Simple and rapid multiplex PCR for identification of the main human diarrheagenic Escherichia coli

Microbiological Research

Volumn 167, Issue 9, 2012, Pages 564-570

  Tobias, Joshua ^a   Vutukuru, Sreekanth Reddy ^a  

^a UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

Diarrheagenic E. coli; Multiplex PCR

Indexed keywords

E. COLI; ENTEROAGGREGATIVE; ENTEROTOXIGENIC; MULTIPLEX PCR;

MULTIPLEXING;

ESCHERICHIA COLI;

ESCHERICHIA COLI PROTEIN;

CLASSIFICATION; COLIFORM BACTERIUM; DIARRHEAL DISEASE; FECES; IDENTIFICATION METHOD; POLYMERASE CHAIN REACTION; PUBLIC HEALTH; YOUNG POPULATION;

ARTICLE; BACTERIUM IDENTIFICATION; CLASSIFICATION; DIARRHEA; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTION; EVALUATION; FEMALE; GENETICS; HUMAN; INFANT; ISOLATION AND PURIFICATION; MALE; METHODOLOGY; MICROBIOLOGY; MULTIPLEX POLYMERASE CHAIN REACTION;

BACTERIAL TYPING TECHNIQUES; DIARRHEA; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI PROTEINS; FEMALE; HUMANS; INFANT; MALE; MULTIPLEX POLYMERASE CHAIN REACTION;

ESCHERICHIA COLI;

EID: 84867331498     PISSN: 09445013     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.micres.2011.11.006     Document Type: Article

References (26)

