SCOPUS 정보 검색 플랫폼

Volumn 12, Issue 27, 2014, Pages 5023-5030

A colorimetric and ratiometric fluorescent probe for distinguishing cysteine from biothiols in water and living cells

(11) Han, Qingxin a Shi, Zhaohua a Tang, Xiaoliang a Yang, Lizi a Mou, Zuolin a Li, Jing b Shi, Jinmin a Chen, Chunyang a Liu, Wei a Yang, Huan a Liu, Weisheng a

a LANZHOU UNIVERSITY (China)

b LANZHOU UNIVERSITY (China)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CHROMOPHORES; COLOR; FLUORESCENCE; PROBES;

AQUEOUS BUFFER SOLUTION; CELL PERMEABILITY; CHROMOPHORE GROUP; ELECTRON SYSTEMS; FLUORESCENT PROBES; INTRAMOLECULAR CHARGE TRANSFERS; LOW CYTOTOXICITIES; RATIOMETRIC FLUORESCENCE;

COLORIMETRY;

CYSTEINE; FLUORESCENT DYE; WATER;

BLOOD; CELL SURVIVAL; CHEMISTRY; COLORIMETRY; DRUG STABILITY; FLUORESCENCE MICROSCOPY; HELA CELL LINE; HUMAN; KINETICS; PH; RADIOMETRY; SPECTROFLUOROMETRY; SYNTHESIS;

CELL SURVIVAL; COLORIMETRY; CYSTEINE; DRUG STABILITY; FLUORESCENT DYES; HELA CELLS; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; MICROSCOPY, FLUORESCENCE; RADIOMETRY; SPECTROMETRY, FLUORESCENCE; WATER;

EID: 84902812430 PISSN: 14770520 EISSN: None Source Type: Journal
DOI: 10.1039/c4ob00463a Document Type: Article

Times cited : (50)

References (65)

1
- 0037222255
- Z. A. Wood E. Schröder J. Robin Harris L. B. Poole Trends Biochem. Sci. 2003 28 32 40
- (2003) Trends Biochem. Sci. , vol.28 , pp. 32-40
- Wood, Z.A.¹ Schröder, E.² Robin Harris, J.³ Poole, L.B.⁴

2
- 0033899208
- J. B. Schulz J. Lindenau J. Seyfried J. Dichgans Eur. J. Biochem. 2000 267 4904 4911
- (2000) Eur. J. Biochem. , vol.267 , pp. 4904-4911
- Schulz, J.B.¹ Lindenau, J.² Seyfried, J.³ Dichgans, J.⁴

3
- 0027620493
- T. Wlostowski BioMetals 1993 6 71 76
- (1993) BioMetals , vol.6 , pp. 71-76
- Wlostowski, T.¹

4
- 0035873398
- X. F. Wang M. S. Cynader J. Neurosci. 2001 21 3322 3331
- (2001) J. Neurosci. , vol.21 , pp. 3322-3331
- Wang, X.F.¹ Cynader, M.S.²

5
- 0035894261
- S. Shahrokhian Anal. Chem. 2001 73 5972 5978
- (2001) Anal. Chem. , vol.73 , pp. 5972-5978
- Shahrokhian, S.¹

6
- 78650078496
- E. Weerapana C. Wang G. M. Simon F. Richter S. Khare M. B. Dillon D. A. Bachovchin K. Mowen D. Baker B. F. Cravatt Nature 2010 468 790 795
- (2010) Nature , vol.468 , pp. 790-795
- Weerapana, E.¹ Wang, C.² Simon, G.M.³ Richter, F.⁴ Khare, S.⁵ Dillon, M.B.⁶ Bachovchin, D.A.⁷ Mowen, K.⁸ Baker, D.⁹ Cravatt, B.F.¹⁰

7
- 84878018203
- L. L. Gluud G. Dam M. Borre I. Les J. Cordoba G. Marchesini N. K. Aagaard H. Vilstrup Metab. Brain Dis. 2013 28 221 225
- (2013) Metab. Brain Dis. , vol.28 , pp. 221-225
- Gluud, L.L.¹ Dam, G.² Borre, M.³ Les, I.⁴ Cordoba, J.⁵ Marchesini, G.⁶ Aagaard, N.K.⁷ Vilstrup, H.⁸

