Single-step antibody-based affinity cryo-electron microscopy for imaging and structural analysis of macromolecular assemblies

Journal of Structural Biology

Volumn 187, Issue 1, 2014, Pages 1-9

  Yu, Guimei ^a   Vago, Frank ^a   Zhang, Dongsheng ^b,c   Snyder, Jonathan E ^a   Yan, Rui ^a   Zhang, Ci ^a   Benjamin, Christopher ^d   Jiang, Xi ^b,c   Kuhn, Richard J ^a   Serwer, Philip ^e   Thompson, David H ^d   Jiang, Wen ^a  

^a PURDUE UNIVERSITY   (United States)

^b CINCINNATI CHILDREN'S HOSPITAL MEDICAL CENTER   (United States)

^c UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

^d PURDUE UNIVERSITY   (United States)

^e UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

Author keywords

3D reconstruction; Affinity cryo EM; Affinity grid; Antibodies; Bacteriophage T7; Single particle cryo EM

Indexed keywords

ANTIBODY; CELL EXTRACT; GENOMIC DNA; PROTEIN A; STAPHYLOCOCCUS PROTEIN A;

ARTICLE; BACTERIOPHAGE T7; CELL CULTURE; CELL LYSATE; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; ESCHERICHIA COLI; IMAGE PROCESSING; IMMUNOELECTRON MICROSCOPY; MACROMOLECULE; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; RIBOSOME; SINDBIS VIRUS; ANTIBODY AFFINITY; CALICIVIRUS; CHEMISTRY; DEVICES; ENTEROBACTERIA PHAGE T7; LARGE RIBOSOMAL SUBUNIT; PROCEDURES; ULTRASTRUCTURE;

ESCHERICHIA COLI; SINDBIS VIRUS;

ANTIBODIES; ANTIBODY AFFINITY; BACTERIOPHAGE T7; CALICIVIRIDAE; CRYOELECTRON MICROSCOPY; ESCHERICHIA COLI; RIBOSOME SUBUNITS, LARGE, BACTERIAL; SINDBIS VIRUS; STAPHYLOCOCCAL PROTEIN A;

EID: 84902155565     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2014.04.006     Document Type: Article

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