Strategies for optimal expression of vaccine antigens from taeniid cestode parasites in escherichia coli

Molecular Biotechnology

Volumn 48, Issue 3, 2011, Pages 277-289

  Gauci, Charles ^a   Jenkins, David ^b   Lightowlers, Marshall W ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

^b CHARLES STURT UNIVERSITY   (Australia)

Author keywords

Antigen expression; Cysticercosis; Echinococcus; Escherichia; Parasite; Recombinant; Taenia; Vaccine

Indexed keywords

ANTIGEN EXPRESSION; CYSTICERCOSIS; ECHINOCOCCUS; ESCHERICHIA; PARASITE-; RECOMBINANT; TAENIA;

AMINO ACIDS; DNA; ESCHERICHIA COLI; HYDROPHOBICITY; OPTIMIZATION; PROTEINS; VACCINES;

ANTIGENS;

FIBRONECTIN; RECOMBINANT ANTIGEN; VACCINE; EG95 PROTEIN, ECHINOCOCCUS GRANULOSUS; HELMINTH PROTEIN; HYBRID PROTEIN; PARASITE ANTIGEN; RECOMBINANT PROTEIN; RECOMBINANT VACCINE; TSOL 18 ANTIGEN; TSOL-18 ANTIGEN;

AMINO TERMINAL SEQUENCE; ANTIGEN EXPRESSION; ANTIGENICITY; ARTICLE; CARBOXY TERMINAL SEQUENCE; CESTODE; CONTROLLED STUDY; ECHINOCOCCUS GRANULOSUS; ESCHERICHIA COLI; GENETIC MANIPULATION; HETEROLOGOUS EXPRESSION; HYDROPHOBICITY; NONHUMAN; PLASMID; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; TAENIA SOLIUM; AMINO ACID SEQUENCE; ANIMAL; BIOSYNTHESIS; CHEMISTRY; GENETICS; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NONPARAMETRIC TEST; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ENGINEERING; TAENIA; WESTERN BLOTTING;

BACTERIA (MICROORGANISMS); CESTODA; ECHINOCOCCUS; ECHINOCOCCUS GRANULOSUS; ESCHERICHIA; ESCHERICHIA COLI; TAENIA; TAENIA MULTICEPS; TAENIA SOLIUM; TAENIIDAE;

AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, HELMINTH; BLOTTING, WESTERN; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HELMINTH PROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; STATISTICS, NONPARAMETRIC; TAENIA; VACCINES, SYNTHETIC;

EID: 80054054091     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-010-9368-0     Document Type: Article

References (40)

