Isolation, culture and genetic manipulation of mouse pancreatic ductal cells

Nature Protocols

Volumn 8, Issue 7, 2013, Pages 1354-1365

  Reichert, Maximilian ^a,b   Takano, Shigetsugu ^a,b   Heeg, Steffen ^a,b,c   Bakir, Basil ^a,b   Botta, Gregory P ^a,d   Rustgi, Anil K ^a,b  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c UNIVERSITY OF FREIBURG   (Germany)

^d DREXEL UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DOLICHOS BIFLORUS AGGLUTININ LECTIN; LECTIN; LENTIVIRUS VECTOR; RNA; UNCLASSIFIED DRUG; DOLICHOS BIFLORUS AGGLUTININ; PLANT LECTIN;

ADULT; ANIMAL CELL; ARTICLE; CELL ASSAY; CELL CULTURE; CELL GROWTH; CELL ISOLATION; CELL LABELING; CELL LINEAGE; CELL SELECTION; CELL SUSPENSION; CELL VIABILITY; CLINICAL PROTOCOL; CONTROLLED STUDY; EMBRYO; EPITHELIUM CELL; GENE EXPRESSION; GENETIC MANIPULATION; IMMUNOFLUORESCENCE; MONOLAYER CULTURE; MOUSE; NONHUMAN; PANCREAS ADENOCARCINOMA; PANCREAS CYST; PANCREATIC DUCTAL EPITHELIAL CELL; PANCREATITIS; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; RNA ISOLATION; VIRAL GENE DELIVERY SYSTEM; ANIMAL; CELL SEPARATION; CHEMISTRY; CULTURE TECHNIQUE; CYTOLOGY; GENETIC TRANSDUCTION; GENETICS; LENTIVIRINAE; PANCREAS DUCT; PROCEDURES;

ANIMALS; CELL CULTURE TECHNIQUES; CELL SEPARATION; CELLS, CULTURED; GENE EXPRESSION; LENTIVIRUS; MICE; PANCREATIC DUCTS; PLANT LECTINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSDUCTION, GENETIC;

EID: 84904650619     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2013.079     Document Type: Article

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