Paracellular transport through healthy and cystic fibrosis bronchial epithelial cell lines - Do we have a proper model?

PLoS ONE

Volumn 9, Issue 6, 2014, Pages

  Molenda, Natalia ^a   Urbanova, Katarina ^a   Weiser, Nelly ^a   Kusche Vihrog, Kristina ^a   Gun̈zel, Dorothee ^b   Schillers, Hermann ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CLAUDIN 3; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; TIGHT JUNCTION PROTEIN; CFTR PROTEIN, HUMAN; CLDN3 PROTEIN, HUMAN; FLUORESCEIN;

ARTICLE; BRONCHUS MUCOSA; CELL CONTACT; CELL LINE; CELL MEMBRANE CONDUCTANCE; CELL MEMBRANE PERMEABILITY; CELL TRANSPORT; CONTROLLED STUDY; CYSTIC FIBROSIS; ELECTRIC RESISTANCE; EPITHELIUM CELL; HUMAN; HUMAN CELL; PARACELLULAR FLUORESCEIN FLUX; PROTEIN EXPRESSION; TRANSEPITHELIAL ELECTRICAL RESISTANCE; TRANSPORT KINETICS; BIOLOGICAL MODEL; BRONCHUS; GENE EXPRESSION; GENETICS; IMPEDANCE; METABOLISM; MUTATION; PATHOLOGY; PERMEABILITY; RESPIRATORY TRACT MUCOSA; SIGNAL TRANSDUCTION; TIGHT JUNCTION; TRANSPORT AT THE CELLULAR LEVEL;

BIOLOGICAL TRANSPORT; BRONCHI; CELL LINE; CLAUDIN-3; CYSTIC FIBROSIS; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; ELECTRIC IMPEDANCE; EPITHELIAL CELLS; FLUORESCEIN; GENE EXPRESSION; HUMANS; MODELS, BIOLOGICAL; MUTATION; PERMEABILITY; RESPIRATORY MUCOSA; SIGNAL TRANSDUCTION; TIGHT JUNCTIONS;

EID: 84903153642     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0100621     Document Type: Article

References (37)

