Conventional and alternative technologies for the extraction of algal polysaccharides

Functional Ingredients from Algae for Foods and Nutraceuticals

Volumn , Issue , 2013, Pages 475-516

  Hernandez Carmona G ^a   Freile Pelegrin Y ^b   Hernandez Garibay E ^c  

^a INSTITUTO POLITÉCNICO NACIONAL   (Mexico)

^b DEPARTAMENTO DE FÍSICA   (Mexico)

^c Instituto nacional de Pesca (INAPESCA)   (Mexico)

Author keywords

Agar; Alginate; Carrageenan; Phycocolloid process; Seaweeds polysaccharides

Indexed keywords

EID: 84902618695     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1533/9780857098689.3.475     Document Type: Chapter

References (118)

1. Abreu M.H., Pereira R., Yarish C.H., Buschmann A., Sousa-Pinto I. IMTA with Gracilaria vermiculophylla: productivity and nutrient removal performance of the seaweed in a land-based pilot scale system. Aquaculture 2011, 312:77-87.
2. Alireza-Razavi A., Khodadadi A., Bagher-Eslami M., Eshraghi S., Mirshafiey A. Therapeutic effect of sodium alginate in experimental chronic ulcerative colitis. Iranian Journal of Allergy, Asthma and Immunology 2008, 7(1):13-18.
3. Armisén R. World-wide use and importance of Gracilaria. Journal of Applied Phycology 1995, 7(3):231-243.
4. Armisén A.R. Ficocoloides. Polisacáridos de las algas marinas 2000, Madrid (España) (Ed.).
5. Armisén R., Galatas F. Production, properties and uses of agar. Production and Utilization of Products from Commercial Seaweeds 1987, FAO Fisheries Technical Papers 288, Rome. D.J. McHugh (Ed.).
6. Armisen R., Galatas F. Agar. Handbook of Hydrocolloids 2000, CRC, Boca Raton, Florida. G. Phillips, P. Williams (Eds.).
7. Arvizu-Higuera D.L., Hernández-Carmona G., Rodríguez-Montesinos Y.E. Batch and continuous flow system during the acid pre-extraction stage in the alginate extraction process. Ciencias Marinas 1995, 21(1):25-37.
8. Arvizu-Higuera D.L., Hernández-Carmona G., Rodríguez-Montesinos Y.E. Effect of temperature and extraction time on the process to obtain alginate from Macrocystis pyrifera. Ciencias Marinas 1996, 22(4):511-521.
9. Arvizu-Higuera D.L., Hernández-Carmona G., Rodríguez-Montesinos Y.E. Effect of the type of precipitation on the process to obtain sodium alginate: calcium alginate method and alginic acid method. Ciencias Marinas 1997, 23(2):195-207.
10. Arvizu-Higuera D.L., Hernández-Carmona G., Rodríguez-Montesinos Y.E. Parameters afecting the conversion of alginic acid to sodium alginate. Ciencias Marinas 2002, 28(1):27-36.
11. Ayal H.A., Matsuhiro B. Polysaccharides from nuclear phases of Iridaea ciliata and I. membranacea. Hidrobiologia 1987, 151/152:531-534.
12. Bellion C., Brigand G., Prome J.C., Welti D., Bociek S. Identification et caractérisation des précurseurs biologiques des carraghénanes par spectroscopie de RMN 13C. Carbohydrate Research 1983, 119:31-48.
13. Bixler H. Recent developments in manufacturing and marketing carrageenan. Hydrobiologia 1996, 326(32):735-737.
14. Bixler H.J., Porse H. A decade of change in the seaweed hydrocolloids industry. Journal of Applied Phycology 2011, 23:321-335.
15. Calumpong H.P., Maypa A.P., Magbanua M. Population and alginate yield and quality assessment of four Sargassum species in Negros Island, central Philippines. Hydrobiologia 1999, 398(399):211-215.
16. Chang P.R., Yu J., Ma X., Anderson D.P. Polysaccharides as stabilizers for the synthesis of magnetic nanoparticles. Carbohydrate Polymers 2011, 83:640-644.
17. Chopin T., Buschmann A.H., Hallin C., Troell M., Kautsky N., Neori A., Kraemer G.P., Zertuche-Gonzalez J.A., Neefus C. Integrating seaweeds into marine aquaculture systems: a key toward sustainability. Journal of Applied Phycology 2001, 37:975-986.