8
- 0037733975
- D. M. Townsend K. D. Tew H. Tapiero Biomed. Pharmacother. 2003 57 145 155
- (2003) Biomed. Pharmacother. , vol.57 , pp. 145-155
- Townsend, D.M.¹ Tew, K.D.² Tapiero, H.³

9
- 57549095616
- K. G. Reddie K. S. Carroll Curr. Opin. Chem. Biol. 2008 12 746 754
- (2008) Curr. Opin. Chem. Biol. , vol.12 , pp. 746-754
- Reddie, K.G.¹ Carroll, K.S.²

10
- 84870168520
- J. Chan S. C. Dodani C. J. Chang Nat. Chem. 2012 4 973 984
- (2012) Nat. Chem. , vol.4 , pp. 973-984
- Chan, J.¹ Dodani, S.C.² Chang, C.J.³

11
- 79952592188
- H. S. Jung K. C. Ko G. H. Kim A. R. Lee Y. C. Na C. Kang J. Y. Lee J. S. Kim Org. Lett. 2011 13 1498 1501
- (2011) Org. Lett. , vol.13 , pp. 1498-1501
- Jung, H.S.¹ Ko, K.C.² Kim, G.H.³ Lee, A.R.⁴ Na, Y.C.⁵ Kang, C.⁶ Lee, J.Y.⁷ Kim, J.S.⁸

12
- 81055123737
- L. Deng W. Wu H. Guo J. Zhao S. Ji X. Zhang X. Yuan C. Zhang J. Org. Chem. 2011 76 9294 9304
- (2011) J. Org. Chem. , vol.76 , pp. 9294-9304
- Deng, L.¹ Wu, W.² Guo, H.³ Zhao, J.⁴ Ji, S.⁵ Zhang, X.⁶ Yuan, X.⁷ Zhang, C.⁸

13
- 84863523381
- B. K. McMahon T. Gunnlaugsson J. Am. Chem. Soc. 2012 134 10725 10728
- (2012) J. Am. Chem. Soc. , vol.134 , pp. 10725-10728
- McMahon, B.K.¹ Gunnlaugsson, T.²

14
- 67651213457
- V. Hong A. A. Kislukhin M. G. Finn J. Am. Chem. Soc. 2009 131 9986 9994
- (2009) J. Am. Chem. Soc. , vol.131 , pp. 9986-9994
- Hong, V.¹ Kislukhin, A.A.² Finn, M.G.³

15
- 70349143182
- H. S. Hewage E. V. Anslyn J. Am. Chem. Soc. 2009 131 13099 13106
- (2009) J. Am. Chem. Soc. , vol.131 , pp. 13099-13106
- Hewage, H.S.¹ Anslyn, E.V.²

16
- 66149152219
- W. Lin L. Yuan Z. Cao Y. Feng L. Long Chem.-Eur. J. 2009 15 5096 5103
- (2009) Chem.-Eur. J. , vol.15 , pp. 5096-5103
- Lin, W.¹ Yuan, L.² Cao, Z.³ Feng, Y.⁴ Long, L.⁵

17
- 79251488802
- H. Kwon K. Lee H. J. Kim Chem. Commun. 2011 47 1773 1775
- (2011) Chem. Commun. , vol.47 , pp. 1773-1775
- Kwon, H.¹ Lee, K.² Kim, H.J.³

18
- 77950504281
- X. Chen S. K. Ko M. J. Kim I. Shin J. Yoon Chem. Commun. 2010 46 2751 2753
- (2010) Chem. Commun. , vol.46 , pp. 2751-2753
- Chen, X.¹ Ko, S.K.² Kim, M.J.³ Shin, I.⁴ Yoon, J.⁵

19
- 70349967914
- L. Yi H. Li L. Sun L. Liu C. Zhang Z. Xi Angew. Chem., Int. Ed. 2009 48 4034 4037
- (2009) Angew. Chem., Int. Ed. , vol.48 , pp. 4034-4037
- Yi, L.¹ Li, H.² Sun, L.³ Liu, L.⁴ Zhang, C.⁵ Xi, Z.⁶

