Molecular test based on isothermal helicase-dependent amplification for detection of the Clostridium difficile toxin a gene

Journal of Clinical Microbiology

Volumn 52, Issue 7, 2014, Pages 2386-2389

  Eckert, Catherine ^a,b   Holscher, Eleonore ^a   Petit, Amandine ^a,b   Lalande, Valérie ^a,b   Barbut, Frédéric ^a,b  

^a HÔPITAL SAINT ANTOINE   (France)

^b SORBONNE UNIVERSITÉ   (France)

Author keywords

[No Author keywords available]

Indexed keywords

GLUTAMATE DEHYDROGENASE; BACTERIAL TOXIN; ENTEROTOXIN; TCDA PROTEIN, CLOSTRIDIUM DIFFICILE;

ARTICLE; BACTERIUM DETECTION; BACTERIUM EXAMINATION; BACTERIUM IDENTIFICATION; CLOSTRIDIUM DIFFICILE INFECTION; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; FECES ANALYSIS; GDH GENE; GENETIC ALGORITHM; HUMAN; ISOTHERMAL HELICASE DEPENDENT AMPLIFICATION; NONHUMAN; PREDICTIVE VALUE; PRIORITY JOURNAL; PROSPECTIVE STUDY; SENSITIVITY AND SPECIFICITY; ADOLESCENT; ADULT; AGED; CHILD; CLOSTRIDIUM INFECTIONS; COMPARATIVE STUDY; DIARRHEA; EVALUATION STUDY; FEMALE; GENETICS; ISOLATION AND PURIFICATION; MALE; MICROBIOLOGY; MIDDLE AGED; MOLECULAR DIAGNOSIS; NUCLEIC ACID AMPLIFICATION; PEPTOCLOSTRIDIUM DIFFICILE; PRESCHOOL CHILD; PROCEDURES; VERY ELDERLY; YOUNG ADULT;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; BACTERIAL TOXINS; CHILD; CHILD, PRESCHOOL; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; DIARRHEA; ENTEROTOXINS; FEMALE; HUMANS; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PREDICTIVE VALUE OF TESTS; SENSITIVITY AND SPECIFICITY; YOUNG ADULT;

EID: 84903787452     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00353-14     Document Type: Article

References (20)

