Metabolic engineering for L-glutamine overproduction by using dna gyrase mutations in Escherichia coli

Applied and Environmental Microbiology

Volumn 79, Issue 9, 2013, Pages 3033-3039

  Hayashi, Mikiro ^a   Tabata, Kazuhiko ^a  

^a Kyowa Hakko Bio Co   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMOSOMAL DNAS; DEGRADATION ACTIVITY; DOUBLE STRANDED DNA; FLANKING REGIONS; MUTATION ANALYSIS; STRAIN DEVELOPMENT; TOPOISOMERASE I; TOPOISOMERASE IV;

DNA; ENCODING (SYMBOLS); ESCHERICHIA COLI; METABOLIC ENGINEERING;

AMINO ACIDS;

AMINO ACID; BACTERIOLOGY; FECAL COLIFORM; GENE EXPRESSION; GENETIC ENGINEERING; MUTATION;

ESCHERICHIA COLI; ESCHERICHIA COLI K12;

BACTERIAL DNA; BACTERIAL PROTEIN; DNA TOPOISOMERASE (ATP HYDROLYSING); ESCHERICHIA COLI PROTEIN; GLUTAMINE; THIOL ESTER HYDROLASE; YEIG PROTEIN, E COLI;

BACTERIAL GENOME; BIOSYNTHESIS; ENZYMOLOGY; ESCHERICHIA COLI K 12; GENETICS; METABOLIC ENGINEERING; METABOLISM; MICROBIAL SENSITIVITY TEST; MUTATION; PHENOTYPE;

BACTERIAL PROTEINS; BIOSYNTHETIC PATHWAYS; DNA GYRASE; DNA, BACTERIAL; ESCHERICHIA COLI K12; ESCHERICHIA COLI PROTEINS; GENOME, BACTERIAL; GLUTAMINE; METABOLIC ENGINEERING; MICROBIAL SENSITIVITY TESTS; MUTATION; PHENOTYPE; THIOLESTER HYDROLASES;

EID: 84876309101     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.03994-12     Document Type: Article

References (27)

