GTS-21, an α7-nicotinic acetylcholine receptor agonist, modulates Th1 differentiation in CD4+ T cells from patients with rheumatoid arthritis

Experimental and Therapeutic Medicine

Volumn 8, Issue 2, 2014, Pages 557-562

  Wu, Shiyao ^a   Zhao, Hongjun ^a   Luo, Hui ^a   Xiao, Xianzhong ^b   Zhang, Huali ^b   Li, Tong ^a   Zuo, Xiaoxia ^a  

^a CENTRAL SOUTH UNIVERSITY   (China)

^b Central South University   (China)

Author keywords

GTS 21; Rheumatoid arthritis; TBX21; Th1 cells; 7nAchR

Indexed keywords

3 (2, 4 DIMETHOXYBENZYLIDENE) ANABASEINE; BUNGAROTOXIN RECEPTOR; C REACTIVE PROTEIN; CYCLIC CITRULLINATED PEPTIDE ANTIBODY; GAMMA INTERFERON; RHEUMATOID FACTOR; T BOX TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR T BET; UNCLASSIFIED DRUG;

ADULT; ARTICLE; BONE INJURY; CD4+ T LYMPHOCYTE; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL VIABILITY; CLINICAL ARTICLE; CONTROLLED STUDY; DAS28; DISEASE ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; ERYTHROCYTE SEDIMENTATION RATE; FEMALE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MALE; PROTEIN EXPRESSION; RHEUMATOID ARTHRITIS; SYNOVITIS; TH1 CELL; VISUAL ANALOG SCALE; WESTERN BLOTTING;

EID: 84902574015     PISSN: 17920981     EISSN: 17921015     Source Type: Journal    
DOI: 10.3892/etm.2014.1754     Document Type: Article

References (25)

1. Cope AP, Schulze-Koops H and Aringer M: The central role of T cells in rheumatoid arthritis. Clin Exp Rheumatol 25 (5 Suppl 46): S4-S11, 2007.
2. Yamada H, Nakashima Y, Okazaki K, et al: Th1 but not Th17 cells predominate in the joints of patients with rheumatoid arthritis. Ann Rheum Dis 67: 1299-1304, 2008.
3. Cañete JD, Martinez SE, Farrés J, et al: Differential Th1/Th2 cytokine patterns in chronic arthritis: interferon gamma is highly expressed in synovium of rheumatoid arthritis compared with seronegative spondyloarthropathies. Ann Rheum Dis 59: 263-268, 2000.
4. Nissinen R, Leirisalo-Repo M, Tiittanen M, et al: CCR3, CCR5, interleukin 4, and interferon-gamma expression on synovial and peripheral T cells and monocytes in patients with rheumatoid arthritis. J Rheumatol 30: 1928-1934, 2003.
5. Szabo SJ, Sullivan BM, Stemmann C, Satoskar AR, Sleckman BP and Glimcher LH: Distinct effects of T-bet in TH1 lineage commitment and IFN-gamma production in CD4 and CD8 T cells. Science 295: 338-342, 2002.
6. + T cells to Th1 cells requires MAP kinase JNK2. Immunity 9: 575-585, 1998.
7. Pavlov VA, Ochani M, Yang LH, et al: Selective alpha7-nicotinic acetylcholine receptor agonist GTS-21 improves survival in murine endotoxemia and severe sepsis. Crit Care Med 35: 1139-1144, 2007.
8. Wang H, Liao H, Ochani M, et al: Cholinergic agonists inhibit HMGB1 release and improve survival in experimental sepsis. Nat Med 10: 1216-1221, 2004.
9. Wang H, Yu M, Ochani M, et al: Nicotinic acetylcholine receptor alpha7 subunit is an essential regulator of inflammation. Nature 421: 384-388, 2003.
10. Bruchfeld A, Goldstein RS, Chavan S, et al: Whole blood cytokine attenuation by cholinergic agonists ex vivo and relationship to vagus nerve activity in rheumatoid arthritis. J Intern Med 268: 94-101, 2010.
11. de Jonge WJ and Ulloa L: The alpha7 nicotinic acetylcholine receptor as a pharmacological target for inflammation. Br J Pharmacol 151: 915-929, 2007.
12. Kitagawa H, Takenouchi T, Azuma R, et al: Safety, pharmacokinetics, and effects on cognitive function of multiple doses of GTS-21 in healthy, male volunteers. Neuropsychopharmacol 28: 542-551, 2003.
13. Olincy A, Harris JG, Johnson LL, et al: Proof-of-concept trial of an alpha7 nicotinic agonist in schizophrenia. Arch Gen Psychiatry 63: 630-638, 2006.
14. Conti-Fine BM, Navaneetham D, Lei S and Maus AD: Neuronal nicotinic receptors in non-neuronal cells: new mediators of tobacco toxicity? Eur J Pharmacol 393: 279-294, 2000.
15. Ahmed SA, Gogal RJ Jr and Walsh JE: A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H]thymidine incorporation assay. J Immunol Methods 170: 211-224, 1994.
16. O'Brien J, Wilson I, Orton T and Pognan F: Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem 267: 5421-5426, 2000.
17. Petersen CM, Christensen EI, Andresen BS and Møller BK: Internalization, lysosomal degradation and new synthesis of surface membrane CD4 in phorbol ester-activated T-lymphocytes and U-937 cells. Exp Cell Res 201: 160-173, 1992.
18. Mascher B, Schlenke P and Seyfarth M: Expression and kinetics of cytokines determined by intracellular staining using flow cytometry. J Immunol Methods 223: 115-121, 1999.
19. Kox M, van Velzen JF, Pompe JC, Hoedemaekers CW, van der Hoeven JG and Pickkers P: GTS-21 inhibits pro-inflammatory cytokine release independent of the Toll-like receptor stimulated via a transcriptional mechanism involving JAK2 activation. Biochem Pharmacol 78: 863-872, 2009.
20. Nizri E, Hamra-Amitay Y, Sicsic C, Lavon I and Brenner T: Anti-inflammatory properties of cholinergic up-regulation: A new role for acetylcholinesterase inhibitors. Neuropharmacology 50: 540-547, 2006.
21. Sato KZ, Fujii T, Watanabe Y, et al: Diversity of mRNA expression for muscarinic acetylcholine receptor subtypes and neuronal nicotinic acetylcholine receptor subunits in human mononuclear leukocytes and leukemic cell lines. Neurosci Lett 266: 17-20, 1999.
22. McGrath J, McDonald JW and Macdonald JK: Transdermal nicotine for induction of remission in ulcerative colitis. Cochrane Database Syst Rev 18: CD004722, 2004.
23. van Westerloo DJ, Giebelen IA, Florquin S, et al: The cholinergic anti-inflammatory pathway regulates the host response during septic peritonitis. J Infect Dis 191: 2138-2148, 2005.
24. van Maanen MA, Lebre MC, van der Poll T, et al: Stimulation of nicotinic acetylcholine receptors attenuates collagen-induced arthritis in mice. Arthritis Rheum 60: 114-122, 2009.
25. Li T, Zuo X, Zhou Y, et al: The vagus nerve and nicotinic receptors involve inhibition of HMGB1 release and early pro-inflammatory cytokines function in collagen-induced arthritis. J Clin Immunol 30: 213-220, 2010.