Integration of cell lysis, protein extraction, and digestion into one step for ultrafast sample preparation for phosphoproteome analysis

Analytical Chemistry

Volumn 86, Issue 14, 2014, Pages 6786-6791

  Liu, Fangjie ^a,b   Ye, Mingliang ^a   Pan, Yanbo ^a,b   Zhang, Yi ^a,b   Bian, Yangyang ^a,b   Sun, Zhen ^a,b   Zhu, Jun ^a,b   Cheng, Kai ^a,b   Zou, Hanfa ^a  

^a DALIAN INSTITUTE OF CHEMICAL PHYSICS   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOLOGY; EXTRACTION; PEPTIDES;

BROAD APPLICATION; CONVENTIONAL METHODS; PEPTIDE MIXTURES; PHOSPHOPEPTIDE ENRICHMENT; PHOSPHOPROTEOMICS; PROTEIN DIGESTION; PROTEIN EXTRACTION; SAMPLE PREPARATION;

CELLS;

BUFFER; PHOSPHOPROTEIN; TRYPSIN;

CHEMISTRY; FRACTIONATION; HELA CELL LINE; HUMAN; PROCEDURES; PROTEOMICS; SOLUBILITY; TIME;

BUFFERS; CHEMICAL FRACTIONATION; HELA CELLS; HUMANS; PHOSPHOPROTEINS; PROTEOMICS; SOLUBILITY; TIME FACTORS; TRYPSIN;

EID: 84904279815     PISSN: 00032700     EISSN: 15206882     Source Type: Journal    
DOI: 10.1021/ac5002146     Document Type: Article

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