Knockdown of EpCAM enhances the chemosensitivity of breast cancer cells to 5-fluorouracil by downregulating the antiapoptotic factor Bcl-2

PLoS ONE

Volumn 9, Issue 7, 2014, Pages

  Gao, Jiujiao ^a   Yan, Qiu ^a   Liu, Shuai ^a   Yang, Xuesong ^a  

^a DALIAN MEDICAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CASPASE 3; EPITHELIAL CELL ADHESION MOLECULE; FLUOROURACIL; PROTEIN BCL 2; ANTINEOPLASTIC AGENT; BAX PROTEIN, HUMAN; CELL ADHESION MOLECULE; PROTEIN BAX; TUMOR ANTIGEN; TUMOR-ASSOCIATED ANTIGEN GA733;

ANIMAL CELL; ANTINEOPLASTIC ACTIVITY; APOPTOSIS; ARTICLE; BREAST CANCER; CANCER CELL; CELL CYCLE ARREST; CELL CYCLE G1 PHASE; CELL CYCLE PROGRESSION; CELL CYCLE S PHASE; CELL PROLIFERATION; CELL SURVIVAL; CELL VIABILITY; CHEMOSENSITIVITY; CONCENTRATION RESPONSE; DOWN REGULATION; DRUG CYTOTOXICITY; DRUG EFFICACY; DRUG MECHANISM; EPCAM GENE; G2 PHASE CELL CYCLE CHECKPOINT; GENE EXPRESSION; GENE TARGETING; HUMAN; HUMAN CELL; NONHUMAN; ONCOGENE; PROTEIN EXPRESSION; UPREGULATION; BREAST TUMOR; CELL CYCLE CHECKPOINT; DRUG EFFECTS; DRUG RESISTANCE; FEMALE; GENE EXPRESSION REGULATION; GENE SILENCING; GENETICS; MCF 7 CELL LINE; METABOLISM; PATHOLOGY; PHYSIOLOGY; SIGNAL TRANSDUCTION; TUMOR CELL LINE;

ANTIGENS, NEOPLASM; ANTINEOPLASTIC AGENTS; APOPTOSIS; BCL-2-ASSOCIATED X PROTEIN; BREAST NEOPLASMS; CASPASE 3; CELL ADHESION MOLECULES; CELL CYCLE CHECKPOINTS; CELL LINE, TUMOR; CELL PROLIFERATION; DOWN-REGULATION; DRUG RESISTANCE, NEOPLASM; FEMALE; FLUOROURACIL; GENE EXPRESSION REGULATION, NEOPLASTIC; GENE KNOCKDOWN TECHNIQUES; HUMANS; MAP KINASE SIGNALING SYSTEM; MCF-7 CELLS; SIGNAL TRANSDUCTION;

EID: 84904248536     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0102590     Document Type: Article

References (37)