1. Kapus A, Szaszi K (2006) Coupling between apical and paracellular transport processes. Biochem Cell Biol 84: 870-880.
2. Flynn AN, Itani OA, Moninger TO, Welsh MJ (2009) Acute regulation of tight junction ion selectivity in human airway epithelia. Proc Natl Acad Sci U S A 106: 3591-3596.
3. Sheppard DN, Welsh MJ (1999) Structure and function of the CFTR chloride channel. Physiol Rev 79: S23-S45.
4. Pilewski JM, Frizzell RA (1999) Role of CFTR in airway disease. Physiol Rev 79: S215-S255.
5. Welsh MJ, Smith AE (1993) Molecular mechanisms of CFTR chloride channel dysfunction in cystic fibrosis. Cell 73: 1251-1254. 0092-8674(93)90353-R [pii].
6. Guggino WB, Stanton BA (2006) New insights into cystic fibrosis: molecular switches that regulate CFTR. Nat Rev Mol Cell Biol 7: 426-436.
7. Kunzelmann K, Kiser GL, Schreiber R, Riordan JR (1997) Inhibition of epithelial Na + currents by intracellular domains of the cystic fibrosis transmembrane conductance regulator. FEBS Lett 400: 341-344.
8. Treharne KJ, Xu Z, Chen JH, Best OG, Cassidy DM, et al. (2009) Inhibition of protein kinase CK2 closes the CFTR Cl channel, but has no effect on the cystic fibrosis mutant deltaF508-CFTR. Cell Physiol Biochem 24: 347-360. 000257427 [pii];10.1159/000257427 [doi].
9. Bobadilla JL, Macek M Jr, Fine JP, Farrell PM (2002) Cystic fibrosis: a worldwide analysis of CFTR mutations-correlation with incidence data and application to screening. Hum Mutat 19: 575-606.
10. Jiang C, Finkbeiner WE, Widdicombe JH, McCray PB Jr, Miller SS (1993) Altered fluid transport across airway epithelium in cystic fibrosis. Science 262: 424-427.
11. Jiang C, Finkbeiner WE, Widdicombe JH, Miller SS (1997) Fluid transport across cultures of human tracheal glands is altered in cystic fibrosis. J Physiol 501 (Pt 3): 637-647.
12. Weiser N, Molenda N, Urbanova K, Bahler M, Pieper U, et al. (2011) Paracellular Permeability of Bronchial Epithelium is Controlled by CFTR. Cell Physiol Biochem 28: 289-296. 000331742 [pii];10.1159/000331742 [doi].
13. Powell DW (1981) Barrier function of epithelia. Am J Physiol 241: G275-G288.
14. Lewis SA, Berg JR, Kleine TJ (1995) Modulation of epithelial permeability by extracellular macromolecules. Physiol Rev 75: 561-589.
15. Turner JR (2006) Molecular basis of epithelial barrier regulation: from basic mechanisms to clinical application. Am J Pathol 169: 1901-1909.
16. Anderson JM, Van Itallie CM (2009) Physiology and function of the tight junction. Cold Spring Harb Perspect Biol 1: a002584. 10.1101/cshperspect.a002584 [doi].
17. Shen L, Weber CR, Raleigh DR, Yu D, Turner JR (2011) Tight junction pore and leak pathways: a dynamic duo. Annu Rev Physiol 73: 283-309. 10.1146/annurev-physiol-012110-142150 [doi].
18. Illek B, Maurisse R, Wahler L, Kunzelmann K, Fischer H, et al. (2008) Cl transport in complemented CF bronchial epithelial cells correlates with CFTR mRNA expression levels. Cell Physiol Biochem 22: 57-68.
19. Jou TS, Schneeberger EE, Nelson WJ (1998) Structural and functional regulation of tight junctions by RhoA and Rac1 small GTPases. J Cell Biol 142: 101-115.
20. Cozens AL, Yezzi MJ, Kunzelmann K, Ohrui T, Chin L, et al. (1994) CFTR expression and chloride secretion in polarized immortal human bronchial epithelial cells. Am J Respir Cell Mol Biol 10: 38-47.
21. Ehrhardt C, Kneuer C, Fiegel J, Hanes J, Schaefer UF, et al. (2002) Influence of apical fluid volume on the development of functional intercellular junctions in the human epithelial cell line 16HBE14o-: implications for the use of this cell line as an in vitro model for bronchial drug absorption studies. Cell Tissue Res 308: 391-400.
22. Wan H, Winton HL, Soeller C, Stewart GA, Thompson PJ, et al C (2000) Tight junction properties of the immortalized human bronchial epithelial cell lines Calu-3 and 16HBE14o-. Eur Respir J 15: 1058-1068.
23. Ehrhardt C, Collnot EM, Baldes C, Becker U, Laue M, et al. (2006) Towards an in vitro model of cystic fibrosis small airway epithelium: characterisation of the human bronchial epithelial cell line CFBE41o-. Cell Tissue Res 323: 405-415.
24. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25: 402-408. 10.1006/meth.2001.1262 [doi];S1046-2023(01)91262-9 [pii].
25. Gonzalez-Mariscal L, Chavez de RB, Lazaro A, Cereijido M (1989) Establishment of tight junctions between cells from different animal species and different sealing capacities. J Membr Biol 107: 43-56.
26. Koljonen M, Hakala KS, Ahtola-Satila T, Laitinen L, Kostiainen R, et al. (2006) Evaluation of cocktail approach to standardise Caco-2 permeability experiments. Eur J Pharm Biopharm 64: 379-387. S0939-6411(06)00157-3 [pii];10.1016/j.ejpb.2006.06.006 [doi].
27. Overgaard CE, Mitchell LA, Koval M (2012) Roles for claudins in alveolar epithelial barrier function. Ann N Y Acad Sci 1257: 167-174. 10.1111/j.1749-6632.2012.06545.x [doi].
28. Milatz S, Krug SM, Rosenthal R, Gunzel D, Muller D, et al. (2010) Claudin-3 acts as a sealing component of the tight junction for ions of either charge and uncharged solutes. Biochim Biophys Acta 1798: 2048-2057. S0005-2736(10)00248-8 [pii];10.1016/j.bbamem.2010.07.014 [doi].
29. Nilsson HE, Dragomir A, Lazorova L, Johannesson M, Roomans GM (2010) CFTR and tight junctions in cultured bronchial epithelial cells. Exp Mol Pathol 88: 118-127. S0014-4800(09)00131-2 [pii];10.1016/j.yexmp.2009.09.018 [doi].
30. Lesimple P, Liao J, Robert R, Gruenert DC, Hanrahan JW (2010) Cystic fibrosis transmembrane conductance regulator trafficking modulates the barrier function of airway epithelial cell monolayers. J Physiol 588: 1195-1209. jphysiol.2009.182246 [pii];10.1113/jphysiol.2009.182246 [doi].
31. Schwiebert EM, Benos DJ, Egan ME, Stutts MJ, Guggino WB (1999) CFTR is a conductance regulator as well as a chloride channel. Physiol Rev 79: S145-S166.
32. Barnes LM, Bentley CM, Dickson AJ (2003) Stability of protein production from recombinant mammalian cells. Biotechnol Bioeng 81: 631-639. 10.1002/bit.10517 [doi].
33. Konishi Y, Hagiwara K, Shimizu M (2002) Transepithelial transport of fluorescein in Caco-2 cell monolayers and use of such transport in in vitro evaluation of phenolic acid availability. Biosci Biotechnol Biochem 66: 2449-2457.
34. Kuwayama K, Miyauchi S, Tateoka R, Abe H, Kamo N (2002) Fluorescein uptake by a monocarboxylic acid transporter in human intestinal Caco-2 cells. Biochem Pharmacol 63: 81-88. S000629520100853X [pii].
35. Soini Y (2011) Claudins in lung diseases. Respir Res 12: 70-81.
36. Coyne CB, Gambling TM, Boucher RC, Carson JL, Johnson LG (2003) Role of claudin interactions in airway tight junctional permeability. Am J Physiol Lung Cell Mol Physiol 285: L1166-L1178.
37. Lin X, Shang X, Manorek G, Howell SB (2013) Regulation of the Epithelial-Mesenchymal Transition by Claudin-3 and Claudin-4. PLoS One 8: e67496. 10.1371/journal.pone.0067496 [doi];PONE-D-13-00995 [pii].