18. Coppi G., Iannuccelli V., Leo E., Bernabei M.T., Cameroni R. Chitosan-Alginate microparticles as a protein carrier. Drug Development and Industrial Pharmacy 2001, 27(5):393-400.
19. Craigie J. Cell wall. Biology of the Red Algae 1990, Cambridge University Press, Cambridge. K.M. Cole, R.G. Sheath (Eds.).
20. Davis T.A., Ramirez M., Mucci A., Larsen B. Extraction, isolation and cadmium binding of alginate from Sargassum spp. Journal of Applied Phycology 2004, 16(4):275-284.
21. Dawes C.J., Stanley N.F., Stancioff D.J. Seasonal and reproductive aspects of plant chemistry and ι-carrageenan from Floridean Eucheuma (Rhodophyta Gigartinales). Botanica Marina 1977, 20:137-147.
22. De Stefano C., Gianguzza A., Piazzese D., Sammartano S. Modelling of proton and metal exchange in the alginate biopolymer. Analytical and Bioanalytical Chemistry 2005, 383(4):587-596.
23. Deslandes E., Floćch J.Y., Bodeau-Bellion C., Brault D., Braud J.P. Evidence for ι-carrageenans in Solieria chordialis (Solieriaceae) and Calliblepharis jubata, Calliblepharis ciliata, Cystoclonium purpureum (Rhodophyllidaceae). Botanica Marina 1985, 28:317-318.
24. Draget K.I., Smidsrod O., Skjak-Braek G. Alginates from algae. Polysaccharides and Polyamides in the Food Industry: Properties, Production and Patents I 2005, Wiley-VCH, Weinheim. A. Steinbüchel, S.K. Rhee (Eds.).
25. Fenoradosoa T.A., Ali G., Delattre C., Laroche C., Petit E., Wadouachi A., Michaud P. Extraction and characterization of an alginate from the brown seaweed Sargassum turbinarioides Grunow. Journal of Applied Phycology 2010, 22:131-137.
26. Food Chemicals Codes 1981, National Academy Press, Washington, DC. Third Edition.
27. Freile-Pelegrin Y., Murano E. Agars from three species of Gracilaria (Rhodophyta) from Yucatán Peninsula. Bioresource Technology 2005, 96:295-302.
28. Freile-Pelegrin Y., Robledo D. Influence of alkali treatment on agar from Gracilaria cornea from Yucatán Mexico. Journal of Applied Phycology 1997, 9:533-539.
29. Freile-Pelegrin Y., Robledo D., Pedersén M., Bruno E., Rönnqvist J. Effect of dark and salinity treatment in the yield and quality of agar from Gracilaria cornea (Rhodophyceae). Ciencias Marinas 2002, 28(3):289-296.
30. Fromholt L.P. Carrageenan-containing product and a method of producing same 1998, US Patent No. 5,777,102.
31. Gao H.Z., Zepp R.G. Factors influencing photoreactions of dissolved organic matter in coastal river of the southern United States. Environmental Science Technology 1998, 32:2940-2946.
32. Gorenšek M., Bukošek V. Zinc and alginate for multipurpose textiles. Acta Chimica Slovenica 2006, 53(2):223-228.
33. Greer C.W., Yaphe W. Characterization of hybrid (beta-kappa-gamma) carrageenan from Eucheuma gelatinae J Agardh (Rhodophyta, Solieriaceae) using carrageenases, infrared and 13C-nuclear magnetic resonance spectroscopy. Botanica Marina 1984, 27:473-478.
34. Guiseley K.B. The relationship between methoxyl content and gelling temperature of agarose. Carbohydrate Research 1970, 13:247-256.
35. Hansen M. E for additives 1987, Thorson Publishing Group, Wellingborough.
36. Haug A. Composition and properties of alginates 1964, Report 30, Norwegian Institute Seaweed Research, Throndheim, Norway.
37. Haug A., Larsen B., Smidsrod O. A study of the constitution of alginic acid by partial acid hydrolysis. Acta Chemica Scandinavica 1966, 20:183-190.
38. Hernández-Carmona G., Aguirre-Vilchis M. Propiedades de intercambio iónico de Macrocystis pyrifera durante la pre-extracción ácida, para la extracción de alginatos. Investigaciones Marinas CICIMAR 1987, 3(2):53-64.
