Inner ear tissue preservation by rapid freezing: Improving fixation by high-pressure freezing and hybrid methods

Hearing Research

Volumn 315, Issue , 2014, Pages 49-60

  Bullen, A ^a   Taylor, R R ^a   Kachar, B ^b   Moores, C ^c   Fleck, R A ^d,e   Forge, A ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS   (United States)

^c UNIVERSITY OF LONDON   (United Kingdom)

^d NATIONAL INSTITUTE FOR BIOLOGICAL STANDARDS AND CONTROL   (United Kingdom)

^e KING'S COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; ARTIFACT REDUCTION; CILIATED EPITHELIUM; CONTROLLED STUDY; ELECTRON MICROSCOPY; INNER EAR; INTERMETHOD COMPARISON; MOUSE; NEUROEPITHELIUM; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; QUALITY CONTROL; RAPID FREEZE FIXATION; ROOM TEMPERATURE; TEMPERATURE SENSITIVITY; TISSUE PRESERVATION; ANIMAL; ANIMAL MODEL; ARTIFACT; C57BL MOUSE; CRYOPRESERVATION; GERBIL; GUINEA PIG; NOTOPHTHALMUS VIRIDESCENS; ORGAN PRESERVATION; PATHOLOGY; PROCEDURES; STEREOCILIUM; TIME; TISSUE FIXATION; ULTRASTRUCTURE;

ANIMALS; ARTIFACTS; CRYOPRESERVATION; EAR, INNER; GERBILLINAE; GUINEA PIGS; MICE; MICE, INBRED C57BL; MICROSCOPY, ELECTRON; MODELS, ANIMAL; NOTOPHTHALMUS VIRIDESCENS; ORGAN PRESERVATION; STEREOCILIA; TIME FACTORS; TISSUE FIXATION;

EID: 84904467287     PISSN: 03785955     EISSN: 18785891     Source Type: Journal    
DOI: 10.1016/j.heares.2014.06.006     Document Type: Article

References (42)

