Development of a one-step PCR assay with nine primer pairs for the detection of five diarrheagenic escherichia coli types

Journal of Microbiology and Biotechnology

Volumn 24, Issue 6, 2014, Pages 862-868

  Oh, Kyung Hwan ^a   Kim, Soo Bok ^b   Park, Mi Sun ^b   Cho, Seung Hak ^a  

^a Korea National Institute of Health   (South Korea)

^b Kogene Biotech Co Ltd Geumcheon gu   (South Korea)

Author keywords

Diarrheagenic E. coli; Multiplex PCR; Sensitivity and specificity

Indexed keywords

BACTERIAL DNA; ESCHERICHIA COLI PROTEIN; PRIMER DNA; VIRULENCE FACTOR;

AGGR GENE; ARTICLE; BACILLUS CEREUS; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BACTERIUM ISOLATION; BFPA GENE; BIOASSAY; CAMPYLOBACTER JEJUNI; CLOSTRIDIUM PERFRINGENS; COLONY FORMING UNIT; CONTROLLED STUDY; DIARRHEAGENIC ESCHERICHIA COLI; DNA ISOLATION; DNA SEQUENCE; EAEA GENE; FECES ANALYSIS; HUMAN; INSTRUMENT VALIDATION; IPAH GENE; KOREA; LIMIT OF DETECTION; LISTERIA MONOCYTOGENES; LT GENE; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PROCESS DEVELOPMENT; SALMONELLA TYPHIMURIUM; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS AUREUS; STH GENE; STP GENE; STX1 GENE; STX2 GENE; VIBRIO PARAHAEMOLYTICUS; YERSINIA ENTEROCOLITICA; CLASSIFICATION; DIARRHEA; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; EVALUATION STUDY; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; POLYMERASE CHAIN REACTION; PROCEDURES;

DIARRHEA; DNA PRIMERS; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI PROTEINS; HUMANS; POLYMERASE CHAIN REACTION; VIRULENCE FACTORS;

EID: 84902590481     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.1312.12031     Document Type: Article

References (23)

1. Bernier C, Gounon P, Le Bouguenec C. 2002. Identification of an aggregative adhesion fimbria (AAF) type III-encoding operon in enteroaggregative Escherichia coli as a sensitive probe for detecting the AAF-encoding operon family. Infect. Immun. 70: 4302-4311.
2. Botteldoorn N, Heyndrickx M, Rijpens N, Herman L. 2003. Detection and characterization of verotoxigenic Escherichia coli by VTEC/EHEC multiplex PCR in porcine faeces and pig carcass swabs. Res. Microbiol. 154: 97-104.
3. Clarke SC. 2001. Diarrhoeagenic Escherichia coli: an emerging problem? Diagn. Microbiol. Infect. Dis. 41: 93-99.
4. Corpet F. 1988. Multiple sequence alignment with hierarchical clustering. Nucl. Acids Res. 16: 10881-10890.
5. Fujuoka M, Saito M, Otomo Y. 2008. Direct detection of diarrheagenic Escherichia coli in patient stool specimens by developed multiplex PCRs for the establishment of surveillance system of diarrheagenic Escherichia coli. Jpn. J. Med. Technol. 8: 1041-1046.
6. Fujioka M, Otomo Y, Ahsan CR. 2013. A novel single-step multiplex polymerase chain reaction assay for the detection of diarrheagenic Escherichia coli. J. Microbiol. Methods 92: 289- 292.
7. Gomez-Duarte OG, Bai J, Newel E. 2009. Detection of E. coli, Salmonella spp., Shigella spp., Yersinia entercolitica, Vibrio cholerae, and Campylobacter spp. enteropathogens by threereaction multplex PCR. Diagn. Microbiol. Infect. Dis. 63: 1-9.
8. Kaper JB, Nataro JP, Mobley HL. 2004. Pathogenic Escherichia coli. Nat. Rev. Microbiol. 2: 123-140.
9. Lang AL, Tsai YL, Mayer CL, Patton KC, Palmer CJ. 1994. Multiplex PCR for detection of the heat-labile toxin gene and Shiga-like toxin I and II genes in Escherichia coli isolated from natural waters. Appl. Environ. Microbiol. 60: 3145-3149.
10. Lasaro MA, Rodrigues JF, Mathias-Sanos C, Guth BE, Balan A, Sbrogio-Almeida ME, Ferreira LC. 2008. Genetic diversity of heat-labile toxin expressd by enterotoxigenic Escherichia coli strains isolated from humans. J. Bacteriol. 190: 2400-2410.
11. Nataro JP, Kaper JB. 1998. Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11: 142-201.
12. Nataro JP, Deng Y, Walker K. 1994. AggR, a transcriptional activator of aggregative adherence factor I expression. J. Bacteriol. 176: 4691-4699.
13. Osek J. 2003. Development of a multiplex PCR approach for the identification of Shiga toxin-producing Escherichia coli strains and their major virulence factor genes. J. Appl. Microbiol. 95: 1217-1225.
14. Pass MA, Odedra R, Batt RM. 2000. Multiplex PCR for identification of Escherichia coli virulence genes. J. Clin. Microbiol. 38: 2001-2004.
15. Rappelli P, Maddau G, Mannu F, Colombo MM, Fiori PL, Cappuccinelli P. 2001. Development of a set of multiplex PCR assays for the simultaneous identification of enterotoxigenic, enteropathogenic, enterohemorrhagic and enteroinvasive Escherichia coli. Microbiologica 24: 77-83.
16. Savarino SJ, Fasano A, Watson J, Martin BM, Levine MM, Guandalini S, Guerry P. 1993. Enteroaggregative Escherichia coli heat-stable enterotoxin 1 represents another family of E. coli heat-stable toxin. Proc. Natl. Acad. Sci. USA 90: 3093- 3097.
17. Sethabutr O, Venkatesan M, Yam S, Pang LW, Smoak BL, Sang WK, et al. 2000. Detection of PCR products of the ipaH gene from Shigella and enteroinvasive Escherichia coli by enzyme linked immunosorbent assay. Diagn. Microbiol. Infect. Dis. 37: 11-16.
18. Sheikh J, Czezulin JR, Harrington S, Hicks SI, Henderson R, Bouguenec Cle, et al. 2002. A novel dispersin protein in enteroaggregative Escherichia coli. J. Clin. Investig. 110: 1329- 1337.
19. Sjöling A.;, Wiklund G, Savarino SJ, Cohen DI, Svennerholm AM. 2007. Comparative analyses of phenotypic and genotypic methods for detection of enterotoxigenic Escherichia coli toxins and colonization factors. J. Clin. Microbiol. 45: 3295- 3301.
20. Tarr PI, Gordon CA, Handler CWL. 2005. Shiga-toxinproducing Escherichia coli and haemolytic uraemic syndrome. Lancet 365: 1073-1086.
21. Tobias J, Vutukuru SR. 2012. Simple and rapid multiplex PCR for identification of the main human diarrheagenic Escherichia coli. Microbiol. Res. 167: 564-570.
22. Trabulsi LR, Keller R, Gomes TAT. 2002. Typical and atypical enteropathogenic Escherichia coli. Emerg. Infect. Dis. 8: 508-513.
23. Watterworth L, Topp E, Schraft H, Leung KT. 2005. Multiplex PCR-DNA probe assay for the detection of pathogenic Escherichia coli. J. Microbiol. Methods 60: 93-105.