Nanoimaging of focal adhesion dynamics in 3D

PLoS ONE

Volumn 9, Issue 6, 2014, Pages

  Chiu, Chi Li ^a   Aguilar, Jose S ^a   Tsai, Connie Y ^b   Wu, GuiKai ^b   Gratton, Enrico ^a   Digman, Michelle A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; ALPHA5 INTEGRIN; COLLAGEN; COLLAGEN FIBER; ENHANCED GREEN FLUORESCENT PROTEIN; PAXILLIN; COLLAGEN TYPE 1; PROTEIN BINDING;

ANALYTICAL PARAMETERS; ARTICLE; CELL FUNCTION; CELL GROWTH; CELL LINE; CELL MIGRATION; CELL STRUCTURE; DIFFUSION; FLUORESCENCE CORRELATION SPECTROSCOPY; FOCAL ADHESION; IMAGING AND DISPLAY; MDA MB 231 CELL; NANOIMAGING; NUMBER AND MOLECULAR BRIGHTNESS; PROTEIN AGGREGATION; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN LOCALIZATION; PROTEIN POLYMERIZATION; PROTEIN TRANSPORT; RAPID BEAM OSCILLATION TECHNIQUE; U2OS CELL LINE; CELL CULTURE TECHNIQUE; CHEMISTRY; HUMAN; METABOLISM; NANOTECHNOLOGY; PROCEDURES; PROTEIN CONFORMATION; SPECTROFLUOROMETRY; ULTRASTRUCTURE;

ACTINS; CELL CULTURE TECHNIQUES; CELL LINE; COLLAGEN TYPE I; FOCAL ADHESIONS; HUMANS; NANOTECHNOLOGY; PAXILLIN; PROTEIN BINDING; PROTEIN CONFORMATION; SPECTROMETRY, FLUORESCENCE;

EID: 84903533368     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0099896     Document Type: Article

References (39)

