; DNA, FUNGAL; FUNGEMIA; HUMANS; KAPLAN-MEIER ESTIMATE; MIDDLE AGED; PREDICTIVE VALUE OF TESTS; PROSPECTIVE STUDIES; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEPSIS; SYSTEMIC INFLAMMATORY RESPONSE SYNDROME; YOUNG ADULT;

EID: 84904674023     PISSN: 10732322     EISSN: 15400514     Source Type: Journal    
DOI: 10.1097/SHK.0000000000000191     Document Type: Article

References (31)

1. Bone RC: Toward an epidemiology and natural history of SIRS (systemic inflammatory response syndrome). JAMA 268(24):3452-3455, 1992.
2. Fitting C, Parlato M, Adib-Conquy M, Memain N, Philippart F, Misset B, Monchi M, Cavaillon JM, Adrie C: DNAemia detection by multiplex PCR and biomarkers for infection in systemic inflammatory response syndrome patients. PLos One 7(6):1-7, 2012.
3. Levy MM, Fink MP, Marshall JC, Abraham E, Angus D, Cook D, Cohen J, Opal SM, Vincent JL, Ramsay G: 2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference. Crit Care Med 31(4):1250-1256, 2003.
4. Mauro MV, Cavalcanti P, Perugini D, Noto A, Sperli D, Giraldi C: Diagnostic utility of LightCycler SeptiFast and procalcitonin assays in the diagnosis of bloodstream infection in immunocompromised patients. Diagn Microbiol Infect Dis 73(4):308-311, 2012.
5. Avolio M, Diamante P, Zamparo S, Modolo ML, Grosso S, Zigante P, Tosoni N, De Rosa R, Stano P, Camporese A: Molecular identification of bloodstream pathogens in patients presenting to the emergency room with suspected sepsis. Shock 34(1):27-30, 2010.
6. Louie RF, Tang Z, Albertson TE, Cohen S, Tran NK, Kost GJ: Multiplex polymerase chain reaction detection enhancement of bacteremia and fungemia. Crit Care Med 36(5):1487-1492, 2008.
7. Lehmann LE, Hunfeld KP, Emrich T, Haberhausen G, Wissing H, Hoeft A, Stüber F: A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med Microbiol Immunol 197(3):313-324, 2008.
8. Pasqualini L, Mencacci A, Leli C, Montagna P, Cardaccia A, Cenci E, Montecarlo I, Pirro M, di Filippo F, Cistaro E, et al.: Diagnostic performance of a multiple real-time PCR assay in patients with suspected sepsis hospitalized in an internal medicine ward. J Clin Microbiol 50(4):1285-1288, 2012.
9. Leyssene D, Gardes S, Vilquin P, Flandrois JP, Carret G, Lamy B: Speciesdriven interpretation guidelines in case of a single-sampling strategy for blood culture. Eur J Clin Microbiol Infect Dis (30):1537-1541, 2011.
10. Lamoth F, Jaton K, Prodhom G, Senn L, Bille J, Calandra T, Marchetti O: Multiplex blood PCR in combination with blood cultures for improvement of microbiological documentation of infection in febrile neutropenia. J Clin Microbiol 48(10):3510-3516, 2010.
11. Peters RP, van Agtmael MA, Danner SA, Savelkoul PH, Vandenbroucke-Grauls CM: New developments in the diagnosis of bloodstream infections. Lancet Infect Dis 4(12):751-760, 2004.
12. Chaiyakunapruk N, Veenstra DL, Lipsky BA, Saint S: Chlorhexidine compared with povidone-iodine solution for vascular catheter-site care: a meta-analysis. Ann Intern Med 136(11):792-801, 2002.
13. Lee A, Mirrett S, Reller LB, Weinstein MP: Detection of bloodstream infections in adults: how many blood cultures are needed? J Clin Microbiol 45(11):3546-3548, 2007.
14. Weinstein MP: Blood culture contamination: persisting problems and partial progress. J Clin Microbiol 41(6):2275-2278, 2003.
15. Westh H, Lisby G, Breysse F, Böddinghaus B, Chomarat M, Gant V, Goglio A, Raglio A, Schuster H, Stuber F, et al.: Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis. Clin Microbiol Infect 15(6):544-551, 2009.
