Engineering the lactococcal mevalonate pathway for increased sesquiterpene production

FEMS Microbiology Letters

Volumn 355, Issue 2, 2014, Pages 177-184

  Song, Adelene A ^a   Ong Abdullah, Janna ^a   Abdullah, Mohd P ^a   Shafee, Norazizah ^a   Othman, Roohaida ^b   Noor, Normah Mohd ^b   Rahim, Raha A ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

^b UNIVERSITI KEBANGSAAN MALAYSIA   (Malaysia)

Author keywords

Isoprenoid; Lactococcus lactis; Metabolic engineering; Mevalonate pathway; RT qPCR; Sesquiterpene

Indexed keywords

3 HYDROXY 3 METHYLGLUTARYL COENZYME A; ACETYL COENZYME A ACETYLTRANSFERASE; BACTERIAL PROTEIN; BETA SESQUIPHELLANDRENE; DIMETHYLALLYLDIPHOSPHATE; DIMETHYLALLYLTRANSFERASE; DIPHOSPHOMEVALONATE; DIPHOSPHOMEVALONATE DECARBOXYLASE; FARNESYL DIPHOSPHATE; GERANYL PYROPHOSPHATE; GERANYLGERANYL PYROPHOSPHATE; GERANYLGERANYLPYROPHOSPHATE SYNTHASE; GERANYLPYROPHOSPHATE SYNTHASE; HYDROXYMETHYLGLUTARYL COENZYME A SYNTHASE; ISOPENTENYL DIPHOSPHATE; MEVALONATE KINASE; MEVALONIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; PHOSPHOMEVALONIC ACID; PROTEIN MVAA; PROTEIN PMSTS; PROTEIN VMPSTS; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SESQUITERPENE DERIVATIVE; SESQUITERPENE SYNTHASE; UNCLASSIFIED DRUG; 2-C-METHYLERYTHRITOL 4-PHOSPHATE; BACTERIAL RNA; CULTURE MEDIUM; ERYTHRITOL; SESQUITERPENE; SUGAR PHOSPHATE;

ARTICLE; BACTERIAL GENE; BIOSYNTHESIS; CATALYSIS; CONTROLLED STUDY; DECARBOXYLATION; DOWN REGULATION; FNI GENE; GENE LOCATION; GENE OVEREXPRESSION; GENE TARGETING; GENETIC TRANSCRIPTION; HMCM GENE; ISPA GENE; LACTOCOCCUS LACTIS; METABOLIC ENGINEERING; MOLECULAR CLONING; MVAA GENE; MVAD GENE; MVK GENE; NONHUMAN; OPERON; PLASMID; PMK GENE; PMSTS GENE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; THIL GENE; TRANSCRIPTOMICS; UPREGULATION; VMPSTS GENE; ANALOGS AND DERIVATIVES; CHEMISTRY; CULTURE MEDIUM; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETIC ENGINEERING; GENETICS; MASS FRAGMENTOGRAPHY; METABOLISM; PROCEDURES;

ESCHERICHIA COLI; EUKARYOTA; LACTOCOCCUS LACTIS; POSIBACTERIA; PROKARYOTA;

CLONING, MOLECULAR; CULTURE MEDIA; ERYTHRITOL; ESCHERICHIA COLI; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; LACTOCOCCUS LACTIS; MEVALONIC ACID; PLASMIDS; RNA, BACTERIAL; SESQUITERPENES; SUGAR PHOSPHATES;

EID: 84902546289     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/1574-6968.12469     Document Type: Article

References (19)

1. Balibar CJ, Shen X & Tao J (2009) The mevalonate pathway of Staphylococcus aureus. J Bacteriol 191: 851-861.
2. Bustin SA, Benes V, Garson JA et al. (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55: 611-622.
3. Carlsen S, Ajikumar PK, Formenti LR, Zhou K, Too HP, Nielsen ML, Lantz AE, Kielland-Brandt MC & Stephanopoulos G (2013) Heterologous expression and characterization of bacterial 2-C-methyl-D-eryhtritol-4-phosphate pathway in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 97: 5753-5769.
4. Dimster-Denk D, Thorsness MK & Rine J (1994) Feedback regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in Saccharomyces cerevisiae. Mol Biol Cell 5: 655-665.
5. Engels B, Dahm P & Jennewein S (2008) Metabolic engineering of taxadiene biosynthesis in yeasts as a first step towards Taxol (Paclitaxel) production. Metab Eng 10: 201-206.
6. Hernandez I, Molenaar D, Beekwilder J, Bouwmeester H & van Hylckama Vlieg JET (2007) Expression of plant flavor genes in Lactococcus lactis. Appl Environ Microbiol 73: 1544-1552.
7. Kanehisa M & Goto S (2000) KEGG: kyoto encyclopedia of genes and genomes. Nucleic Acids Res 28: 27-30.
8. Kirby J & Keasling JD (2008) Metabolic engineering of microorganisms for isoprenoid production. Nat Prod Rep 25: 656-661.
9. Kuipers OP, de Ruyter PG, Kleerebezem M & de Vos WM (1998) Quorum sensing-controlled gene expression in lactic acid bacteria. J Biotechnol 64: 15-21.
10. Martin VJJ, Yasuo Y & Keasling JD (2001) The in vivo synthesis of plant sesquiterpenes by Escherichia coli. Biotechnol Bioeng 75: 497-503.
11. Martin VJ, Pitera DJ, Withers ST, Newman JD & Keasling JD (2003) Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol 21: 796-802.
12. Pfaffl MW (2001) A new mathematical model for relative quantification in real time RT-PCR. Nucleic Acids Res 29: E45.
13. Pichersky E & Gershenzon J (2002) The formation and function of plant volatiles: perfumes for pollinator attraction and defense. Curr Opin Plant Biol 5: 237-243.
14. Rebrikov DV & Trofimov DY (2006) Real-time PCR: a review of approaches to data analysis. Appl Biochem Microbiol 42: 455-463.
15. Rodríguez-Concepción M & Boronat A (2002) Elucidation of the methylerythritol phosphate pathway for isoprenoid biosynthesis in bacteria and plastids. A metabolic milestone achieved through genomics. Plant Physiol 130: 1079-1089.
16. Song AA, Abdullah JO, Abdullah MP, Shafee N & Rahim RA (2012a) Functional expression of an orchid fragrance gene in Lactococcus lactis. Int J Mol Sci 13: 1582-1597.
17. Song AA, Abdullah JO, Abdullah MP, Shafee N, Othman RO, Tan EF, Noor NM & Raha AR (2012b) Overexpressing 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) in the lactococcal mevalonate pathway for heterologous plant sesquiterpene production. PLoS One 7: e52444.
18. Tusruta H, Paddon CJ, Eng D, Lenihan JR, Horning T, Anthony LC, Regentin R, Keasling JD, Renninger NS & Newman JD (2009) High-level production of amorpha-4, 11-diene, a precursor of the antimalarial agent artemisinin in Escherichia coli. PLoS One 4: e4489.
19. Vandesompele J, Preter K, Pattyn F, Poppe B, Roy N, Paepe A & Speleman F (2002) Accurate normalization of real-time RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3: 1-12.