39. Hernández-Carmona G., Casas-Valdez M.M. Precipitación del ácido algínico y su conversión a alginato de sodio en muestras de Macrocystis pyrifera. Investigaciones Marinas CICIMAR 1985, 2(1):18-28.
40. Hernández-Carmona G., McHugh D.J., Arvizu-Higuera D.L., Rodríguez-Montesinos Y.E. Pilot plant scale extraction of alginate from Macrocystis pyrifera. Part 1. The effect of pre-extraction treatments on the yield and quality of alginate. Journal of Applied Phycology 1999, 10(6):507-513.
41. Hernández-Carmona G., McHugh D.J., Arvizu-Higuera D.L., Rodríguez-Montesinos Y.E. Pilot plant scale extraction of alginates from Macrocystis pyrifera 4. Conversion of alginic acid to sodium alginate, drying and milling. Journal of Applied Phycology 2002, 14(6):445-451.
42. Hernández-Carmona G., McHugh D.J., López-Gutiérrez F. Pilot plant scale extraction of alginates from Macrocystis pyrifera. 2. Studies on extraction conditions and methods of separating the alkaline-insoluble residue. Journal of Applied Phycology 1999, 11(6):493-502.
43. Horn S.J., Aasen I.M., Østgaard K. Ethanol production from seaweed extract. Journal of Industrial Microbiology and Biotechnology 2000, 25:249-254.
44. 4 magnetic nanoparticles. Nanotechnology 2010, vol. 21:445601. Epub 2010 Oct 8.
45. Imeson A.P. Carrageenan. Handbook of Hydrocolloids 2000, 87-102. Woodhead Publishing, Cambridge. G.O. Phillips, P.A. Williams (Eds.).
46. Imeson A.P., Ledward D.A., Mitchell J.R. On the nature of the interaction between some anionic polysaccharides and proteins. Journal of the Science of Food and Agriculture 1977, 28:661-667.
47. Istini S.O., Kusunose M. Methods of analysis of agar, carrageenan and alginates in seaweeds', Bulletin of Marine Science and Fisheries. Kochi University 1994, vol. 14:49-55.
48. Iwasaki K., Matsubara Y. Purification of alginate oligosaccharides with root growth-promoting activity toward lettuce. Bioscience Biotechnology & Biochemistry 2000, 64:1067-1070.
49. Jin Y., Li H.Y., Zhang W., Cong X.J., Liu Y.L. Development of an eco-friendly agar extraction technique from the red seaweed Gracilaria spp 2006, Chinese patent No. 200610047762.2.
50. Kelco Alginate products for scientific water control 1996, 1-33. USA.
51. Kluck J.H. Microwave apparatus for rapid heating of fluids 1970, US Patent N. 3535482.
52. Knutsen S.H., Myslabodski D.E., Larsen B., Usov A.I. A modified system of nomenclature for red algal galactans. Botanica Marina 1994, 37:163-169.
53. Kronberga M., Karklina D., Murniece I., Kruma Z. Changes of agar-agar gel properties after replacing sucrose by inulin syrup. Innovations for Food Science and Production 2011, Latvia University of Agriculture, Jelgava, Latvia. E. Straumite (Ed.).
54. Lahaye M., Rochas C. Chemical structure and physico-chemical properties of agar. Hydrobiologia 1991, 221:137-148.
55. Li H.Y., Yu X.J., Jin Y., Zhang W., Liu Y.L. Development of an eco-friendly agar extraction technique from the red seaweed Gracilaria lemaneiformis. Bioresource Technology 2008, 99:3301-3305.
56. Lobban C.S., Harrison P.J. Seaweed Ecology and Physiology 1994, Cambridge University Press, Cambridge.
57. Madera-Santana T.J., Misra M., Drzal L.T., Robledo D., Freile-Pelegrin Y. Preparation and characterization of biodegradable agar/poly (butylene adipaterco-terephatalate) composites. Polymer Engineering and Science 2009, 49(6):1117-1126.
58. Madera-Santana T., Robledo D., Azamar J.A., Ríos-Soberanis C.R., Freile-Pelegrin Y. Preparation and characterization of low density polyethylene agar biocomposites: torque-rheological mechanical, thermal and morphological properties. Polymer Engineering and Science 2010, 50:585-591.