20
- 54749131828
- S. Sreejith K. P. Divya A. Ajayaghosh Angew. Chem., Int. Ed. 2008 47 7883 7887
- (2008) Angew. Chem., Int. Ed. , vol.47 , pp. 7883-7887
- Sreejith, S.¹ Divya, K.P.² Ajayaghosh, A.³

21
- 77950504281
- X. Chen S.-K. Ko M. J. Kim I. Shin J. Yoon Chem. Commun. 2010 46 2751 2753
- (2010) Chem. Commun. , vol.46 , pp. 2751-2753
- Chen, X.¹ Ko, S.-K.² Kim, M.J.³ Shin, I.⁴ Yoon, J.⁵

22
- 64049087040
- X. Zhang X. Ren Q. H. Xu K. P. Loh Z. K. Chen Org. Lett. 2009 11 1257 1260
- (2009) Org. Lett. , vol.11 , pp. 1257-1260
- Zhang, X.¹ Ren, X.² Xu, Q.H.³ Loh, K.P.⁴ Chen, Z.K.⁵

23
- 60949113101
- W. Lin L. Long L. Yuan Z. Cao B. Chen W. Tan Org. Lett. 2008 10 5577 5580
- (2008) Org. Lett. , vol.10 , pp. 5577-5580
- Lin, W.¹ Long, L.² Yuan, L.³ Cao, Z.⁴ Chen, B.⁵ Tan, W.⁶

24
- 77955435308
- L. Xiong Q. Zhao H. Chen Y. Wu Z. Dong Z. Zhou F. Li Inorg. Chem. 2010 49 6402 6408
- (2010) Inorg. Chem. , vol.49 , pp. 6402-6408
- Xiong, L.¹ Zhao, Q.² Chen, H.³ Wu, Y.⁴ Dong, Z.⁵ Zhou, Z.⁶ Li, F.⁷

25
- 56849126506
- K. S. Lee T. K. Kim J. H. Lee H. J. Kim J. I. Hong Chem. Commun. 2008 6173 6175
- (2008) Chem. Commun. , pp. 6173-6175
- Lee, K.S.¹ Kim, T.K.² Lee, J.H.³ Kim, H.J.⁴ Hong, J.I.⁵

26
- 43549112613
- M. M. Pires J. Chmielewski Org. Lett. 2008 10 837 840
- (2008) Org. Lett. , vol.10 , pp. 837-840
- Pires, M.M.¹ Chmielewski, J.²

27
- 38349152249
- J. Bouffard Y. Kim T. M. Swager R. Weissleder S. A. Hilderbrand Org. Lett. 2008 10 37 40
- (2008) Org. Lett. , vol.10 , pp. 37-40
- Bouffard, J.¹ Kim, Y.² Swager, T.M.³ Weissleder, R.⁴ Hilderbrand, S.A.⁵

28
- 77955120032
- X. Li S. Qian Q. He B. Yang J. Li Y. Hu Org. Biomol. Chem. 2010 8 3627 3630
- (2010) Org. Biomol. Chem. , vol.8 , pp. 3627-3630
- Li, X.¹ Qian, S.² He, Q.³ Yang, B.⁴ Li, J.⁵ Hu, Y.⁶

29
- 67649580270
- S. Ji J. Yang Q. Yang S. Liu M. Chen J. Zhao J. Org. Chem. 2009 74 4855 4865
- (2009) J. Org. Chem. , vol.74 , pp. 4855-4865
- Ji, S.¹ Yang, J.² Yang, Q.³ Liu, S.⁴ Chen, M.⁵ Zhao, J.⁶

30
- 80052774301
- X. Cao W. Lin Q. Yu J. Org. Chem. 2011 76 7423 7430
- (2011) J. Org. Chem. , vol.76 , pp. 7423-7430
- Cao, X.¹ Lin, W.² Yu, Q.³

31
- 34748883933
- B. Tang Y. Xing P. Li N. Zhang F. Yu G. Yang J. Am. Chem. Soc. 2007 129 11666 11667
- (2007) J. Am. Chem. Soc. , vol.129 , pp. 11666-11667
- Tang, B.¹ Xing, Y.² Li, P.³ Zhang, N.⁴ Yu, F.⁵ Yang, G.⁶