1. Al-Amoudi A., Chang J.J., Leforestier A., McDowall A., Salamin L.M., Norlen L.P., Richter K., Blanc N.S., Studer D., Dubochet J. Cryo-electron microscopy of vitreous sections. EMBO J. 2004, 23:3583-3588.
2. Boyde A., Boyde S. Further studies of specimen volume changes during processing for SEM: including some plant tissue. Scanning Electron Microsc. 1980, 117-124. 132.
3. Caulfield J.B. Effects of varying the vehicle for OsO4 in tissue fixation. J.Biophys. Biochem. Cytol. 1957, 3:827-830.
4. Claeys M., Vanhecke D., Couvreur M., Tytgat T., Coomans A., Borgonie G. High-pressure freezing and freeze substitution of gravid Caenorhabditis elegans (Nematoda: Rhabditida) for transmission electron microscopy. Nematology 2004, 6:319-327.
5. Dahl R., Staehelin L.A. High-pressure freezing for the preservation of biological structure: theory and practice. J.Electron Microsc. Tech. 1989, 13:165-174.
6. Derosier D.J., Tilney L.G., Egelman E. Actin in the inner-ear - the remarkable structure of the stereocilium. Nature 1980, 287:291-296.
7. Dubochet J. The Physics of Rapid Cooling and Its Implications for Cryoimmobilization of Cells 2007, vol. 79:7-21.
8. Dumont R.A., Lins U., Filoteo A.G., Penniston J.T., Kachar B., Gillespie P.G. Plasma membrane Ca2+-ATPase isoform 2a is the PMCA of hair bundles. J.Neurosci.: Off. J. Soc. Neurosci. 2001, 21:5066-5078.
9. Flock A., Cheung H.C. Actin-filaments in sensory hairs of inner-ear receptor cells. J.Cell. Biol. 1977, 75:339-343.
10. Forge A., Davies S., Zajic G. Assessment of ultrastructure in isolated cochlear hair-cells using a procedure for rapid freezing before freeze-fracture and deep-etching. J.Neurocytol. 1991, 20:471-484.
11. Galway M.E., Heckman J.W., Hyde G.J., Fowke L.C. Chapter 1 Advances in High-pressure And Plunge-freeze Fixation 1995, vol. 49:3-19.
12. Gilkey J.C., Staehelin L.A. Advances in ultra-rapid freezing for the preservation of cellular ultrastructure. J.Electron Microsc. Tech. 1986, 3:177-210.
13. Hayat M.A. Chemical fixation. Principles and Techniques of Electron Microscopy: Biological Applications 2000, 4-80. Cambridge University Press. fourth ed.
14. Hirokawa N., Tilney L.G. Interactions between actin-filaments and between actin-filaments and membranes in quick-frozen and deeply etched hair-cells of the chick ear. J.Cell. Biol. 1982, 95:249-261.
15. Kachar B., Parakkal M., Fex J. Structural basis for mechanical transduction in the frog vestibular sensory apparatus .1. The otolithic membrane. Hear. Res. 1990, 45:179-190.
16. Kachar B., Parakkal M., Kurc M., Zhao Y., Gillespie P.G. High-resolution structure of hair-cell tip links. Proc. Natl. Acad. Sci. U. S. A. 2000, 97:13336-13341.
17. Kellenberger E. The potential of cryofixation and freeze substitution: observations and theoretical considerations. J.Microsc. 1991, 161:183-203.
18. Koster A.J., Klumperman J. Electron microscopy in cell biology: integrating structure and function. Nat. Cell. Biol. 2003, Ss6-Ss10.
19. Leforestier A., Richter K., Livolant F., Dubochet J. Comparison of slam-freezing and high-pressure freezing effects on the DNA cholesteric liquid crystalline structure. J.Microsc. 1996, 184:4-13.
20. Lucic V., Rigort A., Baumeister W. Cryo-electron tomography: the challenge of doing structural biology in situ. J.Cell. Biol. 2013, 202:407-419.
21. McDonald K.L., Webb R.I. Freeze substitution in 3 hours or less. J.Microsc. 2011, 243:227-233.
22. McDonald K.L.M.,M., Verkade P., Muller-Reichert T. Recent advances in high-pressure freezing: equipment and specimen-loading methods. Electron Microscopy: Methods and Protocols 2007, 143-173. Humana Press. second ed. J. Kuo (Ed.).
23. Meissner D.H., Schwarz H. Improved cryoprotection and freeze-substitution of embryonic quail retina - a Tem study on ultrastructural preservation. J.Electron Microsc. Tech. 1990, 14:348-356.
24. Meyer A.C., Frank T., Khimich D., Hoch G., Riedel D., Chapochnikov N.M., Yarin Y.M., Harke B., Hell S.W., Egner A., Moser T. Tuning of synapse number, structure and function in the cochlea. Nat. Neurosci. 2009, 12:444-453.
25. Muller M. Cryopreparation of microorganisms for electron microscopy. Electron Microscopy in Microbiology 1988, 1-28. Academic Press Limited. F. Mayer (Ed.).
26. Ohta K., Higashi R., Sawaguchi A., Nakamura K. Helical arrangement of filaments in microvillar actin bundles. J.Struct. Biol. 2012, 177:513-519.
27. Resch G.P., Goldie K.N., Krebs A., Hoenger A., Small J.V. Visualisation of the actin cytoskelton by cryo-electron microscopy. J.Cell. Sci. 2002, 115:1877-1882.
28. Rzadzinska A.K., Schneider M.E., Davies C., Riordan G.P., Kachar B. An actin molecular treadmill and myosins maintain stereocilia functional architecture and self-renewal. J.Cell. Biol. 2004, 164:887-897.
29. Schneider C.A., Rasband W.S., Eliceiri K.W. NIH image to IMAGEJ: 25 years of image analysis. Nat. Methods 2012, 9:671-675.
30. Semmler K., Wunderlich J., Richter W., Meyer H.W. High-pressure freezing causes structural alterations in phospholipid model membranes. J.Microsc-Oxford 1998, 190:317-327.
31. Small J.V. Organization of actin in the leading edge of cultured cells: influence of osmium tetroxide and dehydration on the ultrastructure of actin meshworks. J.Cell. Biol. 1981, 91:695-705.
32. Small J.V., Rottner K., Hahne P., Anderson K.I. Visualising the actin cytoskeleton. Microsc. Res. Tech. 1999, 47:3-17.
33. Sosinsky G.E., Deerinck T.J., Greco R., Buitenhuys C.H., Bartol T.M., Ellisman M.H. Development of a model for microphysiological simulations: small nodes of ranvier from peripheral nerves of mice reconstructed by electron tomography. Neuroinformatics 2005, 3:133-162.
34. Sosinsky G.E., Crum J., Jones Y.Z., Lanman J., Smarr B., Terada M., Martone M.E., Deerinck T.J., Johnson J.E., Ellisman M.H. The combination of chemical fixation procedures with high pressure freezing and freeze substitution preserves highly labile tissue ultrastructure for electron tomography applications. J.Struct. Biol. 2008, 161:359-371.
35. Steinbrecht R.A. Recrystallization and ice-crystal growth in a biological specimen, as shown by a simple freeze substitution method. J.Microsc-Oxford 1985, 140:41-46.
36. Studer D., Hennecke H., Muller M. High-pressure freezing of soybean nodules leads to an improved preservation of ultrastructure. Planta 1992, 188:155-163.
37. Studer D., Humbel B.M., Chiquet M. Electron microscopy of high pressure frozen samples: bridging the gap between cellular ultrastructure and atomic resolution. Histochem. Cell. Biol. 2008, 130:877-889.
38. Studer D., Graber W., Al-Amoudi A., Eggli P. Anew approach for cryofixation by high-pressure freezing. J.Microsc. 2001, 203:285-294.
39. Studer D., Michel M., Wohlwend M., Hunziker E.B., Buschmann M.D. Vitrification of articular-cartilage by high-pressure freezing. J.Microsc. 1995, 179:321-332.
40. Taylor R.R., Forge A. Hair cell regeneration in sensory epithelia from the inner ear of a urodele amphibian. J.Comp. Neurol. 2005, 484:105-120.
41. Tilney L.G., Connelly P.S., Vranich K.A., Shaw M.K., Guild G.M. Why are two different cross-linkers necessary for actin bundle formation invivo and what does each cross-link contribute?. J.Cell. Biol. 1998, 143:121-133.
42. White D.L., Andrews S.B., Faller J.W., Barrnett R.J. Chemical nature of osmium tetroxide fixation and staining of membranes by X-Ray photoelectron-spectroscopy. Biochim. Biophys. Acta 1976, 436:577-592.