59. Madera-Santana T., Robledo D., Freile-Pelegrin Y. Physicochemical properties of biodegradable polyvinyl alcohol-agar films from the red algae Hydropuntia cornea. Marine Biotechnology 2011, 13:793-800.
60. Maeda H., Yamamoto R., Hirao K., Tochikubo O. Effects of agar (kanten) diet on obese patients with impaired glucose tolerance and type 2 diabetes. Diabetes Obesity and Metabolism 2005, 7(1):40-46.
61. Mandal V., Mohan Y., Hemalatha S. Microwave assisted extraction: an innovative and promising extraction tool for medicinal plant research. Pharmacognosy Reviews 2007, 1(1):7-18.
62. Matsuhashi T. Agar. Food Gels 1990, Elsevier Science Publishers Ltd., London. P. Harris (Ed.).
63. Matulewicz M.C., Ciancia M., Noseda M.D., Cerezo A.S. The carrageenan systems from tetrasporic and cystocarpic stages of Gigartina skottsbergii. Phytochemistry 1989, vol. 28(11):2937-2941.
64. McCandless E.L. The importance of cell wall constituents in algal taxonomy. Modern Approaches to the Taxonomy of Red and Brown Algae 1978, 63-85. London Academic Press. D.E.G. Irvine, J.H. Price (Eds.).
65. McCandless E.L., Craigie J.S., Walter J.E. Carrageenans in the gametophytic and sporophytic stages of Chondrus crispus. Planta (Berl) 1973, 112:201-212.
66. McCandless E.L., West J.A., Guiry M.D. Carrageenan patterns in the Gigartinaceae. Biochem System Ecol 1983, 11:175-182.
67. McHugh D.J. Production and utilization of products from commercial seaweeds 1987, FAO Fisheries Technical Paper 288, Roma.
68. McHugh D.J. A guide to the seaweed industry 2003, no. 441, FAO Fisheries Technical Paper, Rome.
69. McHugh D.J. The seaweed industry in the Pacific islands. ACIAR Working Paper No. 61 2006.
70. McHugh D.J., Hernández-Carmona G., Arvizu-Higuera D.L., Rodríguez Montesinos Y.E. Pilot plant scale extraction of alginates from Macrocystis pyrifera. 3. Precipitation, bleaching and conversion of calcium alginate to alginic acid. Journal of Applied Phycology 2001, 13(6):89-106.
71. Mcpeak R.H., Glanz D. Harvesting California's kelp forest. Oceanus 1984, 27(1):19-26.
72. Mitchell M.E., Guiry M.D. Carrageen: a local habitation or a name?. Journal of Ethnopharmacology 1983, 9:347-351.
73. Moen E., Horn S., Ostgaard K. Alginate degradation during anaerobic digestion of Laminaria hyperborea stipes. Journal of Applied Phycology 1997, 9:157-166.
74. Moirano A.L. Sulfated seaweed polysaccharides. Food Colloids 1977, 347-381. AVI Publishing Co., Westport, CT. H.D. Graham (Ed.).
75. Mørc Ý.A. Novel Alginate Microcapsules for Cell Therapy 2008, Ph. D. Thesis, Norwegian University of Science and Technology.
76. Murano E. Chemical structure and quality of agars from Gracilaria. Journal of Applied Phycology 1995, 7(3):245-254.
77. MurilloÁlvarez J.I., Hernández-Carmona G. Monomer composition and sequence of sodium alginate extracted at pilot plant scales from three commercially important seaweeds from México. Journal of Applied Phycology 2007, 19(5):545-548.
78. Myklestad S. Ion-exchange of Brown algae Determination of rate mechanism for calcium hydrogen ion exchange for particles from Laminaria hyperborea and Laminaria digitata. Journal of Applied Chemistry 1968, 18:30-36.
79. Nagaoka M., Shibata H., Kimura-Takagi I., Hashimoto S. Anti-ulcer effects and biological activities of polysaccharides from marine algae. BioFactors 2000, 12:267-274.
80. Neori A., Shpigel M., Ben-Ezra D. Sustainable integrated system for culture of fish seaweed and abalone. Aquaculture 2000, 186:279-291.
81. Nishigawa K. Extract method of alginic acid. Research Methods of Algae 1985, 624-626. K. Nishigawa, M. Chthara (Eds.).
82. Painter T.J. Algal polysaccharides. The Polysaccharides 1983, vol. 2:195-285. Academic Press, New York.