1. Aranda K.R., Fabbricotti S.H., Fagundes-Neto U., Scaletsky I.C. Single multiplex assay to identify simultaneously enteropathogenic, enteroaggregative, enterotoxigenic, enteroinvasive and Shiga toxin-producing Escherichia coli strains in Brazilian children. FEMS Microbiol Lett 2007, 267:145-150.
2. Brandal L.T., Lindstedt B.A., Aas L., Stavnes T.L., Lassen J., Kapperud G. Octaplex PCR and fluorescence-based capillary electrophoresis for identification of human diarrheagenic Escherichia coli and Shigella spp. J Microbiol Methods 2007, 68:331-341.
3. Bueris V., Sircili M.P., Taddei C.R., Fernandes dos Santos M., Franzolin M.R., Martinez M.B., et al. Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil. Mem Inst Oswaldo Cruz (Rio de Janeiro) 2007, 102:839-844.
4. Cennimo D.J., Koo H., Mohamed J.A., Huang D.B., Chiang T. Enteroaggregative Escherichia coli: a review of trends, diagnosis, and treatment. Infect Med 2007, (March):100-110.
5. Fujioka M., Kasai K., Miura T., Sato T., Otomo Y. Rapid diagnostic method for the detection of diarrheagenic Escherichia coli by multiplex PCR. Jpn J Infect Dis 2009, 62:476-480.
6. Hardegen C., Messler S., Henrich B., Pfeffer K., Würthner J., MacKenzie C.R. A set of novel multiplex Taqman real-time PCRs for the detection of diarrhoeagenic Escherichia coli and its use in determining the prevalence of EPEC and EAEC in a university hospital. Ann Clin Microbiol Antimicrob 2010, 9:5.
7. Hien B.T.T., Scheutz F., Cam P.D., Serichantalergs O., Huong T.T., Thu T.M., et al. Diarrheagenic Escherichia coli and shigella strains isolated from children in a hospital case-control study in Hanoi, Vietnam. J Clin Microbiol 2008, 46:996-1004.
8. Huang D.B., Mohanty A., DuPont H.L., Okhuysen P.C., Chiang T. A review of an emerging enteric pathogen: enteroaggregative Escherichia coli. J Med Microbiol 2006, 55(October (Pt 10)):1303-1311. Review. Erratum in: J Med Microbiol 2007;56(Pt 1):142.
9. Jerse A.E., Martin W.C., Galen J.E., Kaper J.B. Oligonucleotide probe for detection of the enteropathogenic Escherichia coli (EPEC) adherence factor of localized adherent EPEC. J Clin Microbiol 1990, 28:2842-2844.
10. Kaper J.B. Defining EPEC. Rev Microbiol (Sao Paulo) 1996, 27:130-133.
11. Khairun N., Dilruba A., Johirul I., Lutful Kabir F.M., Hossain M.A. Usefulness of a multiplex PCR for detection of diarrheagenic Escherichia coli in a diagnostic microbiology laboratory setting. Bangladesh J Med Microbiol 2007, 01:38-42.
12. Kimata K., Shima T., Shimizu M., Tanaka D., Isobe J., Gyobu Y., et al. Rapid categorization of pathogenic Escherichia coli by multiplex PCR. Microbiol Immunol 2005, 49:485-492.
13. Nataro J.P., Kaper J.B. Diarrheagenic Escherichia coli. Clin Microbiol 1998, 11:142-201.
14. Nessa K., Ahmed D., Islam J., Kabir F.L., Hossain M.A. Usefulness of a multiplex PCR for detection of diarrheagenic Escherichia coli in a diagnostic microbiology laboratory setting. Bangladesh J Med Microbiol 2007, 1:38-42.
15. Nguyen T.V., Le Van P., Le Huy C., Gia K.N., Weintraub A. Detection and characterization of diarrheagenic Escherichia coli from young children in Hanoi. Vietnam J Clin Microbiol 2005, 43:755-760.
16. Okeke I.N. Diarrheagenic Escherichia coli in sub-Saharan Africa: status, uncertainties and necessities. J Infect Dev Ctries 2009, 3:817-842.
17. Parashar U., Umesh D., Bresee J.S., Joseph S., Glass R.I., Roger I. The global burden of diarrhoeal disease in children. Bull WHO 2003, 81:236.
18. Porat N., Levy A., Fraser D., Deckelbaum R.J., Dagan R. Prevalence of intestinal infections caused by diarrheagenic Escherichia coli in Bedouin infants and young children in Southern Israel. Pediatr Infect Dis J 1998, 17:482-488.
19. Qadri E., Svennerholm A.M., Faruque As, Sack R.B. Enterotoxigenic Escherichia coli in developing countries: epidemiology, microbiology, clinical features, treatment, and prevention. Clin Microbiol Rev 2005, 18:465-483.
20. Rajendrana P., Rao Ajjampura S.S., Chidambarama D., Chandrabosea G., Thangarajb B., Sarkarb R., et al. Pathotypes of diarrheagenic Escherichia coli in children attending a tertiary care hospital in South India. Diagn Microbiol Infect Dis 2010, 68:117-122.
21. Reyes D., Vilchez S., Paniagua M., Colque-Navarro P., Weintraub A., Möllby R., et al. Diarrheagenic Escherichia coli markers and phenotypes among fecal E. coli isolates collected from Nicaraguan infants. J Clin Microbiol 2010, 48:3395-3396.
22. Rodas C., Iniguez V., Qadri F., Wiklund G., Svennerholm A.-M., Sjöling å. Development of multiplex PCR assays for detection of enterotoxigenic Escherichia coli colonization factors and toxins. J Clin Microbiol 2009, 47:1218-1220.
23. Toma C., Lu Y., Higa N., Nakasone N., Chinen I., Baschkier A., et al. Multiplex PCR assay for identification of human diarrheagenic Escherichia coli. J Clin Microbiol 2003, 42:2669-2671.
24. Trabulsi L.R., Keller R., Tardelli Gomes T.A. Typical and atypical enteropathogenic Escherichia coli. Emerg Infect Dis 2002, 8:508-513.
25. Vidal P., Vidal M., Lagos R., Levine M., Prado V. Multiplex PCR for diagnosis of enteric infections associated with diarrheagenic Escherichia coli. J Clin Microbiol 2004, 42:1787-1789.
26. Vilchez S., Reyes D., Paniagua M., Bucardo F., Möllby R., Weintraub A. Prevalence of diarrhoeagenic Escherichia coli in children from Leon, Nicaragua. J Med Microbiol 2009, 58:630-637.