Aggregate culture of human embryonic stem cell-derived hepatocytes in suspension are an improved In Vitro model for drug metabolism and toxicity testing

Toxicological Sciences

Volumn 140, Issue 1, 2014, Pages 236-245

  Sengupta, Srikumar ^a   Johnson, Brian Patrick ^b   Swanson, Scott Allen ^a   Stewart, Ron ^a   Bradfield, Christopher Alan ^b   Thomson, James Alexander ^a,c,d  

^a MORGRIDGE INSTITUTE FOR RESEARCH   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

^c UNIVERSITY OF WISCONSIN MADISON   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Drug testing; ES cells; Hepatocytes; IPS cells; Stem cells; Toxicity testing

Indexed keywords

ALBUMIN; ALPHA FETOPROTEIN; CYTOCHROME P450 1A1; CYTOCHROME P450 1A2; GLUCOSE 6 PHOSPHATASE; MESSENGER RNA; OMEPRAZOLE; OMEPRAZOLE SULFONE; PARACETAMOL; PROLINE DEHYDROGENASE; RIFAMPICIN; SERUM AND GLUCOCORTICOID REGULATED KINASE 1; CYP1A2 PROTEIN, HUMAN; CYP3A4 PROTEIN, HUMAN; CYTOCHROME P450 1A2 INDUCER; CYTOCHROME P450 3A; CYTOCHROME P450 3A INDUCER;

ADHERENT CELL; ARTICLE; CELL DIFFERENTIATION; CELL STRUCTURE; CELL SURVIVAL; DRUG HALF LIFE; DRUG METABOLISM; DRUG TRANSPORT; EMBRYO; EMBRYONIC STEM CELL; GENE EXPRESSION; GLUCURONIDATION; HEPG2 CELL LINE; HUMAN; HUMAN CELL; IN VITRO STUDY; LIQUID CHROMATOGRAPHY; LIVER CELL; METABOLITE; PRIMARY CELL; RNA ISOLATION; SUSPENSION CELL CULTURE; TANDEM MASS SPECTROMETRY; TOXICITY TESTING; UPREGULATION; BIOSYNTHESIS; CELL AGGREGATION; CULTURE TECHNIQUE; CYTOLOGY; DRUG DEVELOPMENT; DRUG EFFECTS; ENZYME INDUCTION; ENZYMOLOGY; METABOLISM; PLURIPOTENT STEM CELL; PROCEDURES; TOXICITY;

ACETAMINOPHEN; CELL AGGREGATION; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CHROMATOGRAPHY, LIQUID; CYTOCHROME P-450 CYP1A2; CYTOCHROME P-450 CYP1A2 INDUCERS; CYTOCHROME P-450 CYP3A; CYTOCHROME P-450 CYP3A INDUCERS; DRUG DISCOVERY; EMBRYONIC STEM CELLS; ENZYME INDUCTION; HEP G2 CELLS; HEPATOCYTES; HUMANS; OMEPRAZOLE; PLURIPOTENT STEM CELLS; RIFAMPIN; TANDEM MASS SPECTROMETRY; TOXICITY TESTS;

EID: 84903840046     PISSN: 10966080     EISSN: 10960929     Source Type: Journal    
DOI: 10.1093/toxsci/kfu069     Document Type: Article

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