83. Pajic-Lijakovic I., Plavsic M., Nedovic V., Bugarski B. Investigation of Ca-alginate hydrogel rheological behavior in conjunction with immobilized yeast cell growth dynamics. Journal of Microencapsulation 2007, 24(5):420-429.
84. Penman A., Rees D.A. Carrageenans-Part IX: Methylation analysis of galactan sulphates from Furcellaria fastigiata, Gigartina canaliculata, Gigartina chamissoi, Gigartina atropurpurea, Ahnfeltia durvillaei, Gymnogongrus furcellatus, Eucheuma cottonii, Eucheuma spinosum, Eucheuma isiforme, Eucheuma uncinatum, Aghardiella tenera, Pachymenia hymantophora and Gloiopeltis cervicornis, Structure of ε-carrageenan. Journal of Chemical Society 1973, 2182-2187. Perkin Trans 1.
85. Pernas A.J., Smidsrød O., Larsen B., Haug A. Chemical heterogeneity of Carrageenans as shown by fractional precipitation with potassium chloride. Acta Chemica Scandinavica 1967, 21:98-110.
86. Rees D. Structure conformation, and mechanism in the formation of polysaccharide gels and networks. Adv Carbohyd Chem Biochem 1969, 24:267-332.
87. Reyes-Tisnado R., López-Gutiérrez F., Hernández-Carmona G., Vernon-Carter J., Castro-Moroyoqui P. Sodium and potassium alginates extracted from Macrocystis pyrifera algae for use in dental impression materials. Ciencias Marinas 2004, 30(3):189-199.
88. Rincones R.E., Yu S., Pedersén M. Effect of dark treatment on the starch degradation and the agar quality of cultivated Gracilaria lemaneiformis (Rhodophyta, Gracilariales) from Venezuela. Hydrobiologia 1993, 260(261):633-640.
89. Robledo D., Freile-Pelegrin Y. Managing the interactions between plants and animals in marine multi-trophic aquaculture: integrated shrimp and valuable low food chain organisms with seaweeds. All Flesh is Grass, Plant-Animal Interrelationships Series: Cellular Origin, Life in Extreme Habitats and Astrobiology 2011, vol. 16. Springer. 1st edition. Z. Dubinsky, J. Seckbach (Eds.).
90. Rodríguez-Montesinos Y.E., Arvizu-Higuera D.L., Hernández-Carmona G. Seasonal variation on size and chemical constituents of Sargassum sinicola Setchel et Gardner from Bahía de La Paz, Baja California Sur, México. Phycological Research 2008, 56(1):34-39.
91. Rodríguez-Montesinos Y.E., Hernández-Carmona G., Arvizu-Higuera D.L. Aprovechamiento de los líquidos residuales en la etapa de conversión de alginato de calcio en ácido algínico durante el proceso de producción de alginatos. Oceánides 2005, 20(1):1-7.
92. Schuldt U., Hunkeler D. Alginate-cellulose sulphate-oligocation microcapsules: Optimization of mass transport and mechanical properties. Journal of Microencapsulation 2007, 24(1):1-10.
93. Schweiger R.J., Guardado-Puentes J., Hernandez-Garibay E., Caloca-Quiñones C., Bautista-Alcantar J. Planta Piloto de Carragenanos 1992, Reporte interno. Centro Regional de Investigación Pesquera de Ensenada. INP.
94. Scott G.V. Increasing viscosity of carrageenan-containing composition with microwave radiation 1984, US Patent No. 4,478,818.
95. Smidsrod O., Draget K.I. Chemistry and physical properties of alginates. Carbohydrates in Europe 1996, 14:6-13.
96. Smith, Dband Cook W.H. Fractionation of carrageenin. Archives of Biochemistry and Biophysics 1953, 45:232-233.
97. Sousa A.M.M., Alves V.D., Morais S., Delerue-Matos C., Gonçalves M.P. Agar extraction from integrated multitrophic aquacultured Gracilaria vermiculophylla: evaluation of a microwave-assisted process using response surface methodology. Bioresource Technology 2010, 101:3258-3267.
98. Stancioff D.J. Selective extraction of hydrocolloid fractions from sea plants 1965, US Patent No. 3176003.
