A real-time high-throughput fl uorescence assay for sphingosine kinases

Journal of Lipid Research

Volumn 55, Issue 7, 2014, Pages 1525-1530

  Lima, Santiago ^a   Milstien, Sheldon ^a   Spiegel, Sarah ^a  

^a VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

Author keywords

Fl uorescence sphingosine kinase assay; Nbd sphingosine; Red emission excitation shift; Sphingosine kinase 1; Sphingosine kinase 2

Indexed keywords

4 CHLORO 7 NITROBENZOFURAZAN; ADENOSINE TRIPHOSPHATE; ISOENZYME; RADIOACTIVE MATERIAL; RECOMBINANT ENZYME; SPHINGOSINE KINASE; SPHINGOSINE KINASE 1; SPHINGOSINE KINASE 2; FLUORESCENT DYE; PHOSPHOTRANSFERASE; SPHINGOSINE; SPHINGOSINE KINASE 2, HUMAN;

ARTICLE; BINDING AFFINITY; CATALYSIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SUBSTRATE; FLUORESCENCE ANALYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; HYDROLYSIS; IC 50; PRIORITY JOURNAL; CHEMISTRY; FLUORESCENCE; HUMAN;

FLUORESCENCE; FLUORESCENT DYES; HUMANS; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); SPHINGOSINE;

EID: 84903975982     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.D048132     Document Type: Article

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