16. Dark PM, Dean P, Warhurst G: Bench-to-bedside review: the promise of rapid infection diagnosis during sepsis using polymerase chain reactionYbased pathogen detection. Crit Care 13:217, 2009.
17. Pletz MW, Wellinghausen N, Welte T: Will polymerase chain reaction (PCR)Ybased diagnostics improve outcome in septic patients? A clinical view. Intensive Care Med 37(7):1069-1076, 2011.
18. Ecker DJ, Sampath R, Li H, Massire C, Matthews HE, Toleno D, Hall TA, Blyn LB, Eshoo MW, Ranken R, et al.: New technology for rapid molecular diagnosis of bloodstream infections. Expert Rev Mol Diagn 10(4):399-415, 2010.
19. Sursal T, Stearns-Kurosawa DJ, Itagaki K, Oh SY, Sun S, Kurosawa S, Hauser CJ: Plasma bacterial and mitochondrial DNA distinguish bacterial sepsis from sterile systemic inflammation response syndrome and quantify inflammatory tissue injury in nonhuman primates. Shock 39:55-62, 2013.
20. Dark P, Wilson C, Blackwood B, McAuley DF, Perkins GD, McMullan R, Gates S, Warhurst G: Accuracy of LightCycler(R) SeptiFast for the detection and identification of pathogens in the blood of patients with suspected sepsis: a systematic review protocol. BMJ Open 2(1):1-6, 2012.
21. Dark P, Dunn G, Chadwick P, Young D, Bentley A, Carlson G, Warhurst G: The clinical diagnostic accuracy of rapid detection of healthcare-associated bloodstream infection in intensive care using multipathogen real-time PCR technology. BMJ Open 30(1):1-7, 2011.
22. Rello J, Lisboa T, Lujan M, Gallego M, Kee C, Kay I, Lopez D, Waterer GW; DNA-Pneumococo Study Group: Severity of pneumococcal pneumonia associated with genomic bacterial load. Chest 136(3):832-840, 2009.
23. Chang SS, Hsieh WH, Liu TS, Lee SH, Wang CH, Chou HC, Yeo YH, Tseng CP, Lee CC: Multiplex PCR system for rapid detection of pathogens in patients with presumed sepsisVa systemic review and meta-analysis. PLoS One 8(5):1-10, 2013.
24. Samraj RS, Zingarelli B, Wong HR: Role of biomarkers in sepsis care. Shock 40:358-365, 2013.
25. Andrade SS, Bispo PJ, gales AC: Advances in the microbiological diagnosis of sepsis. Shock 30(Suppl 1):41-46, 2008.
26. Palomares JC, Bernal S, Marín M, Holgado VP, Castro C, Morales WP, Martin E: Molecular diagnosis of Aspergillus fumigatus endocarditis. Diagn Microbiol Infect Dis 70(4):534-537, 2011.
27. Figueroa JR, Ortiz J, Morales I: Use of the LightCycler SeptiFast test for rapid etiologic diagnosis of nosocomial infection in gynecological sepsis. Gynecol Obstet Invest 70(3):215-216, 2010.
28. Steinmann J, Buer J, Rath PM, Paul A, Saner F: Invasive aspergillosis in two liver transplant recipients: diagnosis by SeptiFast. Transpl Infect Dis. 11(2):175-178, 2009.
29. Mencacci A, Leli C, Montagna P, Cardaccia A, Meucci M, Bietolini C, Cenci E, Pasticci MB, Bistoni F: Diagnosis of infective endocarditis: comparison of the LightCycler SeptiFast real-time PCR with blood culture. Clin Microbiol Infect 61:881-883, 2012.
30. Avolio M, Bonea M, Camporese A: Molecular diagnosis of Staphylococcus aureus prosthetic aortic graft infection: a case report. Infez Med. 20(4): 276-278, 2012.
31. Alvarez J, Mar J, Varela-Ledo E, Garea M, Matinez-Lamas L, Rodriguez J, Regueiro B: Cost analysis of real-time polymerase chain reaction microbiological diagnosis in patients with septic shock. Anaesth Intensive Care 40(6):958-963, 2012.