99. Stanley N. Production, properties and uses of carrageenan. Production and Utilization of Products from Commercial Seaweeds, FAO Fisheries Technical Papers 288, Rome.FAO 1987, 116-146. D.J. McHugh (Ed.).
100. Tang J.C., Taniguchi H., Chu H., Zhou Q., Nagata S. Isolation and characterization of alginate degrading bacteria for disposal of seaweeds waste. Letters in Applied Microbiology 2009, 48:38-43.
101. Tavares-Salgado L., Tomazetto R., Paes-Cinelli L., Farina M., Menezes-Amadofilho G. The influence of brown algae alginates on phenolic compounds capability of ultraviolet radiation absorption in vitro. Brazilian Journal of Oceanography 2007, 55(2):145-154.
102. Truus K., Vaher M., Taure I. Algal biomass from Fucus vesiculosus (Phaeophyta): investigation of the mineral and alginate components. Proceedings of the Estonian Academy of Sciences 2001, 50(2):95-103.
103. Tseng C.K. Phycocolloids: useful seaweed polysaccharides. Colloid Chemistry: Theoretical and Applied 1946, Vol. 6:629-734. Reinhold, New York.
104. Undp/Fao Training manual on Gracilaria culture and seaweed processing in China 1990, Training Manual 6. Regional sea-farming development and demonstration project (RAS/90/002)-UNDP/FAO, People's Republic of China, China.
105. Usov A.I. Structural analysis of red seaweed galactans of agar and carrageenan groups. Food Hydrocolloids 1998, 12:301-308.
106. Usov A.I., Arkhipova V.S. Polysaccharides of algae XXX. Methylation of κ-carrageenan type polysaccharides of the red seaweeds Tichocarpus crinitus (Gmel.) Rupr., Furcellaria fastigiata (Huds.) Lam. and Phyllophora nervosa (De Cand.) Grev. - Bioorganicheskaya Khimiya 1981, 7:385-390.
107. Usov A.I., Shashkov A.S. Polysaccharides of algae XXXIV: Detection of iota-carrageenan in Phyllophora brodiaei (Turn.) J. Ag. (Rhodophyta) using 13 C-NMR spectroscopy. Botanica Marina 1985, 28:367-373.
108. Usov A.I., Yarotsky S.V., Shashkov A. 13C NMR spectroscopy of red algal galactans. Biopolymers 1980, 19:977-990.
109. van de Velde F. Structure and function of hybrid carrageenans. Food Hydrocolloids 2008, 22:727-734.
110. van de Velde F., De Ruiter G.A. Carrageenan. Biopolymers V 6 Polysaccharides II. Polysaccharides from Eukaryotes 2002, 245-274. Wiley, Weinheim. E.J. Vandamme, S.D. Baets, A. Steinbèuchel (Eds.).
111. Vauchel P., Arhaliass A., Legrand J., Kaas R., Baron R. Decrease in dynamic viscosity and average molecular weight of alginate from Laminaria digitata during alkaline extraction. Journal of Phycology 2008, 44(2):515-517.
112. Vauchel P., Kaas R., Arhaliass A., Baron R., Legrand J. A new process for extracting alginates from Laminaria digitata: Reactive extrusion. Food and Bioprocess Technology 2008, 1(3):297-300.
113. Vauchel P., Leroux K., Kaas R., Arhaliass A., Baron R., Legrand J. Kinetics modeling of alginate alkaline extraction from Laminaria digitata. Bioresource Technology 2009, 100(3):1291-1296.
114. Warburton R.N. Patient safety-how much is enough?. Health Policy 2005, 71:223-232.
115. Williams P.A. Molecular interactions of plant and algal polysaccharides. Structural Chemistry 2009, 20(2):299-330.
116. Yabur R., Bashan Y., Hernández-Carmona G. Alginate from the macroalgae Sargassum sinicola as a novel source for microbial immobilization material in wastewater treatment and plant growth promotion. Journal of Applied Phycology 2007, 19(1):43-53.
117. Younis Y.M.H., Tecleab S., Gheza T., Rahama I.H. Determination of the biomass and phycocolloid contents of some marine algae from Eritrea. Journal KAU: Marine Science 2000, 11:19-25.
118. Yu S., Pedersén M. The effect of salinfity changes on the activity of α-galactosidase of the red algae Gracilaria tenuistipitata and G. sordida. Botanica Marina 1990, 33:385-391.