32
- 79960607599
- C. S. Lim G. Masanta H. J. Kim J. H. Han H. M. Kim B. R. Cho J. Am. Chem. Soc. 2011 133 11132 11135
- (2011) J. Am. Chem. Soc. , vol.133 , pp. 11132-11135
- Lim, C.S.¹ Masanta, G.² Kim, H.J.³ Han, J.H.⁴ Kim, H.M.⁵ Cho, B.R.⁶

33
- 84862969080
- M. H. Lee J. H. Han P. S. Kwon S. Bhuniya J. Y. Kim J. L. Sessler C. Kang J. S. Kim J. Am. Chem. Soc. 2012 134 1316 1322
- (2012) J. Am. Chem. Soc. , vol.134 , pp. 1316-1322
- Lee, M.H.¹ Han, J.H.² Kwon, P.S.³ Bhuniya, S.⁴ Kim, J.Y.⁵ Sessler, J.L.⁶ Kang, C.⁷ Kim, J.S.⁸

34
- 77950394155
- J. H. Lee C. S. Lim Y. S. Tian J. H. Han B. R. Cho J. Am. Chem. Soc. 2010 132 1216 1217
- (2010) J. Am. Chem. Soc. , vol.132 , pp. 1216-1217
- Lee, J.H.¹ Lim, C.S.² Tian, Y.S.³ Han, J.H.⁴ Cho, B.R.⁵

35
- 84863229492
- J. Shao H. Sun H. Guo S. Ji J. Zhao W. Wu X. Yuan C. Zhang T. D. James Chem. Sci. 2012 3 1049
- (2012) Chem. Sci. , vol.3 , pp. 1049
- Shao, J.¹ Sun, H.² Guo, H.³ Ji, S.⁴ Zhao, J.⁵ Wu, W.⁶ Yuan, X.⁷ Zhang, C.⁸ James, T.D.⁹

36
- 77955224668
- B. Zhu X. Zhang Y. Li P. Wang H. Zhang X. Zhuang Chem. Commun. 2010 46 5710 5712
- (2010) Chem. Commun. , vol.46 , pp. 5710-5712
- Zhu, B.¹ Zhang, X.² Li, Y.³ Wang, P.⁴ Zhang, H.⁵ Zhuang, X.⁶

37
- 78650485317
- L. Long W. Lin B. Chen W. Gao L. Yuan Chem. Commun. 2011 47 893 895
- (2011) Chem. Commun. , vol.47 , pp. 893-895
- Long, L.¹ Lin, W.² Chen, B.³ Gao, W.⁴ Yuan, L.⁵

38
- 34548249623
- M. Zhang M. Yu F. Li M. Zhu M. Li Y. Gao L. Li Z. Liu J. Zhang D. Zhang T. Yi C. Huang J. Am. Chem. Soc. 2007 129 10322 10323
- (2007) J. Am. Chem. Soc. , vol.129 , pp. 10322-10323
- Zhang, M.¹ Yu, M.² Li, F.³ Zhu, M.⁴ Li, M.⁵ Gao, Y.⁶ Li, L.⁷ Liu, Z.⁸ Zhang, J.⁹ Zhang, D.¹⁰ Yi, T.¹¹ Huang, C.¹²

39
- 34247538391
- Y. H. Ahn J. S. Lee Y. T. Chang J. Am. Chem. Soc. 2007 129 4510 4511
- (2007) J. Am. Chem. Soc. , vol.129 , pp. 4510-4511
- Ahn, Y.H.¹ Lee, J.S.² Chang, Y.T.³

40
- 77949299014
- H. Y. Shiu H. C. Chong Y. C. Leung M. K. Wong C. M. Che Chem.-Eur. J. 2010 16 3308 3313
- (2010) Chem.-Eur. J. , vol.16 , pp. 3308-3313
- Shiu, H.Y.¹ Chong, H.C.² Leung, Y.C.³ Wong, M.K.⁴ Che, C.M.⁵

41
- 77957851209
- Y. K. Yang S. Shim J. Tae Chem. Commun. 2010 46 7766 7768
- (2010) Chem. Commun. , vol.46 , pp. 7766-7768
- Yang, Y.K.¹ Shim, S.² Tae, J.³

42
- 79954627437
- H. S. Jung J. H. Han Y. Habata C. Kang J. S. Kim Chem. Commun. 2011 47 5142 5144
- (2011) Chem. Commun. , vol.47 , pp. 5142-5144
- Jung, H.S.¹ Han, J.H.² Habata, Y.³ Kang, C.⁴ Kim, J.S.⁵

