Identification of promiscuous ene-reductase activity by mining structural databases using active site constellations

Nature Communications

Volumn 5, Issue , 2014, Pages

  Steinkellner, Georg ^a   Gruber, Christian C ^a   Pavkov Keller, Tea ^a   Binter, Alexandra ^a   Steiner, Kerstin ^a   Winkler, Christoph ^b   Łyskowski, Andrze ^a   Schwamberger, Orsolya ^a,b   Oberer, Monika ^b   Schwab, Helmut ^a,c   Faber, Kurt ^a,b   MacHeroux, Peter ^a,d   Gruber, Karl ^a,b  

^a AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

^b UNIVERSITY OF GRAZ   (Austria)

^c GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^d GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

1,4 BENZOQUINONE; 2 CYCLOHEXENONE; ENE REDUCTASE; OXIDOREDUCTASE; PROTON; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE DEHYDROGENASE; TYROSINE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN;

BIOCHEMISTRY; BIOENGINEERING; BIOINFORMATICS; CATALYST; CHEMICAL BINDING; CRYSTAL STRUCTURE; DATABASE; ENZYME ACTIVITY; LIGAND; PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BIOCATALYST; CRYSTAL STRUCTURE; CRYSTALLIZATION; DISSOCIATION CONSTANT; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME METABOLISM; ENZYME STRUCTURE; HYDROGEN BOND; LIGAND BINDING; NONHUMAN; OXIDATION; OXIDATION REDUCTION POTENTIAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; SACCHAROMYCES PASTORIANUS; X RAY CRYSTALLOGRAPHY; CATALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME ACTIVE SITE; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; PROTEIN CONFORMATION; PROTEIN DATABASE; THERMUS THERMOPHILUS;

BACTERIAL PROTEINS; CATALYSIS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; DATABASES, PROTEIN; KINETICS; MODELS, MOLECULAR; OXIDOREDUCTASES; PROTEIN CONFORMATION; THERMUS THERMOPHILUS;

EID: 84903189982     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms5150     Document Type: Article

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