Alanine-scanning mutagenesis of the small-subunit βA - βB loop of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase: Substitution at Arg-71 affects thermal stability and CO2/O2 specificity

Biochemistry

Volumn 40, Issue 19, 2001, Pages 5615-5621

  Spreitzer, Robert J ^a   Esquivel, Maria G ^b   Du, Yu Chun ^a   McLaughlin, Patrick D ^a  

^a UNIVERSITY OF NEBRASKA LINCOLN   (United States)

^b UNIVERSITY OF LISBON   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

ALGAE; CARBON MONOXIDE; CARBOXYLATION; CATALYSIS; GENES; MUTAGENESIS;

RUBISCO STRUCTURE;

ENZYMES;

RIBULOSEBISPHOSPHATE CARBOXYLASE;

AMINO ACID SUBSTITUTION; ARTICLE; CARBOXYLATION; CATALYSIS; CHLAMYDOMONAS REINHARDTII; CHLOROPLAST; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; NONHUMAN; PRIORITY JOURNAL; THERMOSTABILITY;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; ARGININE; CARBON DIOXIDE; CHLAMYDOMONAS REINHARDTII; CHLOROPLASTS; ENZYME STABILITY; HEAT; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OXYGEN; PHENOTYPE; PROTEIN STRUCTURE, SECONDARY; RIBULOSE-BISPHOSPHATE CARBOXYLASE; SUBSTRATE SPECIFICITY; TRANSFORMATION, GENETIC;

ALGAE; CHLAMYDOMONAS REINHARDTII; CHLOROPHYTA; EMBRYOPHYTA; PROKARYOTA;

EID: 0035873032     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi002943e     Document Type: Article

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