A sphingosine kinase activity assay using direct infusion electrospray ionization tandem mass spectrometry

Analytical Biochemistry

Volumn 380, Issue 1, 2008, Pages 35-40

  Jin, You Xun ^a   Shi, Lian Hua ^a   Yoo, Hwan Soo ^a   Lee, Yong Moon ^a   Kihara, Akio ^b   Igarashi, Yasuyuki ^b   So, Hun Young ^c   Yim, Yong Hyeon ^c  

^a Chungbuk National University   (South Korea)

^b HOKKAIDO UNIVERSITY   (Japan)

^c Korea Research Institute of Standards and Science   (South Korea)

Author keywords

Activity; Electrospray ionization; F9 12 cells; Sphingosine kinase; Sphingosine 1 phosphate; Tandem mass spectrometry

Indexed keywords

ALKANOLAMINES; ENZYMES; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; SPHINGOSINES; THERMODYNAMIC PROPERTIES;

BIOLOGICAL FUNCTIONS; CHEMICAL DERIVATIZATION; CHROMATOGRAPHIC SEPARATIONS; ELECTROSPRAY IONIZATION TANDEM MASS SPECTROMETRY; NEGATIVE ELECTROSPRAY IONIZATIONS; SPHINGOSINE; SPHINGOSINE KINASE; TANDEM MASS SPECTROMETRY;

ELECTROSPRAY IONIZATION;

PHOSPHOTRANSFERASE INHIBITOR; RADIOACTIVE MATERIAL; SPHINGOSINE 1 PHOSPHATE; SPHINGOSINE KINASE;

ANIMAL CELL; ARTICLE; CANCER CELL CULTURE; CHROMATOGRAPHY; CONTROLLED STUDY; DERIVATIZATION; ELECTROSPRAY MASS SPECTROMETRY; EMBRYO; ENZYME ACTIVITY; ENZYME ANALYSIS; MOUSE; NEGATIVE ION ELECTROSPRAY; NONHUMAN; PRIORITY JOURNAL; TERATOCARCINOMA; VALIDATION PROCESS;

MURINAE;

EID: 46649116066     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.05.021     Document Type: Article

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