Development of an activity-directed selection system enabled significant improvement of the carboxylation efficiency of Rubisco

Protein and Cell

Volumn 5, Issue 7, 2014, Pages 552-562

  Cai, Zhen ^a   Liu, Guoxia ^a   Zhang, Junli ^b   Li, Yin ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

Author keywords

carboxylation efficiency; directed evolution; Rubisco; Synechococcus sp. PCC7002

Indexed keywords

HOLOENZYME; RIBULOSEBISPHOSPHATE CARBOXYLASE; BACTERIAL PROTEIN; CARBON DIOXIDE;

ARTICLE; CARBOXYLATION; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME PURIFICATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE MUTATION; MOLECULAR LIBRARY; MUTANT; NONHUMAN; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; SYNECHOCOCCUS ELONGATUS; WESTERN BLOTTING; AMINO ACID SUBSTITUTION; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; ENZYMOLOGY; GENETICS; GROWTH, DEVELOPMENT AND AGING; SYNECHOCOCCUS;

CHLAMYDOMONAS REINHARDTII; EMBRYOPHYTA; ESCHERICHIA COLI; SYNECHOCOCCUS SP.;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; CARBON DIOXIDE; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; RIBULOSE-BISPHOSPHATE CARBOXYLASE; SYNECHOCOCCUS;

EID: 84904190583     PISSN: 1674800X     EISSN: 16748018     Source Type: Journal    
DOI: 10.1007/s13238-014-0072-x     Document Type: Article

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