Small scale affinity purification and high sensitivity reversed phase nanoLC-MS N-glycan characterization of mAbs and fusion proteins

mAbs

Volumn 6, Issue 4, 2014, Pages 894-903

  Higel, Fabian ^a   Seidl, Andreas ^a   Demelbauer, Uwe ^a   Sor̈gel, Fritz ^b,c   Frieß, Wolfgang ^d  

^a Sandoz Biopharmaceuticals   (Germany)

^b Institute for Biomedical Research   (Germany)

^c UNIVERSITY OF ESSEN MEDICAL SCHOOL   (Germany)

^d UNIVERSITY OF MUNICH   (Germany)

Author keywords

Biomarker discovery; Fusion protein; Mass spectrometry; N glycosylation; NanoLC; Oligosaccharide; Therapeutic antibody

Indexed keywords

ANTHRANILIC ACID; HYBRID PROTEIN; IMMUNOGLOBULIN G; PROTEIN A; PROTEIN G; ANTHRANILIC ACID DERIVATIVE; MONOCLONAL ANTIBODY; POLYSACCHARIDE;

ARTICLE; CONTROLLED STUDY; DOWNSTREAM PROCESSING; ELECTROSPRAY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; LIMIT OF QUANTITATION; MASS SPECTROMETRY; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; REVERSED PHASE LIQUID CHROMATOGRAPHY; REVERSED PHASE NANO LIQUID CHORMATOGRAPHY MASS SPECTROMETRY; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; AFFINITY CHROMATOGRAPHY; ANIMAL; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; PROCEDURES;

ANIMALS; ANTIBODIES, MONOCLONAL; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, REVERSE-PHASE; HUMANS; ORTHO-AMINOBENZOATES; POLYSACCHARIDES; RECOMBINANT FUSION PROTEINS;

EID: 84903763232     PISSN: 19420862     EISSN: 19420870     Source Type: Journal    
DOI: 10.4161/mabs.29263     Document Type: Article

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