Luciferase-based reporter to monitor the transcriptional activity of the SIRT3 promoter

Methods in Enzymology

Volumn 543, Issue , 2014, Pages 141-163

  Kyle Satterstrom, F ^a,b   Haigis, Marcia C ^b  

^a HARVARD UNIVERSITY   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Caloric restriction mimetic; Luciferase based reporter plasmid; Oncometabolism; Promoter analysis; Rapamycin; SIRT3

Indexed keywords

ESTROGEN RELATED RECEPTOR ALPHA; LUCIFERASE; MAMMALIAN TARGET OF RAPAMYCIN; RAPAMYCIN; SIRTUIN 3; TRANSCRIPTION FACTOR; PRIMER DNA; SIRT3 PROTEIN, HUMAN;

ARTICLE; CANCER CELL; CARCINOGENESIS; GENE ACTIVATION; GENE EXPRESSION; GENETIC TRANSCRIPTION; GLYCOLYSIS; HEK293 CELL LINE; HUMAN; HUMAN CELL; MOLECULAR CLONING; OXIDATIVE STRESS; PRIORITY JOURNAL; PROMOTER REGION; SIRT3 GENE; SITE DIRECTED MUTAGENESIS; TRANSACTIVATION; GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; REPORTER GENE; WESTERN BLOTTING;

BASE SEQUENCE; BLOTTING, WESTERN; DNA PRIMERS; GENES, REPORTER; HEK293 CELLS; HUMANS; LUCIFERASES; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; SIRTUIN 3; TRANSCRIPTION, GENETIC;

EID: 84902209678     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-801329-8.00007-6     Document Type: Chapter

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