1. Harrison, G. B., Heath, D. D., Dempster, R. P., Gauci, C., Newton, S. E., Cameron, W. G., et al. (1996). Identification and cDNA cloning of two novel low molecular weight host-protective antigens from Taenia ovis oncospheres. International Journal for Parasitology, 26, 195-204. (Pubitemid 26074225)
2. Johnson, K. S., Harrison, G. B., Lightowlers, M. W., O'Hoy, K. L., Cougle, W. G., Dempster, R. P., et al. (1989). Vaccination against ovine cysticercosis using a defined recombinant antigen. Nature, 338, 585-587. (Pubitemid 19102310)
3. Lightowlers, M. W., Rolfe, R., & Gauci, C. G. (1996). Taenia saginata: Vaccination against cysticercosis in cattle with recombinant oncosphere antigens. Experimental Parasitology, 84, 330-338. (Pubitemid 26412994)
4. Flisser, A., Gauci, C. G., Zoli, A., Martinez-Ocana, J., Garza-Rodriguez, A., Dominguez-Alpizar, J. L., et al. (2004). Induction of protection against porcine cysticercosis by vaccination with recombinant oncosphere antigens. Infection and Immunity, 72, 5292-5297. (Pubitemid 39223416)
5. Gonzalez, A. E., Gauci, C. G., Barber, D., Gilman, R. H., Tsang, V. C., Garcia, H. H., et al. (2005). Vaccination of pigs to control human neurocysticercosis. American Journal of Tropical Medicine and Hygiene, 72, 837-839. (Pubitemid 41278809)
6. Gauci, C., Vural, G., Oncel, T., Varcasia, A., Damian, V., Kyngdon, C. T., et al. (2008). Vaccination with recombinant oncosphere antigens reduces the susceptibilityofsheep toinfection with Taenia multiceps. International Journal for Parasitology, 38, 1041-1050.
7. Lightowlers, M. W., Lawrence, S. B., Gauci, C. G., Young, J., Ralston, M. J., Maas, D., et al. (1996). Vaccination against hydatidosis using a defined recombinant antigen. Parasite Immunology, 18, 457-462. (Pubitemid 26302819)
8. Geldhof, P., De Maere, V., Vercruysse, J., & Claerebout, E. (2007). Recombinant expression systems: The obstacle to helminth vaccines? Trends in Parasitology, 23, 527-532. (Pubitemid 350026469)
9. Smith, D. B., & Johnson, K. (1988). Single-step purification of polypeptides expressed in Escherichia coli as fusion proteins with glutathione S-transferase. Gene, 67, 31-40.
10. Lightowlers, M. W., Waterkeyn, J. G., Rothel, J. S., Gauci, C. G., & Harrison, G. B. (1996). Host-protective fragments and antibody binding epitopes of the Taenia ovis 45W recombinant antigen. Parasite Immunology, 18, 507-513. (Pubitemid 26342889)
11. Dempster, R. P., Robinson, C. M., & Harrison, G. B. (1996). Parasite vaccine development: Large-scale recovery of immunogenic Taenia ovis fusion protein GST-45W(B/X) from Escherichia coli inclusion bodies. Parasitology Research, 82, 291-296. (Pubitemid 26145282)
12. Manderson, D., Dempster, R., & Chisti, Y. (2006). A recombi-nant vaccine against hydatidosis: Production of the antigen in Escherichia coli. Journal of Industrial Microbiology and Biotechnology, 33, 173-182. (Pubitemid 43288306)
13. Cabrita, L. D., & Bottomley, S. P. (2004). Protein expression and refolding-A practical guide to getting the most out of inclusion bodies. Biotechnology Annual Review, 10, 31-50. (Pubitemid 39419988)
14. Gauci, C. G., Flisser, A., & Lightowlers, M. W. (1998). A Taenia solium oncosphere protein homologous to host-protective Taenia ovis and Taenia saginata 18 kDa antigens. International Journal for Parasitology, 28, 757-760. (Pubitemid 28266905)
15. Chow, C., Gauci, C. G., Cowman, A. F., & Lightowlers, M. W. (2001). A gene family expressing a host-protective antigen of Echinococcus granulosus. Molecular and Biochemical Parasi-tology, 118, 83-88. (Pubitemid 33043823)
16. Lilius, G., Persson, M., Bulow, L., & Mosbach, K. (1991). Metal affinity precipitation of proteins carrying genetically attached polyhistidine affinity tails. European Journal of Biochemistry, 198, 499-504.
17. Stuber, D., Matile, H., & Garotta, G. (1990). System for high-level production in Escherichia coli and rapid purification of recombi-nant proteins: Application to epitope mapping, preparation of antibodies and structure-function analysis. In I. Lefkovits & B. Pernis (Eds.), Immunological methods (Vol. 4, pp. 121-152). New York: Academic Press.
