Monolayer purification: A rapid method for isolating protein complexes for single-particle electron microscopy

Proceedings of the National Academy of Sciences of the United States of America

Volumn 105, Issue 12, 2008, Pages 4703-4708

  Kelly, Deborah F ^a   Dukovski, Danijela ^a   Walz, Thomas ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Affinity purification; Cryoelectron microscopy; Lipid monolayer

Indexed keywords

CELL EXTRACT; IMIDAZOLE; NICKEL; NITRILOTRIACETIC ACID; TRANSFERRIN; TRANSFERRIN RECEPTOR; HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; MULTIPROTEIN COMPLEX; OLIGOPEPTIDE; RIBOSOME PROTEIN; RIBOSOME PROTEIN L3; UNCLASSIFIED DRUG;

ARTICLE; CELL LYSATE; ELECTRON MICROSCOPY; IMAGE RECONSTRUCTION; INSECT; LIPID MONOLAYER; MAMMAL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ISOLATION; PURIFICATION; RIBOSOME SUBUNIT; SENSITIVITY AND SPECIFICITY; ADSORPTION; ANIMAL; CHEMISTRY; CRYOELECTRON MICROSCOPY; ISOLATION AND PURIFICATION; LIPID METABOLISM; METABOLISM; METHODOLOGY; RIBOSOME; ULTRASTRUCTURE;

ESCHERICHIA COLI; HEXAPODA; MAMMALIA;

ADSORPTION; ANIMALS; CELL EXTRACTS; CHEMISTRY, ANALYTICAL; CRYOELECTRON MICROSCOPY; HISTIDINE; LIPID METABOLISM; MULTIPROTEIN COMPLEXES; NICKEL; NITRILOTRIACETIC ACID; OLIGOPEPTIDES; RECEPTORS, TRANSFERRIN; RIBOSOMAL PROTEINS; RIBOSOMES; TRANSFERRIN;

EID: 42449146663     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0800867105     Document Type: Article

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