43
- 84884767942
- J. Shi Y. Wang X. Tang W. Liu H. Jiang W. Dou W. Liu Dyes Pigm. 2014 100 255 260
- (2014) Dyes Pigm. , vol.100 , pp. 255-260
- Shi, J.¹ Wang, Y.² Tang, X.³ Liu, W.⁴ Jiang, H.⁵ Dou, W.⁶ Liu, W.⁷

44
- 70349659727
- H. Li J. Fan J. Wang M. Tian J. Du S. Sun P. Sun X. Peng Chem. Commun. 2009 5904 5906
- (2009) Chem. Commun. , pp. 5904-5906
- Li, H.¹ Fan, J.² Wang, J.³ Tian, M.⁴ Du, J.⁵ Sun, S.⁶ Sun, P.⁷ Peng, X.⁸

45
- 80155143389
- X. Yang Y. Guo R. M. Strongin Angew. Chem., Int. Ed. 2011 50 10690 10693
- (2011) Angew. Chem., Int. Ed. , vol.50 , pp. 10690-10693
- Yang, X.¹ Guo, Y.² Strongin, R.M.³

46
- 79957453164
- L. Yuan W. Lin Y. Yang Chem. Commun. 2011 47 6275 6277
- (2011) Chem. Commun. , vol.47 , pp. 6275-6277
- Yuan, L.¹ Lin, W.² Yang, Y.³

47
- 84864481860
- Z. Guo S. Nam S. Park J. Yoon Chem. Sci. 2012 3 2760
- (2012) Chem. Sci. , vol.3 , pp. 2760
- Guo, Z.¹ Nam, S.² Park, S.³ Yoon, J.⁴

48
- 81155152759
- H. S. Jung J. H. Han T. Pradhan S. Kim S. W. Lee J. L. Sessler T. W. Kim C. Kang J. S. Kim Biomaterials 2012 33 945 953
- (2012) Biomaterials , vol.33 , pp. 945-953
- Jung, H.S.¹ Han, J.H.² Pradhan, T.³ Kim, S.⁴ Lee, S.W.⁵ Sessler, J.L.⁶ Kim, T.W.⁷ Kang, C.⁸ Kim, J.S.⁹

49
- 84878598954
- X. Zhou X. Jin G. Sun X. Wu Chem.-Eur. J. 2013 19 7817 7824
- (2013) Chem.-Eur. J. , vol.19 , pp. 7817-7824
- Zhou, X.¹ Jin, X.² Sun, G.³ Wu, X.⁴

50
- 84863724710
- X. Yang Y. Guo R. M. Strongin Org. Biomol. Chem. 2012 10 2739 2741
- (2012) Org. Biomol. Chem. , vol.10 , pp. 2739-2741
- Yang, X.¹ Guo, Y.² Strongin, R.M.³

51
- 70349269188
- S. P. Wang W. J. Deng D. Sun M. Yan H. Zheng J. G. Xu Org. Biomol. Chem. 2009 7 4017 4020
- (2009) Org. Biomol. Chem. , vol.7 , pp. 4017-4020
- Wang, S.P.¹ Deng, W.J.² Sun, D.³ Yan, M.⁴ Zheng, H.⁵ Xu, J.G.⁶

52
- 84880855383
- J. Chang Y. Lu S. He C. Liu L. Zhao X. Zeng Chem. Commun. 2013 49 6259 6261
- (2013) Chem. Commun. , vol.49 , pp. 6259-6261
- Chang, J.¹ Lu, Y.² He, S.³ Liu, C.⁴ Zhao, L.⁵ Zeng, X.⁶

53
- 0013984616
- N. J. Leonard R. Y. Ning J. Org. Chem. 1966 31 3928 3935
- (1966) J. Org. Chem. , vol.31 , pp. 3928-3935
- Leonard, N.J.¹ Ning, R.Y.²

54
- 0002460703
- G. Illuminati L. Mandolini Acc. Chem. Res. 1981 14 95 102
- (1981) Acc. Chem. Res. , vol.14 , pp. 95-102
- Illuminati, G.¹ Mandolini, L.²