1. Ahn WS, Antoniewicz MR. 2012. Towards dynamic metabolic flux analysis in CHO cell cultures. Biotechnol. J. 7:61-74.
2. Coëffier M, Déchelotte P. 2005. The role of glutamine in intensive care unit patients: mechanisms of action and clinical outcome. Nutr. Rev. 63: 65-69.
3. Tabata K, Hashimoto S. 2007. Fermentative production of L-alanyl-Lglutamine by a metabolically engineered Escherichia coli strain expressing L-amino acid α-ligase. Appl. Environ. Microbiol. 73:6378-6385.
4. Ikeda M. 2003. Amino acid production processes, p 1-35. In Faurie R, Thommel J (ed), Advances in biochemical engineering/biotechnology, vol 79. Microbial production of L-amino acids. Springer-Verlag, Berlin, Germany.
5. Ikeda M, Ohnishi J, Hayashi M, Mitsuhashi S. 2006. A genome-based approach to create a minimally mutated Corynebacterium glutamicum strain for efficient L-lysine production. J. Ind. Microbiol. Biotechnol. 33: 610-615.
6. Ikeda M, Mitsuhashi S, Tanaka K, Hayashi M. 2009. Reengineering of a Corynebacterium glutamicum L-arginine and L-citrulline producer. Appl. Environ. Microbiol. 75:1635-1641.
7. Okamoto K, Kino K, Ikeda M. 1997. Hyperproduction of L-threonine by an Escherichia coli mutant with impaired L-threonine uptake. Biosci. Biotechnol. Biochem. 61:1877-1882.
8. Datsenko KA, Wanner BL. 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U. S. A. 97:6640-6645.
9. Casadaban MJ, Chou J, Cohen SN. 1980. In vitro gene fusions that join an enzymatically active β-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J. Bacteriol. 143: 971-980.
10. Mizoguchi H, Tanaka-Masuda K, Mori H. 2007. A simple method for multiple modification of the Escherichia coli K-12 chromosome. Biosci. Biotechnol. Biochem. 71:2905-2911.
11. Sambrook J, Russell DW. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
12. Griffith KL, Wolf RE, Jr. 2002. Measuring β-galactosidase activity in bacteria: cell growth, permeabilization, and enzyme assays in 96-well arrays. Biochem. Biophys. Res. Commun. 290:397-402.
13. Auriol C, Bestel-Corre G, Claude JB, Soucaille P, Meynial-Salles I. 2011. Stress-induced evolution of Escherichia coli points to original concepts in respiratory cofactor selectivity. Proc. Natl. Acad. Sci. U. S. A. 108:1278-1283.
14. Herring CD, Palsson BØ. 2007. An evaluation of comparative genome sequencing (CGS) by comparing two previously-sequenced bacterial genomes. BMC Genomics 8:274. doi:10.1186/1471-2164-8-274.
15. Gonzalez CF, Proudfoot M, Brown G, Korniyenko Y, Mori H, Savchenko AV, Yakunin AF. 2006. Molecular basis of formaldehyde detoxification. Characterization of two S-formylglutathione hydrolases from Escherichia coli, FrmB and YeiG. J. Biol. Chem. 281:14514-14522.
16. Tretter EM, Berger JM. 2012. Mechanisms for defining supercoiling set point of DNA gyrase orthologs: II. The shape of the GyrA subunit C-terminal domain (CTD) is not a sole determinant for controlling supercoiling efficiency. J. Biol. Chem. 287:18645-18654.
17. Free A, Dorman CJ. 1994. Escherichia coli tyrT gene transcription is sensitive to DNA supercoiling in its native chromosomal context: effect of DNA topoisomerase IV overexpression on tyrT promoter function. Mol. Microbiol. 14:151-161.
18. Travers A, Muskhelishvili G. 2005. DNA supercoiling-a global transcriptional regulator for enterobacterial growth? Nat. Rev. Microbiol. 3:157-169.
19. Ashiuchi M, Kuwana E, Yamamoto T, Komatsu K, Soda K, Misono H. 2002. Glutamate racemase is an endogenousDNAgyrase inhibitor. J. Biol. Chem. 277:39070-39073.
20. Jensen KF. 1993. The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. J. Bacteriol. 175:3401-3407.
21. Andersen DC, Swartz J, Ryll T, Lin N, Snedecor B. 2001. Metabolic oscillations in an E. coli fermentation. Biotechnol. Bioeng. 75:212-218.
22. Smulski DR, Huang LL, McCluskey MP, Reeve MJ, Vollmer AC, Van Dyk TK, LaRossa RA. 2001. Combined, functional genomic-biochemical approach to intermediary metabolism: interaction of acivicin, a glutamine amidotransferase inhibitor, with Escherichia coli K-12. J. Bacteriol. 183: 3353-3364.
23. Brown G, Singer A, Proudfoot M, Skarina T, Kim Y, Chang C, Dementieva I, Kuznetsova E, Gonzalez CF, Joachimiak A, Savchenko A, Yakunin AF. 2008. Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis. Biochemistry 47: 5724-5735.
24. Goss TJ, Perez-Matos A, Bender RA. 2001. Roles of glutamate synthase, gltBD, and gltF in nitrogen metabolism of Escherichia coli and Klebsiella aerogenes. J. Bacteriol. 183:6607-6619.
25. Ohnishi J, Mitsuhashi S, Hayashi M, Ando S, Yokoi H, Ochiai K, Ikeda M. 2002. A novel methodology employing Corynebacterium glutamicum genome information to generate a new L-lysine-producing mutant. Appl. Microbiol. Biotechnol. 58:217-223.
26. Rudd KE, Menzel R. 1987. his operons of Escherichia coli and Salmonella typhimurium are regulated by DNA supercoiling. Proc. Natl. Acad. Sci. U. S. A. 84:517-521.
27. Franke I, Resch A, Dassler T, Maier T, Böck A. 2003. YfiK from Escherichia coli promotes export of O-acetylserine and cysteine. J. Bacteriol. 185:1161-1166.