1. Jemal A, Bray F, Center MM, Ferlay J, Ward E, et al. (2011) Global cancer statistics. CA Cancer J Clin 61: 69-90.
2. Ohno S, Chow LW, Sato N, Masuda N, Sasano H, et al. (2013) Randomized trial of preoperative docetaxel with or without capecitabine after 4 cycles of 5-fluorouracil-epirubicin-cyclophosphamide (FEC) in early-stage breast cancer: exploratory analyses identify Ki67 as a predictive biomarker for response to neoadjuvant chemotherapy. Breast Cancer Res Treat.
3. Chow LW, Tung SY, Ng TY, Im SA, Lee MH, et al. (2013) Concurrent celecoxib with 5-fluorouracil/epirubicin/cyclophosphamide followed by docetaxel for stages II - III invasive breast cancer: the OOTR-N001 study. Expert Opin Investig Drugs 22: 299-307.
4. Russo F, Linsalata M, Clemente C, D'Attoma B, Orlando A, et al. (2013) The effects of fluorouracil, epirubicin, and cyclophosphamide (FEC60) on the intestinal barrier function and gut peptides in breast cancer patients: an observational study. BMC Cancer 13: 56.
5. Qu C, Zhang W, Zheng G, Zhang Z, Yin J, et al. (2013) Metformin reverses multidrug resistance and epithelial-mesenchymal transition (EMT) via activating AMP-activated protein kinase (AMPK) in human breast cancer cells. Mol Cell Biochem.
6. Takahashi K, Tanaka M, Inagaki A, Wanibuchi H, Izumi Y, et al. (2013) Establishment of a 5-fluorouracil-resistant triple-negative breast cancer cell line. Int J Oncol 43: 1985-1991.
7. Imrich S, Hachmeister M, Gires O (2012) EpCAM and its potential role in tumor-initiating cells. Cell Adh Migr 6: 30-38.
8. van der Gun BT, Melchers LJ, Ruiters MH, de Leij LF, McLaughlin PM, et al. (2010) EpCAM in carcinogenesis: the good, the bad or the ugly. Carcinogenesis 31: 1913-1921.
9. Gostner JM, Fong D, Wrulich OA, Lehne F, Zitt M, et al. (2011) Effects of EpCAM overexpression on human breast cancer cell lines. BMC Cancer 11: 45.
10. Ni J, Cozzi P, Hao J, Beretov J, Chang L, et al. (2013) Epithelial cell adhesion molecule (EpCAM) is associated with prostate cancer metastasis and chemo/radioresistance via the PI3K/Akt/mTOR signaling pathway. Int J Biochem Cell Biol 45: 2736-2748.
11. Bugalho A, Martins C, Dias SS, Nunes G, Silva Z, et al. (2013) Cytokeratin 19, Carcinoembryonic Antigen, and Epithelial Cell Adhesion Molecule Detect Lung Cancer Lymph Node Metastasis in Endobronchial Ultrasound-Guided Transbronchial Aspiration Samples. Clin Lung Cancer.
12. Garcia-Saez AJ (2012) The secrets of the Bcl-2 family. Cell Death Differ 19: 1733-1740.
13. Fu Q, He C, Mao ZR (2013) Epstein-Barr virus interactions with the Bcl-2 protein family and apoptosis in human tumor cells. J Zhejiang Univ Sci B 14: 8-24.
14. Kelly PN, Strasser A (2011) The role of Bcl-2 and its pro-survival relatives in tumourigenesis and cancer therapy. Cell Death Differ 18: 1414-1424.
15. Hardwick JM, Chen YB, Jonas EA (2012) Multipolar functions of BCL-2 proteins link energetics to apoptosis. Trends Cell Biol 22: 318-328.
16. Fan Y, Wang S, Hernandez J, Yenigun VB, Hertlein G, et al. (2014) Genetic models of apoptosis-induced proliferation decipher activation of JNK and identify a requirement of EGFR signaling for tissue regenerative responses in Drosophila. PLoS Genet 10: e1004131.
17. Junttila MR, Li SP, Westermarck J (2008) Phosphatase-mediated crosstalk between MAPK signaling pathways in the regulation of cell survival. FASEB J 22: 954-965. (Pubitemid 351519953)
18. Nunna S, Reinhardt R, Ragozin S, Jeltsch A (2014) Targeted Methylation of the Epithelial Cell Adhesion Molecule (EpCAM) Promoter to Silence Its Expression in Ovarian Cancer Cells. PLoS One 9: e87703.
