Ionic liquid and deep eutectic solvent-activated CelA2 variants generated by directed evolution

Applied Microbiology and Biotechnology

Volumn 98, Issue 12, 2014, Pages 5775-5785

  Lehmann, Christian ^a   Bocola, Marco ^a   Streit, Wolfgang R ^b   Martinez, Ronny ^a   Schwaneberg, Ulrich ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b UNIVERSITY OF HAMBURG   (Germany)

Author keywords

Cellulase CelA2; Directed evolution; Protein engineering; Salt activation

Indexed keywords

BIOMASS; CATALYSIS; CELLULOSE; CHEMICAL ACTIVATION; CHEMICAL BONDS; CHLORINE COMPOUNDS; COMPUTER SIMULATION; DISSOLUTION; ENZYME ACTIVITY; IONIC STRENGTH;

1-BUTYL-3-METHYLIMIDAZOLIUM CHLORIDE; CELLULASE CELA2; CELLULOSE DEGRADATION; COMPUTATIONAL RESULTS; DIRECTED EVOLUTION; ENZYMATIC DEGRADATION; LIGNOCELLULOSE DEGRADATION; PROTEIN ENGINEERING;

IONIC LIQUIDS;

AMINO ACID; BETA 1,4 ENDONUCLEASE; CELLULASE; CHOLINE; ENDONUCLEASE; GLUCAN SYNTHASE; GLYCEROL; IONIC LIQUID; POTASSIUM DIHYDROGEN PHOSPHATE; SODIUM CHLORIDE; UNCLASSIFIED DRUG; CELA ENDOGLUCANASE; ENZYME ACTIVATOR; SOLVENT;

ALCOHOL; BIODEGRADATION; BIOENGINEERING; BIOMASS; CATALYSIS; CELLULOSE; CHLORIDE; ENZYME ACTIVITY; MOLECULAR ANALYSIS; PHOSPHATE; PHYSICOCHEMICAL PROPERTY; PROTEIN; SOLVENT;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVITY; ESCHERICHIA COLI; GENETIC VARIABILITY; IONIC STRENGTH; NONHUMAN; NUCLEOTIDE SEQUENCE; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; CHEMISTRY; GENETICS; METABOLISM;

CELLULASE; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVATORS; IONIC LIQUIDS; SOLVENTS;

EID: 84903814553     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-014-5771-y     Document Type: Article

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