Metabolic engineering of Pichia pastoris for production of hyaluronic acid with high molecular weight

Journal of Biotechnology

Volumn 185, Issue , 2014, Pages 28-36

  Jeong, Euijoon ^a   Shim, Woo Yong ^a   Kim, Jung Hoe ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Hyaluronan synthase 2; Hyaluronic acid; Metabolic engineering; Molecular weight; Pichia pastoris; Xenopus laevis

Indexed keywords

CELL GROWTH; GENE EXPRESSION; GLUCOSE; GROWTH KINETICS; METABOLIC ENGINEERING; MOLECULAR WEIGHT; ORGANIC ACIDS; POLYMERS; YEAST;

HETEROLOGOUS PROTEINS; MICROBIAL FERMENTATION; PHOSPHOGLUCOSE ISOMERASE; PICHIA PASTORIS; SYNTHASES; UDP-GLUCOSE DEHYDROGENASE; UDP-N-ACETYLGLUCOSAMINE PYROPHOSPHORYLASE; XENOPUS LAEVIS;

HYALURONIC ACID;

ALCOHOL OXIDASE; ALCOHOL OXIDASE 1; ALCOHOL OXIDASE 2; GLUCOSE 6 PHOSPHATE ISOMERASE; HYALURONAN SYNTHASE 2; HYALURONIC ACID; NUCLEOTIDYLTRANSFERASE; SYNTHETASE; UDP N ACETYLGLUCOSAMINE PYROPHOSPHORYLASE; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE GLUCOSE DEHYDROGENASE; URIDINE DIPHOSPHATE GLUCURONIC ACID; URIDINE DIPHOSPHATE N ACETYLGLUCOSAMINE; URIDINE TRIPHOSPHATE GLUCOSE 1 PHOSPHATE URIDYLYLTRANSFERASE; GLUCOSE DEHYDROGENASE; GLUCURONOSYLTRANSFERASE; HAS2 PROTEIN, XENOPUS; PRIMER DNA; XENOPUS PROTEIN;

ARTICLE; BIOSYNTHESIS; CELL GROWTH; CODON USAGE; EXPRESSION VECTOR; FERMENTATION; GEL PERMEATION CHROMATOGRAPHY; GROWTH INHIBITION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LOW TEMPERATURE; METABOLIC ENGINEERING; MOLECULAR WEIGHT; NONHUMAN; OPERON; PICHIA PASTORIS; POLYMERIZATION; PRIORITY JOURNAL; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ULTRAVIOLET DETECTOR; XENOPUS LAEVIS; ANIMAL; CHEMISTRY; DNA SEQUENCE; ESCHERICHIA COLI; GENE VECTOR; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PICHIA; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; TEMPERATURE;

ANIMALS; BASE SEQUENCE; BIOSYNTHETIC PATHWAYS; DNA PRIMERS; ESCHERICHIA COLI; GENETIC VECTORS; GLUCOSE 1-DEHYDROGENASE; GLUCURONOSYLTRANSFERASE; HYALURONIC ACID; METABOLIC ENGINEERING; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PICHIA; PROMOTER REGIONS, GENETIC; REAL-TIME POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; TEMPERATURE; XENOPUS LAEVIS; XENOPUS PROTEINS;

EID: 84903474498     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.05.018     Document Type: Article

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