High-yield expression in Escherichia coli, purification and application of budding yeast K2 killer protein

Molecular Biotechnology

Volumn 56, Issue 7, 2014, Pages 644-652

  Podoliankaitė, Monika ^a   Lukša, Juliana ^a   Vyšniauskis, Gintautas ^a,b   Sereikaitė, Jolanta ^c   Melvydas, Vytautas ^a   Serva, Saulius ^c,d   Servienė, Elena ^a,c  

^a Nature Research Centre   (Lithuania)

^b State Research Institute Center for Innovative Medicine   (Lithuania)

^c VILNIUS GEDIMINAS TECHNICAL UNIVERSITY   (Lithuania)

^d VILNIUS UNIVERSITY   (Lithuania)

Author keywords

Antibody; Escherichia coli; K2 recombinant protein; K2 toxin; Saccharomyces cerevisiae

Indexed keywords

ANTIBODIES; BIOCONTROL; CHEMICAL DETECTION; ESCHERICHIA COLI; PURIFICATION; TOXIC MATERIALS; YEAST;

BACTERIAL SYSTEMS; BIOTECHNOLOGICAL APPLICATIONS; C-TERMINAL TRUNCATION; EXPRESSION IN ESCHERICHIA COLI; EXTRACELLULAR PROTEINS; K2 TOXIN; POLYCLONAL ANTIBODY; PROTEIN AGGREGATES;

RECOMBINANT PROTEINS;

K2 PROTEIN; MYCOTOXIN; POLYCLONAL ANTIBODY; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; ANTIBODY; K2 KILLER TOXIN; YEAST KILLER FACTOR;

ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ANTIBODY SPECIFICITY; ANTIGEN SPECIFICITY; ARTICLE; BACTERIAL GROWTH; CARBOXY TERMINAL SEQUENCE; CELL INCLUSION; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; HOST CELL; NONHUMAN; PROTEIN AGGREGATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN REFOLDING; SACCHAROMYCES CEREVISIAE; SOLUBILITY; AMINO ACID SEQUENCE; ANIMAL; BIOSYNTHESIS; GENE EXPRESSION REGULATION; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; MOLECULAR CLONING; RABBIT;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; ORYCTOLAGUS CUNICULUS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, FUNGAL; KILLER FACTORS, YEAST; RABBITS; SACCHAROMYCES CEREVISIAE;

EID: 84904288322     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-014-9740-6     Document Type: Article

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