The Glu216/Ser218 pocket is a major determinant of spermine oxidase substrate specificity

Biochemical Journal

Volumn 461, Issue 3, 2014, Pages 453-459

  Cervelli, Manuela ^a,b   Angelucci, Emanuela ^a   Stano, Pasquale ^a   Leboffe, Loris ^a   Federico, Rodolfo ^a   Antonini, Giovanni ^a,b   Mariottini, Paolo ^a,b   Polticelli, Fabio ^a,c  

^a ROMA TRE UNIVERSITY   (Italy)

^b Interuniversity Consortium of Structural and Systems Biology   (Italy)

^c INFN SEZIONE DI ROMA   (Italy)

Author keywords

Acetylpolyamine oxidase (APAO); Enzyme catalysis; Enzyme kinetics; Polyamine; Spermine oxidase (SMO); Substrate specificity

Indexed keywords

ACETYLPOLYAMINE OXIDASE; FLAVOPROTEIN; GLUTAMIC ACID; OXIDOREDUCTASE; POLYAMINE; SERINE; SPERMINE; SPERMINE OXIDASE; UNCLASSIFIED DRUG; DRUG DERIVATIVE; MUTANT PROTEIN; N' ACETYLSPERMINE; N'-ACETYLSPERMINE; POLYAMINE OXIDASE; RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

ARTICLE; CATABOLISM; CATALYSIS; CELL GROWTH; CELL PROLIFERATION; CIRCULAR DICHROISM; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; GENE MUTATION; HOMEOSTASIS; KINETICS; MOUSE; MUTANT; NEOPLASM; NONHUMAN; OXIDATION; POLYAMINE METABOLISM; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STEADY STATE; VERTEBRATE; AMINO ACID SUBSTITUTION; ANIMAL; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME ACTIVE SITE; ENZYMOLOGY; GENETICS; MAIZE; METABOLISM; OXIDATION REDUCTION REACTION; PROTEIN CONFORMATION;

AMINO ACID SUBSTITUTION; ANIMALS; CATALYTIC DOMAIN; CIRCULAR DICHROISM; GLUTAMIC ACID; KINETICS; MICE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; OXIDATION-REDUCTION; OXIDOREDUCTASES ACTING ON CH-NH GROUP DONORS; PLANT PROTEINS; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SERINE; SPERMINE; SUBSTRATE SPECIFICITY; ZEA MAYS;

EID: 84904094118     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20140305     Document Type: Article

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