55
- 0000079249
- C. Galli G. Illuminati L. Mandolini P. Tamborra J. Am. Chem. Soc. 1977 99 2591 2597
- (1977) J. Am. Chem. Soc. , vol.99 , pp. 2591-2597
- Galli, C.¹ Illuminati, G.² Mandolini, L.³ Tamborra, P.⁴

56
- 84870248583
- J. Gu U. R. Anumala F. Lo Monte T. Kramer R. Heyny von Haussen J. Holzer V. Goetschy-Meyer G. Mall I. Hilger C. Czech B. Schmidt Bioorg. Med. Chem. Lett. 2012 22 7667 7671
- (2012) Bioorg. Med. Chem. Lett. , vol.22 , pp. 7667-7671
- Gu, J.¹ Anumala, U.R.² Lo Monte, F.³ Kramer, T.⁴ Heyny Von Haussen, R.⁵ Holzer, J.⁶ Goetschy-Meyer, V.⁷ Mall, G.⁸ Hilger, I.⁹ Czech, C.¹⁰ Schmidt, B.¹¹

57
- 0029974518
- J. Ma G. Stoter J. Verweij J. H. M. Schellens Cancer Chemother. Pharmacol. 1996 38 391 394
- (1996) Cancer Chemother. Pharmacol. , vol.38 , pp. 391-394
- Ma, J.¹ Stoter, G.² Verweij, J.³ Schellens, J.H.M.⁴

58
- 0037075257
- S. Seshadri A. Beiser J. Selhub P. F. Jacques I. H. Rosenberg R. B. D'Agostino P. W. F. Wilson P. A. Wolf N. Engl. J. Med. 2002 346 476 483
- (2002) N. Engl. J. Med. , vol.346 , pp. 476-483
- Seshadri, S.¹ Beiser, A.² Selhub, J.³ Jacques, P.F.⁴ Rosenberg, I.H.⁵ D'Agostino, R.B.⁶ Wilson, P.W.F.⁷ Wolf, P.A.⁸

59
- 0031958860
- M. H. Refsum M. P. M. Ueland M. O. Nyg ård M. D. D. P. H. S. E. Vollset Annu. Rev. Med. 1998 49 31 62
- (1998) Annu. Rev. Med. , vol.49 , pp. 31-62
- Refsum, M.H.¹ Ueland, M.P.M.² Nyg Ård, M.O.³ Vollset, M.D.D.P.H.S.E.⁴

60
- 0021819571
- E. Beutler T. Gelbart J. Lab. Clin. Med. 1985 105 581 584
- (1985) J. Lab. Clin. Med. , vol.105 , pp. 581-584
- Beutler, E.¹ Gelbart, T.²

61
- 0346456896
- O. Rusin N. N. St. Luce R. A. Agbaria J. O. Escobedo S. Jiang I. M. Warner F. B. Dawan K. Lian R. M. Strongin J. Am. Chem. Soc. 2003 126 438 439
- (2003) J. Am. Chem. Soc. , vol.126 , pp. 438-439
- Rusin, O.¹ St. Luce, N.N.² Agbaria, R.A.³ Escobedo, J.O.⁴ Jiang, S.⁵ Warner, I.M.⁶ Dawan, F.B.⁷ Lian, K.⁸ Strongin, R.M.⁹

62
- 0345491105
- C. Lee W. Yang R. G. Parr Phys. Rev. B: Condens. Matter 1988 37 785 789
- (1988) Phys. Rev. B: Condens. Matter , vol.37 , pp. 785-789
- Lee, C.¹ Yang, W.² Parr, R.G.³

63
- 0000189651
- A. D. Becke J. Chem. Phys. 1993 98 5648
- (1993) J. Chem. Phys. , vol.98 , pp. 5648
- Becke, A.D.¹

64
- 33745770836
- P. J. Hay W. R. Wadt J. Chem. Phys. 1985 82 270
- (1985) J. Chem. Phys. , vol.82 , pp. 270
- Hay, P.J.¹ Wadt, W.R.²

65
- 27344448074
- P. J. Hay W. R. Wadt J. Chem. Phys. 1985 82 299
- (1985) J. Chem. Phys. , vol.82 , pp. 299
- Hay, P.J.¹ Wadt, W.R.²

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.