Comparison of eight commercial enzyme immunoassays for the detection of Clostridium difficile from stool samples and effect of strain type

Diagnostic Microbiology and Infectious Disease

Volumn 73, Issue 1, 2012, Pages 94-96

  René, Pierre ^a   Frenette, Charles P ^a   Schiller, Ian ^a   Dendukuri, Nandini ^a   Brassard, Paul ^a   Fenn, Susan ^a   Loo, Vivian G ^a  

^a MCGILL UNIVERSITY HEALTH CENTRE   (Canada)

Author keywords

Clostridium difficile; Diagnostic assays

Indexed keywords

BACTERIAL ANTIGEN; BACTERIAL TOXIN; NUCLEOSOME ASSEMBLY PROTEIN 1;

ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATION; CELL ASSAY; CELL CYTOTOXICITY NEUTRALIZATION ASSAY; CLOSTRIDIUM DIFFICILE; CONTROLLED STUDY; ENZYME IMMUNOASSAY; FECES ANALYSIS; GENOTYPE; INTERMETHOD COMPARISON; NONHUMAN; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; SENSITIVITY AND SPECIFICITY;

BACTERIAL TOXINS; BACTERIOLOGICAL TECHNIQUES; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; FECES; HUMANS; IMMUNOENZYME TECHNIQUES; SENSITIVITY AND SPECIFICITY;

EID: 84860766309     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2012.01.005     Document Type: Article

References (19)

1. Barbut F., Corthier G., Charpak Y., Cerf M., Monteil H., Fosse T., et al. Prevalence and pathogenicity of Clostridium difficile in hospitalized patients. Arch. Intern. Med. 1996, 156:1449-1454.
2. Eastwood K., Else P., Charlett A., Wilcox M. Comparison of nine commercially available Clostridium difficile toxin detection assays, a real-time PCR assay for C. difficile tcdB, and a glutamate dehydrogenase detection assay to cytotoxin testing and cytotoxigenic culture methods. J. Clin. Microbiol. 2009, 47:3211-3217.
3. Fawley W.N., Wilcox M.H. Pulsed-field gel electrophoresis can yield DNA fingerprints of degradation-susceptible Clostridium difficile strains. J. Clin. Microbiol. 2002, 40:3546-3547.
4. Gilligan P.H. Is a two-step glutamate dehydrogenase antigen-cytotoxicity neutralization assay algorithm superior to the premier toxin A and B enzyme immunoassay for laboratory detection of Clostridium difficile?. J. Clin. Microbiol. 2008, 46:1523-1525.
5. Kvach E., Ferguson D., Riska P.F., Landry M.L. Comparison of BD GeneOhm Cdiff Real-Time PCR assay with a two-step algorithm and a Toxin A/B enzyme-linked immunosorbent assay for diagnosis of toxigenic Clostridium difficile infection. J. Clin. Microbiol. 2010, 48:109-114.
6. McDonald L.C. Clostridium difficile: responding to a new threat from an old enemy. Infect. Control Hosp. Epidemiol. 2005, 26:672-675.
7. Miller M.A., Gravel D., Mulvey M., Taylor G., Boyd D., Simor A., et al. Health care-associated Clostridium difficile infection in Canada: patient age and infecting strain type are highly predictive of severe outcome and mortality. Clin. Infect. Dis. 2010, 50:194-201.
8. Novak-Weekley S., Marlowe E., Miller J., Cumpio J., Nomura J., Vance P., et al. Clostridium difficile testing in the clinical laboratory by use of multiple testing algorithms. J Clin. Microbiol. 2010, 48:889-893.
9. Peterson L.R., Kelly P.J. The role of the clinical microbiology laboratory in the management of Clostridium difficile-associated diarrhea. Infect. Dis. Clin. North Am. 1993, 7:277-293.
10. Quinn C.D., Sefers S.E., Babike W., He Y., Alcabasa R., Stratton C.W., et al. C. Diff Quik Chek Complete enzyme immunoassay provides a reliable first-line method for detection of Clostridium difficile in stool specimens. J. Clin. Microb. 2010, 48:603-605.
11. Reller M.E., Alcabasa R.C., Lema C.A., Carroll K.C. Comparison of two rapid assays for Clostridium difficile common antigen and a C difficile toxin A/B assay with the cell neutralization assay. Am. J. Clin. Pathol. 2010, 133:107-109.
12. Reller M., Lema C., Perl T., Cai M., Ross T., Speck K., et al. Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for detection of toxigenic Clostridium difficile. J. Clin. Microbiol. 2007, 45:3601-3605.
13. Sharp S.E., Ruden L.O., Pohl J.C., Hatcher P.A., Jayne L.M., Ivie W.M. Evaluation of the C. Diff Quik Chek complete assay, a new glutamate dehydrogenase and A/B toxin combination lateral flow assay for use in the rapid, simple diagnosis of Clostridium difficile disease. J. Clin. Microbiol. 2010, 48:2082-2086.
14. Swindells J., Brenwald N., Reading N., Oppenheim B. Evaluation of diagnostic tests for Clostridium difficile infection. J. Clin. Microbiol. 2010, 48:606-608.
15. Tenover F.C., Arbeit R.D., Goering R.V., Mickelsen P.A., Murray B.E., Persing D.H., et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 1995, 33:2233-2239.
16. Tenover F.C., Novak-Weekley S., Woods C.W., Peterson L.R., Davis T., Schreckenberger P., et al. Impact of strain type on detection of toxigenic Clostridium difficile: comparison of molecular diagnostic and enzyme immunoassay approaches. J. Clin. Microbiol. 2010, 48:3719-3724.
17. Ticehurst J., Aird D., Dam L., Borek A., Hargrove J., Carroll K. Effective detection of toxigenic Clostridium difficile by a two-step algorithm including tests for antigen and cytotoxin. J. Clin. Microbiol. 2006, 44:1145-1149.
18. Turgeon D.K., Novick T.J., Quick J., Carlson L., Miller P. Six rapid tests for direct detection of Clostridium difficile and its toxins in fecal samples compared with the fibroblast cytotoxicity assay. J. Clin. Microbiol. 2003, 41:667-670.
19. Warny M., Pepin J., Fang A., Killgore G., Thompson A., Brazie J., et al. Toxin production by an emerging strain of Clostridium difficile associated with outbreaks of severe disease in North America and Europe. Lancet 2005, 366:1079-1084.