Development of a set of multiplex PCR assays for the simultaneous identificatton of enterotoxigenic, enteropathogenic, enterohemorrhagic and enteroinvasive Escherichia coli

New Microbiologica

Volumn 24, Issue 1, 2001, Pages 77-83

  Rappelli, P ^a   Maddau, G ^a   Mannu, F ^b   Colombo, M M ^c   Fiori, P L ^a   Cappuccinelli, P ^a  

^a UNIVERSITY OF SASSARI   (Italy)

^b University La Sapienza ^*  (Italy)

^c SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; PRIMER DNA;

ANGOLA; ARTICLE; CHEMISTRY; CHILD; CLASSIFICATION; COMPARATIVE STUDY; DIARRHEA; ENTEROBACTER INFECTION; ESCHERICHIA COLI; FECES; GENETICS; HUMAN; ISOLATION AND PURIFICATION; ITALY; METHODOLOGY; MICROBIOLOGY; MOZAMBIQUE; PATHOGENICITY; POLYMERASE CHAIN REACTION; VIRULENCE;

ANGOLA; CHILD; DIARRHEA; DNA PRIMERS; DNA, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; FECES; HUMANS; ITALY; MOZAMBIQUE; POLYMERASE CHAIN REACTION; VIRULENCE;

EID: 0035228492     PISSN: 11217138     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. CALUNDUNGO, R., MADDAU, G., RAPPELLI, P., CAPPUCCINELLI, P., LEITE, F., FRANCISCO, M., and COLOMBO, M. (1994). Escherichia cou and enteritis in Angola. The Lancet, 344, 538-539.
2. DONNENBERG, M.S., GIRON, J.A., NATARO, J.P., and KAPER, J.B. (1992). A plasmid-encoded type IV fimbrial gene of enteropathogenic Escherichia coli associated with localized adherence. Molecular Microbiology, 6, 3427-3437.
3. FAGAN, P.K., HORNITZKY, M.A., BETTELHEIMM K.A., and DJORDJEVIC, S.P. (1999) Detection of Shiga-Like toxin (stx1 and stx2), intimin (eaeA), and enterohemorrhagic Escherichia coli (EHEC) hemolisin (EHEC hlyA) genes in animal feces by multiplex PCR. Applied and Environmental Microbiology, 65, 868-872.
4. FRANKEL, G., RILEY, L., GIRON, J.A., VALMASSOI, J., FRIEDMANN, A., STROCKBINE, N., FALKOW, S., and SCHOOLNIK, G.K. (1990). Detection of Shigella in feces using DNA amplification. Journal of Infectious Diseases, 161, 1252-1256.
5. GIRON, J.A., DONNENBERG, M.S., MARTIN, W.C., JARVIS, K.G., and KAPER , J.B. (1991). Diffuse-adhering Escherichia coli (DAEC) as a putative cause of diarrhea in Mayan children in Mexico. Journal of Infectious Diseases, 163, 507-513.
6. JERSE, A.E., YU, J., TALL, B.D., and KAPER, J.B. (1990). A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proceedings of the National Academy of Sciences USA, 87, 7839-7843.
7. KWOK, S., and HIGUCHI, R. (1989). Avoiding false positives with PCR. Nature (London), 339, 237-238.
8. LEVINE, M.M. (1987). Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. Journal of Infectious Diseases, 155, 377-389.
9. MCDANIELS, A.E., RICE, E.W., REYES, A.L., JOHNSON, C.H., HAUGLAND, R.A., and STELMA, G.N.JR. (1996). Confirmational identification of Escherichia coli, a comparison of genotypic and phenotypic assays for glutamate decarboxylase and beta-D-glucuronidase. Applied and Environmental Microbiology, 62, 3350-3354.
10. MOSELEY, S.L., ECHERVERRIAM P., SERIWATANA, J. et al. (1982). Identification of enterotoxigenic Escherichia coli by colony hybridization using three enterotoxin gene probes. Journal of Infectious Diseases, 145, 863-869.
11. NATARO, J.P., and KAPER, J.B. (1998). Diarrheagenic Escherichia coli. Clinical Microbiology Reviews, 11, 142-201.
12. O'BRIEN, A.D., and HOLMES, R.K. (1987). Shiga and Shiga like toxins. Microbiological Reviews, 51, 206-220.
13. OLIVE, D.M. (1989). Detection of enterotoxigenic Escherichia coli after polymerase chain reaction amplification with a thermostable DNA polymerase. Journal of Clinical Microbiology, 27, 261-265.
14. ORJAN, O., and STROCKBINE, N. (1993). PCR detection of heat-stable, heat-labile, and Shiga toxin genes in Escherichia coli. In: Persing, D.H., Smith, T.F., Tenover, F.C., and White, T.J. Eds, Diagnostic molecular microbiology. Principles and applications. Washington, D.C., American Society for Microbiology, 271-276.
15. POLLARD, D.R., JOHNSON, W.M., LIOR, H., TYLER, S.D., and ROZEE, K.R. (1990). Rapid and specific detection of verotoxin genes in Escherichia coli by polymerase chain reaction. Journal of Clinical Microbiology, 28, 540-545.
16. SASAKAWA, C., BUYSSE, J.M., and WATANABE, H. (1992). The large virulence plasmid of Shigella. Current Topics in Microbiology and Immunology, 180, 21-44.
17. SEARS, C.L., and KAPER, J.B. (1996). Enteric bacterial toxins: mechanisms of action and linkage to intestinal secretion. Microbiological Reviews, 60, 167-215.
18. STACY-PHIPPS, S., MECCA, J.J., and WEISS, J.B. (1995). Multiplex PCR assay and simple preparation method for stool specimens detect enterotoxigenic Escherichia coli DNA during course of infection. Journal of Clinical Microbiology, 33, 1054-1059.
19. SUNABE, T., and HOMNA, Y. (1998). Relationship between O-serogoup and presence of pathogenic factor genes in Escherichia coli. Microbiology and Immunology 42, 845-849.
20. VIAL, P.A., ROBINS BROWNE, R., LIOR, H. et al. (1988). Characterization of enteroadherent-aggregative Escherichia coli, a putative agent of diarrheal disease. Journal of Infectious Diseases, 158, 70-79.
21. YU, J., and KAPER, J.B. (1992). Cloning and characterization of the eae gene of enterohaemorrhagic Escherichia coli O157:H7. Molecular Microbiology, 6, 411-417.