19. Lund K, Bostad M, Skarpen E, Braunagel M, Krauss S, et al. (2014) The novel EpCAM-targeting monoclonal antibody 3-17I linked to saporin is highly cytotoxic after photochemical internalization in breast, pancreas and colon cancer cell lines. MAbs 6.
20. Ogawa K, Tanaka S, Matsumura S, Murakata A, Ban D, et al. (2014) EpCAMTargeted Therapy for Human Hepatocellular Carcinoma. Ann Surg Oncol 21: 1314-1322.
21. Liljefors M, Ragnhammar P, Nilsson B, Ullenhag G, Mellstedt H, et al. (2004) Anti-EpCAM monoclonal antibody (MAb17-1A) based treatment combined with alpha-interferon, 5-fluorouracil and granulocyte-macrophage colonystimulating factor in patients with metastatic colorectal carcinoma. Int J Oncol 25: 703-711.
22. Flatmark K, Guldvik IJ, Svensson H, Fleten KG, Florenes VA, et al. (2013) Immunotoxin targeting EpCAM effectively inhibits peritoneal tumor growth in experimental models of mucinous peritoneal surface malignancies. Int J Cancer 133: 1497-1506.
23. Ayyagari VN, Brard L (2014) Bithionol inhibits ovarian cancer cell growth in vitro - studies on mechanism(s) of action. BMC Cancer 14: 61.
24. He L, Lu N, Dai Q, Zhao Y, Zhao L, et al. (2013) Wogonin induced G1 cell cycle arrest by regulating Wnt/beta-catenin signaling pathway and inactivating CDK8 in human colorectal cancer carcinoma cells. Toxicology 312: 36-47.
25. Sorrentino MF, Kim J, Foderaro AE, Truesdell AG (2012) 5-fluorouracil induced cardiotoxicity: review of the literature. Cardiol J 19: 453-458.
26. Longley DB, Harkin DP, Johnston PG (2003) 5-fluorouracil: mechanisms of action and clinical strategies. Nat Rev Cancer 3: 330-338. (Pubitemid 37328853)
27. Ferguson JE, Orlando RA (2014) Curcumin Reduces Cytotoxicity of 5- Fluorouracil Treatment in Human Breast Cancer Cells. J Med Food.
28. Yang D, Chen MB, Wang LQ, Yang L, Liu CY, et al. (2013) Bcl-2 expression predicts sensitivity to chemotherapy in breast cancer: a systematic review and meta-analysis. J Exp Clin Cancer Res 32: 105.
29. Moldoveanu T, Follis AV, Kriwacki RW, Green DR (2014) Many players in BCL-2 family affairs. Trends Biochem Sci 39: 101-111.
30. Czabotar PE, Lessene G, Strasser A, Adams JM (2014) Control of apoptosis by the BCL-2 protein family: implications for physiology and therapy. Nat Rev Mol Cell Biol 15: 49-63.
31. Geng M, Wang L, Li P (2013) Correlation between chemosensitivity to anticancer drugs and Bcl-2 expression in gastric cancer. Int J Clin Exp Pathol 6: 2554-2559.
32. Geng ZM, Zhang M, Pan XT, Wang L (2013) Bcl-2 gene silencing by RNA interference inhibits the growth of the human gallbladder carcinoma cell line GBC-SD in vitro and in vivo. Oncol Rep 30: 793-800.
33. Hall C, Troutman SM, Price DK, Figg WD, Kang MH (2013) Bcl-2 family of proteins as therapeutic targets in genitourinary neoplasms. Clin Genitourin Cancer 11: 10-19.
34. Barille-Nion S, Bah N, Vequaud E, Juin P (2012) Regulation of cancer cell survival by BCL2 family members upon prolonged mitotic arrest: opportunities for anticancer therapy. Anticancer Res 32: 4225-4233.
35. Plotnikov A, Zehorai E, Procaccia S, Seger R (2011) The MAPK cascades: signaling components, nuclear roles and mechanisms of nuclear translocation. Biochim Biophys Acta 1813: 1619-1633.
36. Kim EK, Choi EJ (2010) Pathological roles of MAPK signaling pathways in human diseases. Biochim Biophys Acta 1802: 396-405.
37. Liu J, Lin A (2005) Role of JNK activation in apoptosis: a double-edged sword. Cell Res 15: 36-42. (Pubitemid 41653963)