Manipulating gene expression and signaling activity in cultured mouse limb bud cells

Developmental Dynamics

Volumn 243, Issue 7, 2014, Pages 928-936

  Lewandowski, Jordan P ^a   Pursell, Taylor A ^a   Rabinowitz, Adam H ^a   Vokes, Steven A ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

Electroporation; Gene expression; Hedgehog; Limb bud culture; SiRNA

Indexed keywords

SMALL INTERFERING RNA; SONIC HEDGEHOG PROTEIN;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL POPULATION; CONTROLLED STUDY; ELECTROPORATION; GENE EXPRESSION; GENE EXPRESSION REGULATION; INTRACELLULAR SIGNALING; LIMB BUD; MOUSE; NONHUMAN; PLASMID; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UPREGULATION; ANIMAL; CYTOLOGY; GENETICS; METABOLISM; PHYSIOLOGY; SIGNAL TRANSDUCTION;

ERINACEIDAE; VERTEBRATA;

ANIMALS; CELLS, CULTURED; ELECTROPORATION; GENE EXPRESSION REGULATION, DEVELOPMENTAL; HEDGEHOG PROTEINS; LIMB BUDS; MICE; RNA, SMALL INTERFERING; SIGNAL TRANSDUCTION;

EID: 84902545767     PISSN: 10588388     EISSN: 10970177     Source Type: Journal    
DOI: 10.1002/dvdy.24128     Document Type: Article

References (36)