1. Kuo JC, Han X, Hsiao CT, Yates Iii JR, Waterman CM (2011) Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for beta-Pix in negative regulation of focal adhesion maturation. Nat Cell Biol 13: 383-393.
2. Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, Davidson MW, et al. (2010) Nanoscale architecture of integrin-based cell adhesions. Nature 468: 580-584.
3. Gardel ML, Schneider IC, Aratyn-Schaus Y, Waterman CM (2010) Mechanical integration of actin and adhesion dynamics in cell migration. Annu Rev Cell Dev Biol 26: 315-333.
4. Geiger B, Spatz JP, Bershadsky AD (2009) Environmental sensing through focal adhesions. Nature Reviews Molecular Cell Biology 10: 21-33.
5. Geiger B, Bershadsky A (2001) Assembly and mechanosensory function of focal contacts. Current Opinion in Cell Biology 13: 584-592. (Pubitemid 32817017)
6. Hayakawa K, Tatsumi H, Sokabe M (2008) Actin stress fibers transmit and focus force to activate mechanosensitive channels. Journal of Cell Science 121: 496-503. (Pubitemid 351405058)
7. Parsons JT, Horwitz AR, Schwartz MA (2010) Cell adhesion: integrating cytoskeletal dynamics and cellular tension. Nat Rev Mol Cell Biol 11: 633-643.
8. Webb DJ, Parsons JT, Horwitz AF (2002) Adhesion assembly, disassembly and turnover in migrating cells - over and over and over again. Nature Cell Biology 4: E97-E100. (Pubitemid 34308850)
9. Harunaga JS, Yamada KM (2011) Cell-matrix adhesions in 3D. Matrix Biol.
10. Kubow KE, Horwitz AR (2011) Reducing background fluorescence reveals adhesions in 3D matrices. Nat Cell Biol 13: 3-5; author reply 5-7.
11. Fraley SI, Feng YF, Wirtz D, Longmore GD (2011) Reply: reducing background fluorescence reveals adhesions in 3D matrices. Nature Cell Biology 13: 5-U254.
12. Fraley SI, Feng Y, Krishnamurthy R, Kim DH, Celedon A, et al. (2010) A distinctive role for focal adhesion proteins in three-dimensional cell motility. Nat Cell Biol 12: 598-604.
13. Meyer AS, Hughes-Alford SK, Kay JE, Castillo A, Wells A, et al. (2012) 2D protrusion but not motility predicts growth factor-induced cancer cell migration in 3D collagen. Journal of Cell Biology 197: 721-729.
14. Kraning-Rush CM, Carey SP, Califano JP, Smith BN, Reinhart-King CA (2011) The role of the cytoskeleton in cellular force generation in 2D and 3D environments. Phys Biol 8: 015009.
15. Yu XZ, Machesky LM (2012) Cells Assemble Invadopodia-Like Structures and Invade into Matrigel in a Matrix Metalloprotease Dependent Manner in the Circular Invasion Assay. PLoS One 7.
16. Deakin NO, Turner CE (2011) Distinct roles for paxillin and Hic-5 in regulating breast cancer cell morphology, invasion, and metastasis. Molecular Biology of the Cell 22: 327-341.
17. Rossow MJ, Sasaki JM, Digman MA, Gratton E (2010) Raster image correlation spectroscopy in live cells. Nat Protoc 5: 1761-1774.
18. Digman MA, Wiseman PW, Choi C, Horwitz AR, Gratton E (2009) Stoichiometry of molecular complexes at adhesions in living cells. Proceedings of the National Academy of Sciences of the United States of America 106: 2170-2175.
19. Schaller MD (2001) Paxillin: a focal adhesion-associated adaptor protein. Oncogene 20: 6459-6472.
20. Turner CE, Deakin NO (2008) Paxillin comes of age. Journal of Cell Science 121: 2435-2444.
21. Digman MA, Brown CM, Horwitz AR, Mantulin WW, Gratton E (2008) Paxillin dynamics measured during adhesion assembly and disassembly by correlation spectroscopy. Biophysical Journal 94: 2819-2831.
22. Lanzano L, Digman MA, Fwu P, Giral H, Levi M, et al. (2011) Nanometer-scale imaging by the modulation tracking method. Journal of Biophotonics 4: 415-424.
23. Levi V, Ruan QQ, Gratton E (2005) 3-D particle tracking in a two-photon microscope: Application to the study of molecular dynamics in cells. Biophysical Journal 88: 2919-2928. (Pubitemid 40976156)
24. Kis-Petikova K, Gratton E (2004) Distance measurement by circular scanning of the excitation beam in the two-photon microscope. Microscopy Research and Technique 63: 34-49. (Pubitemid 37541969)
25. Rossow M, Mantulin WW, Gratton E (2009) Spatiotemporal image correlation spectroscopy measurements of flow demonstrated in microfluidic channels. Journal of Biomedical Optics 14.
26. Hebert B, Costantino S, Wiseman PW (2005) Spatiotemporal image correlation spectroscopy (STICS) theory, verification, and application to protein velocity mapping in living CHO cells. Biophysical Journal 88: 3601-3614. (Pubitemid 40586608)
27. Tanner K, Ferris DR, Lanzano L, Mandefro B, Mantulin WW, et al. (2009) Coherent Movement of Cell Layers during Wound Healing by Image Correlation Spectroscopy. Biophysical Journal 97: 2098-2106.
28. Digman MA, Brown CM, Sengupta P, Wiseman PW, Horwitz AR, et al. (2005) Measuring fast dynamics in solutions and cells with a laser scanning microscope. Biophysical Journal 89: 1317-1327. (Pubitemid 41099013)
29. Digman MA, Dalal R, Horwitz AF, Gratton E (2008) Mapping the number of molecules and brightness in the laser scanning microscope. Biophysical Journal 94: 2320-2332.
30. Wolf K, Mazo I, Leung H, Engelke K, von Andrian UH, et al. (2003) Compensation mechanism in tumor cell migration: mesenchymal-amoeboid transition after blocking of pericellular proteolysis. Journal of Cell Biology 160: 267-277. (Pubitemid 36254960)
31. McGrath JL, Tardy Y, Dewey CF, Meister JJ, Hartwig JH (1998) Simultaneous measurements of actin filament turnover, filament fraction, and monomer diffusion in endothelial cells. Biophysical Journal 75: 2070-2078. (Pubitemid 28455203)
32. Wolf K, Alexander S, Schacht V, Coussens LM, von Andrian UH, et al. (2009) Collagen-based cell migration models in vitro and in vivo. Seminars in Cell & Developmental Biology 20: 931-941.
33. Maskarinec SA, Franck C, Tirrell DA, Ravichandran G (2009) Quantifying cellular traction forces in three dimensions. Proc Natl Acad Sci U S A 106: 22108-22113.
34. Turner CE, Deakin NO (2011) Distinct roles for paxillin and Hic-5 in regulating breast cancer cell morphology, invasion, and metastasis. Molecular Biology of the Cell 22: 327-341.
35. Rhee S (2009) Fibroblasts in three dimensional matrices: cell migration and matrix remodeling. Experimental and Molecular Medicine 41: 858-865.
36. Pathak A, Kumar S (2011) Biophysical regulation of tumor cell invasion: moving beyond matrix stiffness. Integr Biol (Camb).
37. Grinnell F, Miron-Mendoza M, Seemann J (2010) The differential regulation of cell motile activity through matrix stiffness and porosity in three dimensional collagen matrices. Biomaterials 31: 6425-6435.
38. Friedl P, Wolf K (2010) Plasticity of cell migration: a multiscale tuning model. J Cell Biol 188: 11-19.
39. Poincloux R, Collin O, Lizarraga F, Romao M, Debray M, et al. (2011) Contractility of the cell rear drives invasion of breast tumor cells in 3D Matrigel. Proc Natl Acad Sci U S A 108: 1943-1948.