1. McFarland LV, Mulligan ME, Kwok RY, Stamm WE. 1989. Nosocomial acquisition of Clostridium difficile infection. N. Engl. J. Med. 320:204-210. http://dx.doi.org/10.1056/NEJM198901263200402.
2. Planche T, Wilcox M. 2011. Reference assays for Clostridium difficile infection: one or two gold standards? J. Clin. Pathol. 64:1-5. http://dx.doi.org/10.1136/jcp.2010.080135.
3. Crobach MJ, Dekkers OM, Wilcox MH, Kuijper EJ. 2009. European Society of Clinical Microbiology and Infectious Diseases (ESCMID): data review and recommendations for diagnosing Clostridium difficileinfection (CDI). Clin. Microbiol. Infect. 15:1053-1066. http://dx.doi.org/10.1111/j.1469-0691.2009. 03098.x.
4. René P, Frenette CP, Schiller I, Dendukuri N, Brassard P, Fenn S, Loo VG. 2012. Comparison of eight commercial enzyme immunoassays for the detection of Clostridium difficile from stool samples and effect of strain type. Diagn. Microbiol. Infect. Dis. 73:94-96. http://dx.doi.org/10.1016/j. diagmicrobio.2012.01.005.
5. Cohen SH, Gerding DN, Johnson S, Kelly CP, Loo VG, McDonald LC, Pepin J, Wilcox MH. 2010. Clinical practice guidelines for Clostridium difficile infection in adults: 2010 update by the Society for Healthcare Epidemiology of America (SHEA) and the Infectious Diseases Society of America (IDSA). Infect. Control. Hosp. Epidemiol. 31:431-455. http://dx.doi.org/10.1086/651706.
6. American Society for Microbiology. 2010. A practical guidance document for the laboratory detection of toxigenic Clostridium difficile. American Society for Microbiology, Washington, DC.
7. Shetty N, Wren MWD, Coen PG. 2011. The role of glutamate dehydrogenase for the detection of Clostridium difficile in faecal samples: a metaanalysis. J. Hosp. Infect. 77:1-6. http://dx.doi.org/10.1016/j.jhin.2010.07.024.
8. O'Horo JC, Jones A, Sternke M, Harper C, Safdar N. 2012. Molecular techniques for diagnosis of Clostridium difficile infection: systematic review and meta-analysis. Mayo Clin. Proc. 87:643-651. http://dx.doi.org/10.1016/j. mayocp.2012.02.024.
9. Chang TW, Gorbach SL, Bartlett JB. 1978. Neutralization of Clostridium difficile toxin by Clostridium sordellii antitoxins. Infect. Immun. 22:418-422.
10. Barbut F, Gariazzo B, Bonne L, Lalande V, Burghoffer B, Luiuz R, Petit JC. 2007. Clinical features of Clostridium difficile-associated infections and molecular characterization of strains: results of a retrospective study, 2000-2004. Infect. Control. Hosp. Epidemiol. 28:131-139. http://dx.doi.org/10. 1086/511794.
11. Eckert C, Coignard B, Hebert M, Tarnaud C, Tessier C, Lemire A, Burghoffer B, Noel D, Barbut F, ICD-Raisin Working Group. 2013. Clinical and microbiological features of Clostridium difficile infections in France: the ICD-RAISIN 2009 national survey. Med. Mal. Infect. 43:67-74. http://dx.doi.org/10.1016/j.medmal.2013.01.004.
12. Barbut F, Lalande V, Eckert C. 2012. New molecular methods for the diagnosis of Clostridium difficile infections. Drugs Today (Barc.) 48:673-684.
13. Brecher SM, Novak-Weekley SM, Nagy E. 2013. Laboratory diagnosis of Clostridium difficile infections: there is light at the end of the colon. Clin. Infect. Dis. 57:1175-1181. http://dx.doi.org/10.1093/cid/cit424.
14. Deak E, Miller SA, Humphries RM. 2014. Comparison of Illumigene, Simplexa, and AmpliVue Clostridium difficile molecular assays for diagnosis of C. difficile infection. J. Clin. Microbiol. 52:960-963. http://dx.doi.org/10. 1128/JCM.02354-13.
15. Lalande V, Barrault L, Wadel S, Eckert C, Petit JC, Barbut F. 2011. Evaluation of a loop-mediated isothermal amplification assay for diagnosis of Clostridium difficile infections. J. Clin. Microbiol. 49:2714-2716. http://dx.doi.org/10.1128/JCM.01835-10.
16. Bamber AI, Fitzsimmons K, Cunniffe JG, Beasor CC, Mackintosh CA, Hobbs G. 2012. Diagnosis of Clostridium difficile-associated disease: examination of multiple algorithms using toxin EIA, glutamate dehydrogenase EIA and loop-mediated isothermal amplification. Br. J. Biomed. Sci. 69:112-118.
17. Walkty A, Lagacé-Wiens PRS, Manickam K, Adam H, Pieroni P, Hoban D, Karlowsky JA, Alfa M. 2013. Evaluation of an algorithmic approach in comparison with the Illumigene assay for laboratory diagnosis of Clostridium difficile infection. J. Clin. Microbiol. 51:1152-1157. http://dx.doi.org/10. 1128/JCM.03203-12.
18. Squire MM, Carter GP, Mackin KE, Chakravorty A, Norén T, Elliott B, Lyras D, Riley TV. 2013. Novel molecular type of Clostridium difficile in neonatal pigs, western Australia. Emerg. Infect. Dis. 19:790-792. http://dx.doi.org/10.3201/eid1905.121062.
19. Geric Stare B, Rupnik M. 2010. Clostridium difficile toxinotype XI (A-B-) exhibits unique arrangement of PaLoc and its upstream region. Anaerobe 16:393-395. http://dx.doi.org/10.1016/j.anaerobe.2010.04.001.
20. Planche TD, Davies KA, Coen PG, Finney JM, Monahan IM, Morris KA, O'Connor L, Oakley SJ, Pope CF, Wren MW, Shetty NP, Crook DW, Wilcox MH. 2013. Differences in outcome according to Clostridium difficile testing method: a prospective multicentre diagnostic validation study of C difficile infection. Lancet Infect. Dis. 13:936-945. http://dx.doi.org/10.1016/S1473-3099(13)70200-7.