1. Akiyama H, Chaboissier MC, Martin JF, Schedl A, de Crombrugghe B. 2002. The transcription factor Sox9 has essential roles in successive steps of the chondrocyte differentiation pathway and is required for expression of Sox5 and Sox6. Genes Dev16:2813-2828.
2. Anderson R, Landry M, Muneoka K. 1993. Maintenance of ZPA signaling in cultured mouse limb bud cells. Development117:1421-1433.
3. Bastida MF, Sheth R, Ros MA. 2009. A BMP-Shh negative-feedback loop restricts Shh expression during limb development. Development136:3779-3789.
4. Benazet JD, Bischofberger M, Tiecke E, Goncalves A, Martin JF, Zuniga A, Naef F, Zeller R. 2009. A self-regulatory system of interlinked signaling feedback loops controls mouse limb patterning. Science323:1050-1053.
5. Bi W, Deng JM, Zhang Z, Behringer RR, de Crombrugghe B. 1999. Sox9 is required for cartilage formation. Nat Genet22:85-89.
6. Chiang C, Litingtung Y, Harris MP, Simandl BK, Li Y, Beachy PA, Fallon JF. 2001. Manifestation of the limb prepattern: limb development in the absence of sonic hedgehog function. Dev Biol236:421-435.
7. Cooper KL, Hu JK, ten Berge D, Fernandez-Teran M, Ros MA, Tabin CJ. 2011. Initiation of proximal-distal patterning in the vertebrate limb by signals and growth. Science332:1083-1086.
8. Denker AE, Nicoll SB, Tuan RS. 1995. Formation of cartilage-like spheroids by micromass cultures of murine C3H10T1/2 cells upon treatment with transforming growth factor-beta 1. Differentiation59:25-34.
9. Dessaud E, Yang LL, Hill K, Cox B, Ulloa F, Ribeiro A, Mynett A, Novitch BG, Briscoe J. 2007. Interpretation of the sonic hedgehog morphogen gradient by a temporal adaptation mechanism. Nature450:717-720.
10. Honig L, S. 1983. Polarizing activity of the avian limb examined on a cellular basis. New York: A.R. Liss.
11. Kawakami Y, Rodriguez-Leon J, Koth CM, Buscher D, Itoh T, Raya A, Ng JK, Esteban CR, Takahashi S, Henrique D, Schwarz MF, Asahara H, Izpisua Belmonte JC. 2003. MKP3 mediates the cellular response to FGF8 signalling in the vertebrate limb. Nat Cell Biol5:513-519.
12. Kawakami Y, Tsuda M, Takahashi S, Taniguchi N, Esteban CR, Zemmyo M, Furumatsu T, Lotz M, Izpisua Belmonte JC, Asahara H. 2005. Transcriptional coactivator PGC-1alpha regulates chondrogenesis via association with Sox9. Proc Natl Acad Sci USA102:2414-2419.
13. Laufer E, Nelson CE, Johnson RL, Morgan BA, Tabin C. 1994. Sonic hedgehog and Fgf-4 act through a signaling cascade and feedback loop to integrate growth and patterning of the developing limb bud. Cell79:993-1003.
14. Litingtung Y, Dahn RD, Li Y, Fallon JF, Chiang C. 2002. Shh and Gli3 are dispensable for limb skeleton formation but regulate digit number and identity. Nature418:979-983.
15. Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods25:402-408.
16. Long F, Chung UI, Ohba S, McMahon J, Kronenberg HM, McMahon AP. 2004. Ihh signaling is directly required for the osteoblast lineage in the endochondral skeleton. Development131:1309-1318.
17. Lopez-Rios J, Speziale D, Robay D, Scotti M, Osterwalder M, Nusspaumer G, Galli A, Hollander GA, Kmita M, Zeller R. 2012. GLI3 constrains digit number by controlling both progenitor proliferation and BMP-dependent exit to chondrogenesis. Dev Cell22:837-848.
18. Mariani FV, Ahn CP, Martin GR. 2008. Genetic evidence that FGFs have an instructive role in limb proximal-distal patterning. Nature453:401-405.
19. Marigo V, Scott MP, Johnson RL, Goodrich LV, Tabin CJ. 1996. Conservation in hedgehog signaling: induction of a chicken patched homolog by Sonic hedgehog in the developing limb. Development122:1225-1233.
20. Niswander L, Jeffrey S, Martin GR, Tickle C. 1994. A positive feedback loop coordinates growth and patterning in the vertebrate limb. Nature371:609-612.
21. Nybakken K, Vokes SA, Lin TY, McMahon AP, Perrimon N. 2005. A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. Nat Genet37:1323-1332.
22. Panman L, Galli A, Lagarde N, Michos O, Soete G, Zuniga A, Zeller R. 2006. Differential regulation of gene expression in the digit forming area of the mouse limb bud by SHH and gremlin 1/FGF-mediated epithelial-mesenchymal signalling. Development133:3419-3428.
23. Pastrana DV, Tolstov YL, Becker JC, Moore PS, Chang Y, Buck CB. 2009. Quantitation of human seroresponsiveness to Merkel cell polyomavirus. PLoS Pathog5:e1000578.
24. Paulsen DF, Solursh M. 1988. Microtiter micromass cultures of limb-bud mesenchymal cells. In Vitro Cell Dev Biol24:138-147.
25. Pizette S, Abate-Shen C, Niswander L. 2001. BMP controls proximodistal outgrowth, via induction of the apical ectodermal ridge, and dorsoventral patterning in the vertebrate limb. Development128:4463-4474.
26. Rabinowitz AH, Vokes SA. 2012. Integration of the transcriptional networks regulating limb morphogenesis. Dev Biol368:165-180.
27. Rosello-Diez A, Ros MA, Torres M. 2011. Diffusible signals, not autonomous mechanisms, determine the main proximodistal limb subdivision. Science332:1086-1088.
28. Sinha S, Chen JK. 2006. Purmorphamine activates the Hedgehog pathway by targeting Smoothened. Nat Chem Biol2:29-30.
29. Tannous BA, Kim DE, Fernandez JL, Weissleder R, Breakefield XO. 2005. Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Mol Ther11:435-443.
30. ten Berge D, Brugmann SA, Helms JA, Nusse R. 2008. Wnt and FGF signals interact to coordinate growth with cell fate specification during limb development. Development135:3247-3257.
31. te Welscher P, Zuniga A, Kuijper S, Drenth T, Goedemans HJ, Meijlink F, Zeller R. 2002. Progression of vertebrate limb development through SHH-mediated counteraction of GLI3. Science298:827-830.
32. Trevino C, Anderson R, Landry M, Konig G, Tonthat B, Shi C, Muneoka K. 1993. MPLB-2: a posterior signaling cell line derived from the mouse limb bud. Prog Clin Biol Res383A:295-304.
33. Vokes SA, Ji H, McCuine S, Tenzen T, Giles S, Zhong S, Longabaugh WJ, Davidson EH, Wong WH, McMahon AP. 2007. Genomic characterization of Gli-activator targets in sonic hedgehog-mediated neural patterning. Development134:1977-1989.
34. Vokes SA, Ji H, Wong WH, McMahon AP. 2008. A genome-scale analysis of the cis-regulatory circuitry underlying sonic hedgehog-mediated patterning of the mammalian limb. Genes Dev22:2651-2663.
35. Yuen CM, Rodda SJ, Vokes SA, McMahon AP, Liu DR. 2006. Control of transcription factor activity and osteoblast differentiation in mammalian cells using an evolved small-molecule-dependent intein. J Am Chem Soc128:8939-8946.
36. Zhu J, Nakamura E, Nguyen MT, Bao X, Akiyama H, Mackem S. 2008. Uncoupling Sonic hedgehog control of pattern and expansion of the developing limb bud. Dev Cell14:624-632.