18. Bradford, M. M. (1976). A rapid and sensitive method for the quantitationofmicrogram quantities ofprotein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.
19. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
20. Bendtsen, J. D., Nielsen, H., von Heijne, G., & Brunak, S. (2004). Improved prediction of signal peptides: SignalP 3.0. Journal of Molecular Biology, 340, 783-795. (Pubitemid 38829638)
21. Ponting, C. P., Schultz, J., Milpetz, F., & Bork, P. (1999). SMART: Identification and annotation of domains from signalling and extracellular protein sequences. Nucleic Acids Research, 27, 229-232. (Pubitemid 29209446)
22. Hofmann, K., & Stoffel, W. (1993). TMbase-A database of membrane spanning proteins segments. Biological Chemistry, 374, 166.
23. Krogh, A., Larsson, B., von Heijne, G., & Sonnhammer, E. L. (2001). Predicting transmembrane protein topology with a hidden Markov model: Application to complete genomes. Journal of Molecular Biology, 305, 567-580. (Pubitemid 33032862)
24. Grossman, T. H., Kawasaki, E. S., Punreddy, S. R., & Osburne, M. S. (1998). Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability. Gene, 209, 95-103. (Pubitemid 28149625)
25. Pan, S. H., & Malcolm, B. A. (2000). Reduced background expression and improved plasmid stability with pET vectors in BL21 (DE3). BioTechniques, 29, 1234-1238. (Pubitemid 32002630)
26. Lee, S. Y. (1996). High cell-density culture of Escherichia coli. Trends in Biotechnology, 14, 98-105. (Pubitemid 26079509)
27. Schein, C. H., & Noteborn, M. H. (1988). Formation of soluble recombinant proteins in Escherichia coli is favored by lower growth temperature. Biotechnology, 6, 291-294.
28. Vera, A., Gonzalez-Montalban, N., Aris, A., & Villaverde, A. (2007). The conformational quality of insoluble recombinant proteins is enhanced at low growth temperatures. Biotechnology and Bioengineering, 96, 1101-1106. (Pubitemid 46648670)
29. Sorensen, H. P., & Mortensen, K. K. (2005). Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli. Microbial Cell Factories, 4, 1.
30. Woollard, D. J., Gauci, C. G., Heath, D. D., & Lightowlers, M. W. (2001). Protection against hydatid disease induced with the EG95 vaccine is associated with conformational epitopes. Vaccine, 19, 498-507.
31. Lightowlers, M. W. (2006). Cestode vaccines: Origins, current status and future prospects. Parasitology, 133 Suppl, S27-S42. (Pubitemid 46208029)
32. Puigbo, P., Guzman, E., Romeu, A., & Garcia-Vallve, S. (2007). OPTIMIZER: A web server for optimizing the codon usage of DNA sequences. Nucleic Acids Research, 35, W126-W131.
33. Gustafsson, C., Govindarajan, S., & Minshull, J. (2004). Codon bias and heterologous protein expression. Trends in Biotechnology, 22, 346-353. (Pubitemid 38887543)
34. Mehlin, C., Boni, E., Buckner, F. S., Engel, L., Feist, T., Gelb, M. H., et al. (2006). Heterologous expression of proteins from Plasmodium falciparum: Results from 1000 genes. Molecular and Biochemical Parasitology, 148, 144-160. (Pubitemid 43964865)
35. Griswold, K. E., Mahmood, N. A., Iverson, B. L., & Georgiou, G. (2003). Effects of codon usage versus putative 5-mRNA structure on the expression of Fusarium solani cutinase in the Esch-erichia coli cytoplasm. Protein Expression and Purification, 27, 134-142. (Pubitemid 37096479)
36. Wu, X., Jornvall, H., Berndt, K. D., & Oppermann, U. (2004). Codon optimization reveals critical factors for high level expression of two rare codon genes in Escherichia coli: RNA stability and secondary structure but not tRNA abundance. Biochemical and Biophysical Research Communication, 313, 89-96. (Pubitemid 37524029)
37. Varcasia, A., Tosciri, G., Coccone, G. N., Pipia, A. P., Garippa, G., Scala, A., et al. (2009). Preliminary field trial of a vaccine against coenurosis caused by Taenia multiceps. Veterinary Parasitology, 162, 285-289.
38. Scala, A., & Varcasia, A. (2006). Updates on morphobiology, epidemiology and molecular characterization of coenurosis in sheep. Parassitologia, 48, 61-63. (Pubitemid 44378845)
39. Sharma, D. K., & Chauhan, P. P. S. (2006). Coenurosis status in Afro-Asian region: A review. Small Ruminant Research, 64, 197.
40. Lightowlers, M. (2010). Eradication of Taenia solium cysticer-cosis: A role for vaccination of pigs. International Journal for Parasitology, 40, 1183-1192.