A spectrophotometer-based diffusivity assay reveals that diffusion hindrance of small molecules in extracellular matrix gels used in 3D cultures is dominated by viscous effects

Colloids and Surfaces B: Biointerfaces

Volumn 120, Issue , 2014, Pages 200-207

  Galgoczy, Roland ^a   Pastor, Isabel ^a   Colom, Adai ^a,c   Giménez, Alicia ^a   Mas, Francesc ^a   Alcaraz, Jordi ^a,b  

^a UNIVERSITY OF BARCELONA   (Spain)

^b INSTITUTO DE SALUD CARLOS III   (Spain)

^c Université Aix Marseille   (France)

Author keywords

3D culture; Collagen; Diffusion; Fibrin; Matrigel; Stokes Einstein equation

Indexed keywords

ASSAYS; COLLAGEN; CYTOLOGY; GELS; MACROMOLECULES; METEOROLOGICAL INSTRUMENTS; PHYSIOLOGY; SPECTROPHOTOMETERS; TISSUE; VISCOSITY;

3-D CULTURES; APPARENT DIFFUSIVITY; EXTRACELLULAR MATRICES; FIBRIN; MACROMOLECULAR TRANSPORT; MATRIGEL; PHYSIOLOGICAL RANGE; STOKES EINSTEIN EQUATIONS;

DIFFUSION;

COLLAGEN TYPE 1; FIBRIN; FLUORESCEIN ISOTHIOCYANATE DEXTRAN; MATRIGEL; DEXTRAN; FLUORESCEIN ISOTHIOCYANATE; GEL;

ARTICLE; ASSAY; BRAIN TISSUE; CONTROLLED STUDY; CULTURE TECHNIQUE; CYTOLOGY; DIFFUSION; DIFFUSIVITY ASSAY; EXTRACELLULAR MATRIX; FILTERS AND MEMBRANES; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; GEL; MACROMOLECULE; MATHEMATICAL PARAMETERS; MICROPLATE READER; MOLECULAR WEIGHT; PRIORITY JOURNAL; SPECTROPHOTOMETER; STOKES EINSTEIN EQUATION; THREE DIMENSIONAL CULTURE; VISCOSITY; ANALOGS AND DERIVATIVES; ATOMIC FORCE MICROSCOPY; CHEMISTRY; PROCEDURES; REPRODUCIBILITY; SPECTROPHOTOMETRY; TIME;

CELL CULTURE TECHNIQUES; DEXTRANS; DIFFUSION; EXTRACELLULAR MATRIX; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; GELS; MICROSCOPY, ATOMIC FORCE; REPRODUCIBILITY OF RESULTS; SPECTROPHOTOMETRY; TIME FACTORS; VISCOSITY;

EID: 84903513824     PISSN: 09277765     EISSN: 18734367     Source Type: Journal    
DOI: 10.1016/j.colsurfb.2014.05.017     Document Type: Article

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